{"title":"有丝分裂基因组折叠。","authors":"Tatsuya Hirano","doi":"10.1083/jcb.202504075","DOIUrl":null,"url":null,"abstract":"<p><p>Mitotic genome folding, or mitotic chromosome assembly, is essential for the faithful segregation of genetic information into daughter cells. While this process was once thought to be highly complex, requiring a myriad of protein components, recent studies have begun to revise this conventional view. An emerging view is that the core reaction of mitotic genome folding is mediated by a dynamic interplay of a limited number of structural components, namely, condensins, topoisomerase II (topo II), and histones. Condensins and topo II are two distinct classes of ATPases that cooperate to actively form and manipulate DNA loops, both accumulating at the central axial regions of the resulting chromosomes. In contrast, nucleosomes and linker histones help to compact DNA loops by cooperating and competing with the action of these ATPases. In this review, I will focus on the recent advances in the field, with an emphasis on the mechanistic aspects of mitotic genome folding.</p>","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"224 7","pages":""},"PeriodicalIF":7.4000,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150863/pdf/","citationCount":"0","resultStr":"{\"title\":\"Mitotic genome folding.\",\"authors\":\"Tatsuya Hirano\",\"doi\":\"10.1083/jcb.202504075\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mitotic genome folding, or mitotic chromosome assembly, is essential for the faithful segregation of genetic information into daughter cells. While this process was once thought to be highly complex, requiring a myriad of protein components, recent studies have begun to revise this conventional view. An emerging view is that the core reaction of mitotic genome folding is mediated by a dynamic interplay of a limited number of structural components, namely, condensins, topoisomerase II (topo II), and histones. Condensins and topo II are two distinct classes of ATPases that cooperate to actively form and manipulate DNA loops, both accumulating at the central axial regions of the resulting chromosomes. In contrast, nucleosomes and linker histones help to compact DNA loops by cooperating and competing with the action of these ATPases. In this review, I will focus on the recent advances in the field, with an emphasis on the mechanistic aspects of mitotic genome folding.</p>\",\"PeriodicalId\":15211,\"journal\":{\"name\":\"Journal of Cell Biology\",\"volume\":\"224 7\",\"pages\":\"\"},\"PeriodicalIF\":7.4000,\"publicationDate\":\"2025-07-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150863/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Cell Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1083/jcb.202504075\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/10 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cell Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1083/jcb.202504075","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/10 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Mitotic genome folding, or mitotic chromosome assembly, is essential for the faithful segregation of genetic information into daughter cells. While this process was once thought to be highly complex, requiring a myriad of protein components, recent studies have begun to revise this conventional view. An emerging view is that the core reaction of mitotic genome folding is mediated by a dynamic interplay of a limited number of structural components, namely, condensins, topoisomerase II (topo II), and histones. Condensins and topo II are two distinct classes of ATPases that cooperate to actively form and manipulate DNA loops, both accumulating at the central axial regions of the resulting chromosomes. In contrast, nucleosomes and linker histones help to compact DNA loops by cooperating and competing with the action of these ATPases. In this review, I will focus on the recent advances in the field, with an emphasis on the mechanistic aspects of mitotic genome folding.
期刊介绍:
The Journal of Cell Biology (JCB) is a comprehensive journal dedicated to publishing original discoveries across all realms of cell biology. We invite papers presenting novel cellular or molecular advancements in various domains of basic cell biology, along with applied cell biology research in diverse systems such as immunology, neurobiology, metabolism, virology, developmental biology, and plant biology. We enthusiastically welcome submissions showcasing significant findings of interest to cell biologists, irrespective of the experimental approach.