枸杞多糖的化学结构与老龄秀丽隐杆线虫肠道菌群组成有关

IF 11 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids Pub Date : 2025-06-04 DOI:10.1016/j.foodhyd.2025.111608

Yan Wei , Yinxiu Jiang , Mengqiu Liu , Xinrun Zhang , Sheng Guo , Shulan Su , Yang Pan , Zheng Qiu , Xia Zhang , Jin-ao Duan , Fang Zhang

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引用次数: 0

摘要

肠道菌群组成的恶化与衰老的开始和进展密切相关。天然生物活性多糖已成为调节微生物群组成和延缓衰老的中心焦点。本研究采用Fehling沉淀法纯化了Lycii fructus （LFPs）中的三种杂多糖，分别为FP、FSP和FSS，并通过广泛的结构解析策略进行了物理化学表征，包括分子量分布分析、单糖组成测定、糖苷连锁位置分析和核磁共振光谱分析。lfp含有不同比例的线性高半乳糖酸（HG）片段，鼠李糖半乳糖酸- i （RG-I）短序列，沿RG-I轴具有中性阿拉伯糖、半乳聚糖和阿拉伯半乳聚糖侧链。外源性给药lfp显著延长秀丽隐杆线虫的寿命，提示其潜在的抗衰老作用。在衰老的秀丽隐杆线虫中，lfp通过恢复微生物群落组成和改变微生物相互作用来减轻肠道微生物群失调。此外，lfp对肠道微生物群的调节是通过结构选择机制而不是随机过程进行的。这些发现增强了我们对lfp的化学结构与其对肠道微生物群的生物学效应之间内在联系的理解。这项工作与我们之前的报告一致，强调了lfp作为对抗衰老和肠道微生物群相关疾病的自然干预措施的潜在未来应用。

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Chemical structures of Lycii fructus polysaccharides tailored the gut microbiota composition of aged Caenorhabditis elegans

Deterioration in gut microbiota composition is closely associated with the initiation and progression of aging. Natural bioactive polysaccharides have emerged as a central focus for modulating microbiota composition and delaying senescence. In this study, three heteropolysaccharide fractions from Lycii fructus (LFPs), designated FP, FSP, and FSS, were purified using Fehling precipitation and characterized physiochemically through an extensive structural elucidation strategy, including molecular weight distribution analysis, monosaccharide composition determination, glycosidic linkage position analysis, and NMR spectroscopy. LFPs contained varying ratios of linear homogalacturonan (HG) fragments, short rhamnogalacturonan-I (RG-I) sequences with neutral arabinan, galactan, and arabinogalactan side chains along the RG-I axis. Exogenous administration of LFPs significantly extended the lifespan of C. elegans, suggesting their potential anti-aging effects. In aged C. elegans, LFPs mitigated gut microbiota dysbiosis by restoring microbial community composition and altering microbial interactions. Moreover, the modulation of gut microbiota by LFPs was tailored by structure-selective mechanisms rather than stochastic processes. These findings enhance our understanding of the intrinsic connection between the chemical structures of LFPs and their biological effects on gut microbiota. This work aligns with our previous reports underscoring the potential future applications of LFPs as natural interventions to counteract aging and gut microbiota-related disorders.

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来源期刊

Food Hydrocolloids 工程技术-食品科技

CiteScore

19.90

自引率

14.00%

发文量

871

审稿时长

37 days

期刊介绍： Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.