新一代基于纳米膜的多价候选疫苗,以应对日益严重的m痘威胁。

IF 6.9 1区 医学 Q1 IMMUNOLOGY
Rahul Ahuja, Preeti Vishwakarma, Varun Kumar, Ritika Khatri, Ananya Chatterjee, Surbhi Mishra, Zaigham Abbas Rizvi, Anup Singh, Gurleen Kaur, Vikas Maithil, Kunal Tarane, Akanksha Chauhan, Sarjeet Singh, Pooja Yadav, Devendra Yadav, Sangita Kumari Sinha, Syed Khalid Ali, Abhisek Chatterjee, Priyanka Priyadarsiny, Amit Awasthi, Vidya Mangala Prasad, Shubbir Ahmed, Sweety Samal
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引用次数: 0

摘要

最近人类猴痘病毒(MPXV)的出现和全球传播,包括其向非流行地区的传播,引起了重大的全球卫生关切。在这项概念验证研究中,我们开发了一种基于重组蛋白的MPXV候选疫苗,采用了一种创新的、多价的、自组装的纳米笼蛋白支架。来自当代循环MPXV菌株的两种免疫原性抗原已被纳入自组装非结构蛋白-10 (NSP-10)支架中,使用无纯化标签的大肠杆菌表达系统进行表达和纯化。该候选疫苗在小鼠中引发了强烈的抗体反应,并在鼻内和皮肤袋体内研究中对致命的牛痘病毒具有保护作用。此外,在免疫小鼠中使用MPXV株进化枝IIb的鼻内攻击显示出有希望的结果,包括病毒滴度显着降低并引发强大的中和抗体反应。这项研究为开发MPXV疫苗提供了一种可行的、可扩展的和具有成本效益的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Next-gen novel nanocage-based multivalent vaccine candidate to tackle the rising menace of Mpox.

The recent emergence and global spread of the human Monkeypox virus (MPXV), including its transmission to non-endemic regions, have raised significant global health concerns. In this proof-of-concept study, we developed a recombinant protein-based MPXV vaccine candidate, employing an innovative and versatile multivalent, self-assembled nanocage protein scaffold. Two immunogenic antigens derived from the contemporary circulating MPXV strain have been incorporated into a self-assembled non-structural protein-10 (NSP-10) scaffold, expressed, and purified using an Escherichia coli expression system without a purification tag. The vaccine candidate elicited strong antibody responses in mice and conferred protection against the lethal Vaccinia virus in an intranasal and skin pock in vivo study. Additionally, an intranasal challenge with the MPXV strain clade IIb in immunized mice demonstrated promising outcomes, including a significant reduction in viral titres and eliciting a robust neutralizing antibody response. This study demonstrates a feasible, scalable, and cost-effective approach for the development of the MPXV vaccine.

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来源期刊
NPJ Vaccines
NPJ Vaccines Immunology and Microbiology-Immunology
CiteScore
11.90
自引率
4.30%
发文量
146
审稿时长
11 weeks
期刊介绍: Online-only and open access, npj Vaccines is dedicated to highlighting the most important scientific advances in vaccine research and development.
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