同轴氦电喷雾用于x射线自由电子激光器的单粒子成像。

IF 2.5 3区物理与天体物理

Journal of Synchrotron Radiation Pub Date : 2025-07-01 Epub Date: 2025-06-06 DOI:10.1107/S1600577525003686

Safi Rafie-Zinedine, Joachim Schulz, Johan Bielecki, Michael Heymann

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引用次数: 0

摘要

x射线自由电子激光器的单粒子成像依赖于在室温下将纳米级大分子和粒子适当地注入气相。同轴液片策略大大扩展了合适样品的范围，包括从0到40000 μ S cm-1的电导率-与传统电喷雾相比，范围增加了约8倍。氦气室大气与工程气片相结合，保护气溶胶形成免受日冕放电的影响，并将背景噪声降低三倍以上。这些结果提出了新的途径，使将来要求更高的生物和材料科学样品能够用于单粒子成像。

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Coaxial helium electrospray for single-particle imaging at X-ray free electron lasers.

Single-particle imaging at X-ray free-electron lasers relies on suitable sample injection of nanoscale macromolecules and particles into the gas phase at room temperature. A coaxial liquid-sheet strategy considerably extended the range of suitable samples to include conductivities from zero to 40000 µS cm^-1 - a more than about an eightfold increase in range compared with conventional electrosprays. A helium chamber atmosphere in combination with an engineered gas-sheet protected aerosol formation against corona discharge and reduced background noise more than threefold. These results suggest new avenues to qualify ever more demanding biological and material science samples for single-particle imaging in the future.

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来源期刊

Journal of Synchrotron Radiation INSTRUMENTS & INSTRUMENTATIONOPTICS&-OPTICS

CiteScore

5.60

自引率

12.00%

发文量

289

审稿时长

1 months

期刊介绍： Synchrotron radiation research is rapidly expanding with many new sources of radiation being created globally. Synchrotron radiation plays a leading role in pure science and in emerging technologies. The Journal of Synchrotron Radiation provides comprehensive coverage of the entire field of synchrotron radiation and free-electron laser research including instrumentation, theory, computing and scientific applications in areas such as biology, nanoscience and materials science. Rapid publication ensures an up-to-date information resource for scientists and engineers in the field.