Nik Mohd Noor Nik Zuraina, Nur Annisa Nasuha Mohd Sedi, Mohammed Dauda Goni, Suharni Mohamad, Mohd Nor Norazmi, Siti Suraiya
{"title":"马来西亚特异性结核分枝杆菌菌株SIT745/EAI1-MYS引起的肺结核中的干扰素- γ和白介素-10遗传多态性","authors":"Nik Mohd Noor Nik Zuraina, Nur Annisa Nasuha Mohd Sedi, Mohammed Dauda Goni, Suharni Mohamad, Mohd Nor Norazmi, Siti Suraiya","doi":"10.7717/peerj.19576","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Mycobacterium tuberculosis</i> is the causative agent of tuberculosis, which results in 1.5 million global deaths annually. Key factors influencing tuberculosis development encompass host genetic factors, genetic diversity within <i>M. tuberculosis</i>, and environmental conditions. The East-African-Indian (EAI) lineage is the predominant lineage in Southeast Asia, India, and East Africa. A recent finding has identified SIT745/EAI1-MYS, a sub-lineage of EAI, as the geographically specific <i>M. tuberculosis</i> strain in Malaysia.</p><p><strong>Methods: </strong>This study aimed to investigate the role of IFN-γ (+874) A/T and IL-10 (-1,082) A/G SNPs with tuberculosis (TB) disease susceptibility among patients infected with <i>M. tuberculosis</i> SIT745/EAI1-MYS strain. A cross-sectional study was conducted between three groups of subjects consisted of TB patients infected with <i>M. tuberculosis</i> SIT745/EAI1-MYS strain (<i>n</i> = 9), TB patients of non-<i>M. tuberculosis</i> SIT745/EAI1-MYS strains (<i>n</i> = 9), and healthy controls (<i>n</i> = 9). The genetic variation in IFN-γ (+874) A/T and IL-10 (-1,082) A/G SNPs were detected using allele-specific PCR and analyzed for their association with TB risk and severity.</p><p><strong>Results: </strong>The results indicated a higher frequency of the IFN-γ (+874) TT and IL-10 (-1,082) AG genotypes among TB patients compared to healthy controls. The IFN-γ (+874) AA and IL-10 (-1,082) AG genotypes were more prevalent among TB patients infected with the SIT745/EAI1-MYS <i>M. tuberculosis</i> strains compared to non-SIT745/EAI1-MYS, indicating a possible link between these genotypes and more severe TB symptoms. Although there is no significant correlation between the IFN-γ (+874) A/T and IL-10 (-1,082) A/G polymorphisms with the susceptibility or severity of TB due to the small sample size, this initial strain-specific association could suggest that genetic factors may interact with particular <i>M. tuberculosis</i> strains to influence disease severity.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19576"},"PeriodicalIF":2.4000,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145778/pdf/","citationCount":"0","resultStr":"{\"title\":\"Interferon-gamma and interleukin-10 genetic polymorphisms among the pulmonary tuberculosis caused by Malaysia-specific <i>Mycobacterium tuberculosis</i> strain: SIT745/EAI1-MYS.\",\"authors\":\"Nik Mohd Noor Nik Zuraina, Nur Annisa Nasuha Mohd Sedi, Mohammed Dauda Goni, Suharni Mohamad, Mohd Nor Norazmi, Siti Suraiya\",\"doi\":\"10.7717/peerj.19576\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong><i>Mycobacterium tuberculosis</i> is the causative agent of tuberculosis, which results in 1.5 million global deaths annually. Key factors influencing tuberculosis development encompass host genetic factors, genetic diversity within <i>M. tuberculosis</i>, and environmental conditions. The East-African-Indian (EAI) lineage is the predominant lineage in Southeast Asia, India, and East Africa. A recent finding has identified SIT745/EAI1-MYS, a sub-lineage of EAI, as the geographically specific <i>M. tuberculosis</i> strain in Malaysia.</p><p><strong>Methods: </strong>This study aimed to investigate the role of IFN-γ (+874) A/T and IL-10 (-1,082) A/G SNPs with tuberculosis (TB) disease susceptibility among patients infected with <i>M. tuberculosis</i> SIT745/EAI1-MYS strain. A cross-sectional study was conducted between three groups of subjects consisted of TB patients infected with <i>M. tuberculosis</i> SIT745/EAI1-MYS strain (<i>n</i> = 9), TB patients of non-<i>M. tuberculosis</i> SIT745/EAI1-MYS strains (<i>n</i> = 9), and healthy controls (<i>n</i> = 9). The genetic variation in IFN-γ (+874) A/T and IL-10 (-1,082) A/G SNPs were detected using allele-specific PCR and analyzed for their association with TB risk and severity.</p><p><strong>Results: </strong>The results indicated a higher frequency of the IFN-γ (+874) TT and IL-10 (-1,082) AG genotypes among TB patients compared to healthy controls. The IFN-γ (+874) AA and IL-10 (-1,082) AG genotypes were more prevalent among TB patients infected with the SIT745/EAI1-MYS <i>M. tuberculosis</i> strains compared to non-SIT745/EAI1-MYS, indicating a possible link between these genotypes and more severe TB symptoms. Although there is no significant correlation between the IFN-γ (+874) A/T and IL-10 (-1,082) A/G polymorphisms with the susceptibility or severity of TB due to the small sample size, this initial strain-specific association could suggest that genetic factors may interact with particular <i>M. tuberculosis</i> strains to influence disease severity.</p>\",\"PeriodicalId\":19799,\"journal\":{\"name\":\"PeerJ\",\"volume\":\"13 \",\"pages\":\"e19576\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-06-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145778/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PeerJ\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.7717/peerj.19576\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PeerJ","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.7717/peerj.19576","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Interferon-gamma and interleukin-10 genetic polymorphisms among the pulmonary tuberculosis caused by Malaysia-specific Mycobacterium tuberculosis strain: SIT745/EAI1-MYS.
Background: Mycobacterium tuberculosis is the causative agent of tuberculosis, which results in 1.5 million global deaths annually. Key factors influencing tuberculosis development encompass host genetic factors, genetic diversity within M. tuberculosis, and environmental conditions. The East-African-Indian (EAI) lineage is the predominant lineage in Southeast Asia, India, and East Africa. A recent finding has identified SIT745/EAI1-MYS, a sub-lineage of EAI, as the geographically specific M. tuberculosis strain in Malaysia.
Methods: This study aimed to investigate the role of IFN-γ (+874) A/T and IL-10 (-1,082) A/G SNPs with tuberculosis (TB) disease susceptibility among patients infected with M. tuberculosis SIT745/EAI1-MYS strain. A cross-sectional study was conducted between three groups of subjects consisted of TB patients infected with M. tuberculosis SIT745/EAI1-MYS strain (n = 9), TB patients of non-M. tuberculosis SIT745/EAI1-MYS strains (n = 9), and healthy controls (n = 9). The genetic variation in IFN-γ (+874) A/T and IL-10 (-1,082) A/G SNPs were detected using allele-specific PCR and analyzed for their association with TB risk and severity.
Results: The results indicated a higher frequency of the IFN-γ (+874) TT and IL-10 (-1,082) AG genotypes among TB patients compared to healthy controls. The IFN-γ (+874) AA and IL-10 (-1,082) AG genotypes were more prevalent among TB patients infected with the SIT745/EAI1-MYS M. tuberculosis strains compared to non-SIT745/EAI1-MYS, indicating a possible link between these genotypes and more severe TB symptoms. Although there is no significant correlation between the IFN-γ (+874) A/T and IL-10 (-1,082) A/G polymorphisms with the susceptibility or severity of TB due to the small sample size, this initial strain-specific association could suggest that genetic factors may interact with particular M. tuberculosis strains to influence disease severity.
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