中国基因II型Akabane病毒株(TJ2016)在小鼠模型中的致病性分析。

IF 4 3区 医学 Q2 VIROLOGY
Jingjing Wang, Ruyang Yu, Fang Wei, Dongjie Chen, Shaoqiang Wu
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引用次数: 0

摘要

背景:Akabane病毒(AKAV)分为5个基因群(I ~ V),不同基因群毒株的致病性有显著差异。我们于2016年在中国分离到一株II基因组AKAV毒株TJ2016,但其毒力尚不清楚。在中国分离的其他基因II型菌株的致病潜力也尚未确定。本研究的目的是确定TJ2016的致病性。方法:昆明小鼠和Balb/c小鼠分别于7日龄和8周龄通过脑内(IC)、腹腔(IP)、皮下(SC)和肌内(IM)途径接种TJ2016。监测和分析接种小鼠的临床症状、病理改变和AKAV分布。结果:无论小鼠品系和年龄,通过IC途径接种病毒的小鼠死亡率为75% ~ 100%。通过IP途径接种病毒,乳鼠的死亡率为75% ~ 100%,而成年鼠没有死亡。通过SC和IM途径接种的小鼠全部存活至试验结束。AKAV只在实验结束前死亡或被安乐死的老鼠的大脑中被检测到。AKAV抗原仅在神经元细胞内可识别。仅在实验结束前死亡或安乐死的小鼠中观察到脑损伤,如小胶质细胞的增殖和浸润,淋巴细胞和巨噬细胞的血管周围弯曲(PVC),神经元变性/坏死,血管扩张和充血等。结论:我们通过不同途径接种乳鼠和成年鼠,表征了TJ2016的毒力,建立了实验小鼠模型,这对疫苗/药物的开发以及进一步研究病毒的发病机制具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pathogenicity analysis of a Chinese Genogroup II Akabane virus strain (TJ2016) in mouse models.

Background: Akabane virus (AKAV) is divided into five genogroups (I to V), and strains of different genogroups exhibit marked differences in pathogenicity. We isolated a genogroup II AKAV strain, TJ2016, in China in 2016, but its virulence remains unknown. The pathogenic potential of other genogroup II strains isolated in China also remains uncharacterized. The objectives of this study were to determine the pathogenicity of TJ2016.

Methods: Kunming or Balb/c mice at 7 days or 8 weeks of age were inoculated with TJ2016 by intracerebral (IC), intraperitoneal (IP), subcutaneous (SC), or intramuscular (IM) routes. Clinical signs, pathological alterations, and AKAV distributions in the inoculated mice were monitored and analyzed.

Results: Virus inoculations by the IC route resulted in 75% ~ 100% mortality of the inoculated mice regardless of the mouse strains or ages. Virus inoculations by the IP route killed 75% to 100% of the suckling mice but killed no adult mice. All the mice inoculated via SC and IM routes survived until the end of the trial. AKAV was detected only in the brains of the mice that died or were euthanized before the end of the experiment. The AKAV antigens were only identifiable within neuronal cells. Brain lesions such as proliferation and infiltration of microglial cells, perivascular cuffing (PVC) of lymphocytes and macrophages, neuronal degeneration/necrosis, vascular dilatation and congestion, etc., were observed only in the mice that died or were euthanized before the end of the experiment.

Conclusions: We characterized the virulence of TJ2016 by inoculating suckling and adult mice via different routes and established experimental mouse models, which holds significant implications for vaccine/drug development and further research on viral pathogenesis.

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来源期刊
Virology Journal
Virology Journal 医学-病毒学
CiteScore
7.40
自引率
2.10%
发文量
186
审稿时长
1 months
期刊介绍: Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.
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