{"title":"一种用于人类精子冷冻保存的小液滴冷冻新方法：最大限度地利用有限的样本。","authors":"Yael Harir, Rona Halevy Amiran, Miriam Almagor, Sheila Fieldust, Zeev Shoham, Yuval Or","doi":"10.1007/s10815-025-03532-3","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>The study aims to evaluate and compare sperm progressive motility before and after cryopreservation using the conventional slow freezing technique versus a novel small droplets freezing method. This research seeks to determine the optimal freezing technique, particularly for preserving unique and irreplaceable sperm samples.</p><p><strong>Methods: </strong>Seventy-five sperm samples were divided and cryopreserved using two techniques: (1) the classic slow freezing method and (2) the small droplets freezing method. After thawing, sperm progressive motility was assessed and compared between the two methods.</p><p><strong>Results: </strong>No significant difference in sperm progressive motility was observed between the two techniques. The mean post-thaw progressive motility was 22.9% for the classical method and 23.64% for the small droplets method (p = 0.74), indicating comparable outcomes.</p><p><strong>Conclusion: </strong>Cryopreservation in small droplets maintains sperm progressive motility similarly to conventional slow freezing. This technique allows for more efficient utilization of limited sperm samples, making it particularly valuable for posthumous sperm retrieval, fertility preservation in oncological patients, and cases where sperm availability is extremely limited.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A novel small droplets freezing method for human sperm cryopreservation: maximizing utilization of limited samples.\",\"authors\":\"Yael Harir, Rona Halevy Amiran, Miriam Almagor, Sheila Fieldust, Zeev Shoham, Yuval Or\",\"doi\":\"10.1007/s10815-025-03532-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>The study aims to evaluate and compare sperm progressive motility before and after cryopreservation using the conventional slow freezing technique versus a novel small droplets freezing method. This research seeks to determine the optimal freezing technique, particularly for preserving unique and irreplaceable sperm samples.</p><p><strong>Methods: </strong>Seventy-five sperm samples were divided and cryopreserved using two techniques: (1) the classic slow freezing method and (2) the small droplets freezing method. After thawing, sperm progressive motility was assessed and compared between the two methods.</p><p><strong>Results: </strong>No significant difference in sperm progressive motility was observed between the two techniques. The mean post-thaw progressive motility was 22.9% for the classical method and 23.64% for the small droplets method (p = 0.74), indicating comparable outcomes.</p><p><strong>Conclusion: </strong>Cryopreservation in small droplets maintains sperm progressive motility similarly to conventional slow freezing. This technique allows for more efficient utilization of limited sperm samples, making it particularly valuable for posthumous sperm retrieval, fertility preservation in oncological patients, and cases where sperm availability is extremely limited.</p>\",\"PeriodicalId\":15246,\"journal\":{\"name\":\"Journal of Assisted Reproduction and Genetics\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-06-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Assisted Reproduction and Genetics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s10815-025-03532-3\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Assisted Reproduction and Genetics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10815-025-03532-3","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
A novel small droplets freezing method for human sperm cryopreservation: maximizing utilization of limited samples.
Purpose: The study aims to evaluate and compare sperm progressive motility before and after cryopreservation using the conventional slow freezing technique versus a novel small droplets freezing method. This research seeks to determine the optimal freezing technique, particularly for preserving unique and irreplaceable sperm samples.
Methods: Seventy-five sperm samples were divided and cryopreserved using two techniques: (1) the classic slow freezing method and (2) the small droplets freezing method. After thawing, sperm progressive motility was assessed and compared between the two methods.
Results: No significant difference in sperm progressive motility was observed between the two techniques. The mean post-thaw progressive motility was 22.9% for the classical method and 23.64% for the small droplets method (p = 0.74), indicating comparable outcomes.
Conclusion: Cryopreservation in small droplets maintains sperm progressive motility similarly to conventional slow freezing. This technique allows for more efficient utilization of limited sperm samples, making it particularly valuable for posthumous sperm retrieval, fertility preservation in oncological patients, and cases where sperm availability is extremely limited.
期刊介绍：
The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species.
The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.
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