{"title":"双组学揭示了玻璃化小鼠卵母细胞的时间翻译恢复景观和低温损伤修复机制。","authors":"Shaohong Zhuang, Xiaoting Diao, Rui Xiang, Jiana Huang, Jiahui Pang, Na Lu, Qiqi Liang, Xinyi Pan, Rang Liu, Cong Fang, Xiaoyan Liang, Wenlin Peng, Haitao Zeng","doi":"10.1007/s10815-025-03482-w","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>The efficacy of oocyte cryopreservation still requires enhancement. Current understanding of cryodamage is largely limited to the organelle level, and the impact of freezing on oocyte gene expression remains unclear.</p><p><strong>Methods: </strong>In this study, we employed an innovative dual-omics approach to assess the transcriptional and translational profiles of mouse metaphase II (MII) oocytes during the initial 4 h post-thaw.</p><p><strong>Results: </strong>Our mapping of the translational recovery in vitrified mouse oocytes post-thaw revealed a critical 2-h window that is optimal for recovery. We confirmed the mitochondrial damage associated with vitrification and identified the activation of autophagy and proteasomal degradation during this period. Additionally, our analysis indicates that vitrified oocytes have another repair response to counteract cryoinjury, involving spindle remodeling and membrane recycling.</p><p><strong>Conclusions: </strong>These findings can guide future efforts to improve oocyte vitrification outcomes by improving repair processes and not focusing solely on the damage processes.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dual-omics reveals temporal translational recovery landscapes and cryodamage repair mechanisms in vitrified mouse oocytes.\",\"authors\":\"Shaohong Zhuang, Xiaoting Diao, Rui Xiang, Jiana Huang, Jiahui Pang, Na Lu, Qiqi Liang, Xinyi Pan, Rang Liu, Cong Fang, Xiaoyan Liang, Wenlin Peng, Haitao Zeng\",\"doi\":\"10.1007/s10815-025-03482-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>The efficacy of oocyte cryopreservation still requires enhancement. Current understanding of cryodamage is largely limited to the organelle level, and the impact of freezing on oocyte gene expression remains unclear.</p><p><strong>Methods: </strong>In this study, we employed an innovative dual-omics approach to assess the transcriptional and translational profiles of mouse metaphase II (MII) oocytes during the initial 4 h post-thaw.</p><p><strong>Results: </strong>Our mapping of the translational recovery in vitrified mouse oocytes post-thaw revealed a critical 2-h window that is optimal for recovery. We confirmed the mitochondrial damage associated with vitrification and identified the activation of autophagy and proteasomal degradation during this period. Additionally, our analysis indicates that vitrified oocytes have another repair response to counteract cryoinjury, involving spindle remodeling and membrane recycling.</p><p><strong>Conclusions: </strong>These findings can guide future efforts to improve oocyte vitrification outcomes by improving repair processes and not focusing solely on the damage processes.</p>\",\"PeriodicalId\":15246,\"journal\":{\"name\":\"Journal of Assisted Reproduction and Genetics\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-06-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Assisted Reproduction and Genetics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s10815-025-03482-w\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Assisted Reproduction and Genetics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10815-025-03482-w","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Dual-omics reveals temporal translational recovery landscapes and cryodamage repair mechanisms in vitrified mouse oocytes.
Purpose: The efficacy of oocyte cryopreservation still requires enhancement. Current understanding of cryodamage is largely limited to the organelle level, and the impact of freezing on oocyte gene expression remains unclear.
Methods: In this study, we employed an innovative dual-omics approach to assess the transcriptional and translational profiles of mouse metaphase II (MII) oocytes during the initial 4 h post-thaw.
Results: Our mapping of the translational recovery in vitrified mouse oocytes post-thaw revealed a critical 2-h window that is optimal for recovery. We confirmed the mitochondrial damage associated with vitrification and identified the activation of autophagy and proteasomal degradation during this period. Additionally, our analysis indicates that vitrified oocytes have another repair response to counteract cryoinjury, involving spindle remodeling and membrane recycling.
Conclusions: These findings can guide future efforts to improve oocyte vitrification outcomes by improving repair processes and not focusing solely on the damage processes.
期刊介绍：
The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species.
The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
