Georges Chabouh, Louise Denis, Myriam Abioui-Mourgues, Jean-Baptiste Deloges, Jacques Battaglia, Arthur Chavignon, Baptiste Heiles, Basile Pradier, Sylvain Bodard, Denis Vivien, Cyrille Orset, Olivier Couture
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3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline.
We present an open-source code for 3D super-resolution ultrasound imaging. Open-3DULM was applied to transcranial imaging in habituated awake mice. Comparative analysis reveals that isoflurane anesthesia induces significant vasodilation and increased cerebral blood flow (CBF) in veins, while only a subset of arteries exhibits these effects compared to the awake state. This method could serve as a reference for developing new types of 3D vascular quantifications, particularly emphasizing the importance of awake and 3D imaging for accurate cerebral blood flow measurement in neuroscience research.
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