通过dcas13引导的工程乙酰转移酶靶向RNA乙酰化

IF 12.9 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nature chemical biology Pub Date : 2025-06-10 DOI:10.1038/s41589-025-01923-2

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引用次数: 0

摘要

我们开发了一个可编程的RNA乙酰化系统，通过将dCas13与工程NAT10变体融合，使n4 -乙酰胞苷（ac4C）能够在培养细胞和活体动物的靶RNA上进行稳健和特异性的安装。该系统促进了RNA乙酰化的功能研究，并揭示了ac4C在调节转录物亚细胞定位中的独特作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Targeted RNA acetylation via a dCas13-guided engineered acetyl-transferase

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Targeted RNA acetylation via a dCas13-guided engineered acetyl-transferase

We developed a programmable RNA acetylation system by fusing dCas13 with an engineered NAT10 variant, enabling robust and specific installation of N4-acetylcytidine (ac4C) on target RNAs in cultured cells and live animals. This system facilitated functional studies of RNA acetylation and revealed ac4C has a distinct role in regulating transcript subcellular localization.

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来源期刊

Nature chemical biology 生物-生化与分子生物学

CiteScore

23.90

自引率

1.40%

发文量

238

审稿时长

12 months

期刊介绍： Nature Chemical Biology stands as an esteemed international monthly journal, offering a prominent platform for the chemical biology community to showcase top-tier original research and commentary. Operating at the crossroads of chemistry, biology, and related disciplines, chemical biology utilizes scientific ideas and approaches to comprehend and manipulate biological systems with molecular precision. The journal embraces contributions from the growing community of chemical biologists, encompassing insights from chemists applying principles and tools to biological inquiries and biologists striving to comprehend and control molecular-level biological processes. We prioritize studies unveiling significant conceptual or practical advancements in areas where chemistry and biology intersect, emphasizing basic research, especially those reporting novel chemical or biological tools and offering profound molecular-level insights into underlying biological mechanisms. Nature Chemical Biology also welcomes manuscripts describing applied molecular studies at the chemistry-biology interface due to the broad utility of chemical biology approaches in manipulating or engineering biological systems. Irrespective of scientific focus, we actively seek submissions that creatively blend chemistry and biology, particularly those providing substantial conceptual or methodological breakthroughs with the potential to open innovative research avenues. The journal maintains a robust and impartial review process, emphasizing thorough chemical and biological characterization.