Xiaoqing He, Yi Wu, Peng Shu, Yanpeng Yin, Heng Deng, Shiyu Ying, Mengbo Wu, Panpan Zhao, Tengbo Huang, Julien Pirrello, Yang Zhang, Don Grierson, Zhenhui Zhong, Yiguo Hong, Mondher Bouzayen, Mingchun Liu
{"title":"SlPLT6通过调节组蛋白乙酰化和甲基化调控番茄的成熟起始和品质性状。","authors":"Xiaoqing He, Yi Wu, Peng Shu, Yanpeng Yin, Heng Deng, Shiyu Ying, Mengbo Wu, Panpan Zhao, Tengbo Huang, Julien Pirrello, Yang Zhang, Don Grierson, Zhenhui Zhong, Yiguo Hong, Mondher Bouzayen, Mingchun Liu","doi":"10.1073/pnas.2503732122","DOIUrl":null,"url":null,"abstract":"<p><p>Improving fruit growth and quality without compromising yield is a highly sought-after goal in crop breeding. Here, we report that an EAR motif-containing transcription factor SlPLT6 controls the onset of fruit ripening and quality traits in tomato. <i>SlPLT6</i> knockout caused precocious ripening initiation and enhanced fruit qualities without penalizing yield in two tomato cultivars. SlPLT6 represses genes involved in ripening initiation and major quality traits by directly binding to their promoters. Moreover, SlPLT6 connects two repressive mechanisms by recruiting Histone Deacetylase 1 via its C-terminal EAR motif and LIKE HETEROCHROMATIN PROTEIN 1b through the first AP2 domain. Furthermore, Sly-miR159 acts upstream of SlPLT6 to release its repression. Taken together, our data provide insights into the operating mode of SlPLT6 in modulating fruit ripening and quality traits and define a unique gene for targeted crop improvement aimed at upgrading fruit quality without yield penalty.</p>","PeriodicalId":20548,"journal":{"name":"Proceedings of the National Academy of Sciences of the United States of America","volume":"122 24","pages":"e2503732122"},"PeriodicalIF":9.1000,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12184660/pdf/","citationCount":"0","resultStr":"{\"title\":\"SlPLT6 controls ripening initiation and quality traits through modulation of histone acetylation and methylation in tomato.\",\"authors\":\"Xiaoqing He, Yi Wu, Peng Shu, Yanpeng Yin, Heng Deng, Shiyu Ying, Mengbo Wu, Panpan Zhao, Tengbo Huang, Julien Pirrello, Yang Zhang, Don Grierson, Zhenhui Zhong, Yiguo Hong, Mondher Bouzayen, Mingchun Liu\",\"doi\":\"10.1073/pnas.2503732122\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Improving fruit growth and quality without compromising yield is a highly sought-after goal in crop breeding. Here, we report that an EAR motif-containing transcription factor SlPLT6 controls the onset of fruit ripening and quality traits in tomato. <i>SlPLT6</i> knockout caused precocious ripening initiation and enhanced fruit qualities without penalizing yield in two tomato cultivars. SlPLT6 represses genes involved in ripening initiation and major quality traits by directly binding to their promoters. Moreover, SlPLT6 connects two repressive mechanisms by recruiting Histone Deacetylase 1 via its C-terminal EAR motif and LIKE HETEROCHROMATIN PROTEIN 1b through the first AP2 domain. Furthermore, Sly-miR159 acts upstream of SlPLT6 to release its repression. Taken together, our data provide insights into the operating mode of SlPLT6 in modulating fruit ripening and quality traits and define a unique gene for targeted crop improvement aimed at upgrading fruit quality without yield penalty.</p>\",\"PeriodicalId\":20548,\"journal\":{\"name\":\"Proceedings of the National Academy of Sciences of the United States of America\",\"volume\":\"122 24\",\"pages\":\"e2503732122\"},\"PeriodicalIF\":9.1000,\"publicationDate\":\"2025-06-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12184660/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the National Academy of Sciences of the United States of America\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://doi.org/10.1073/pnas.2503732122\",\"RegionNum\":1,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/10 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the National Academy of Sciences of the United States of America","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1073/pnas.2503732122","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/10 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
SlPLT6 controls ripening initiation and quality traits through modulation of histone acetylation and methylation in tomato.
Improving fruit growth and quality without compromising yield is a highly sought-after goal in crop breeding. Here, we report that an EAR motif-containing transcription factor SlPLT6 controls the onset of fruit ripening and quality traits in tomato. SlPLT6 knockout caused precocious ripening initiation and enhanced fruit qualities without penalizing yield in two tomato cultivars. SlPLT6 represses genes involved in ripening initiation and major quality traits by directly binding to their promoters. Moreover, SlPLT6 connects two repressive mechanisms by recruiting Histone Deacetylase 1 via its C-terminal EAR motif and LIKE HETEROCHROMATIN PROTEIN 1b through the first AP2 domain. Furthermore, Sly-miR159 acts upstream of SlPLT6 to release its repression. Taken together, our data provide insights into the operating mode of SlPLT6 in modulating fruit ripening and quality traits and define a unique gene for targeted crop improvement aimed at upgrading fruit quality without yield penalty.
期刊介绍:
The Proceedings of the National Academy of Sciences (PNAS), a peer-reviewed journal of the National Academy of Sciences (NAS), serves as an authoritative source for high-impact, original research across the biological, physical, and social sciences. With a global scope, the journal welcomes submissions from researchers worldwide, making it an inclusive platform for advancing scientific knowledge.