在超重、肥胖和代谢综合征的胰岛素抵抗个体中,粪便消化不良杆菌的浓度与血浆脂质组有关。

IF 3.9 2区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Camille Petitfils, Clara Depommier, Nathalie M Delzenne, Amandine Everard, Matthias Van Hul, Patrice D Cani
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引用次数: 0

摘要

背景:肥胖在世界范围内达到流行病的程度。脂肪组织的过度增加是多种疾病发展和过早死亡的危险因素。在相关疾病中,代谢综合征是肥胖的主要合并症之一。在这种情况下,肠道微生物群被认为既塑造宿主的能量代谢,又对宿主的能量代谢做出反应。最近,代谢组学已经成为捕获特定组织中代谢物快照的强大工具,为宿主-微生物群相互作用提供了新的见解。将代谢组学与肠道微生物群研究相结合可以帮助我们更好地了解特定物种如何影响宿主代谢组学特征。威氏消化不良杆菌(Dysosmobacter welbionis)已被确定为下一代有益细菌,对小鼠的脂肪质量和葡萄糖代谢有潜在影响,粪便中消化不良杆菌的浓度与人体体重指数、空腹血糖和血浆HbA1c呈负相关。方法:采用qPCR方法对伴有代谢综合征的胰岛素抵抗型超重/肥胖受试者的粪便中消化不良杆菌的浓度进行定量分析,并采用非靶向代谢组学方法对血浆代谢物进行分析。利用Spearman相关性发现了厌氧菌粪便丰度与1169种已鉴定血浆代谢物之间的相关性,随后进行了错误发现率校正。结果:有趣的是,在检测到的代谢物中,Dysosmobacter sp只与脂质分子相关。厌氧杆菌的粪便浓度与血浆中5种磷脂酰胆碱、花生四烯酸、2种单酰基甘油、12种二酰基甘油、3种溶血磷脂酰乙醇胺、1种磷脂酰肌醇和3种溶血磷脂酰肌醇以及甘油乙醇胺、甘油磷脂酰胆碱和PC的水平呈正相关(p值为16:0)。与酰胆碱和溶血磷脂酰胆碱代谢物的相关性特别有趣,分别有6/8和8/10检测到的分子与消化不良杆菌属正相关。结论:这些结果表明,消化不良杆菌属在宿主脂质代谢中起特定作用。这一发现与先前的体内研究一致,强调了用这种细菌治疗的小鼠多种组织的脂质谱改变。需要进一步的研究来阐明其潜在的机制并评估其潜在的治疗应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fecal Dysosmobacter spp. concentration is linked to plasma lipidome in insulin-resistant individuals with overweight, obesity and metabolic syndrome.

Background: Obesity is reaching epidemic proportions worldwide. This excessive increase of adipose tissue is a risk factor for the development of multiple diseases and premature death. Amongst associated diseases, metabolic syndrome is one of the main comorbidities of obesity. In this context, the gut microbiota has been recognized as both shaping and responding to host energy metabolism. Recently metabolomics has emerged as a powerful tool to capture a snapshot of the metabolites present in a specific tissue, providing new insights into host-microbiota interactions. Integrating metabolomics with gut microbiota studies could help us better understand how specific species impact on host metabolomic profile. Dysosmobacter welbionis has been identified as a promising next generation beneficial bacteria with potential effects on fat mass and glucose metabolism in mice, and fecal Dysosmobacter spp. concentration was inversely correlated to body mass index fasting glucose and plasmatic HbA1c in humans.

Methods: Concentration of Dysosmobacter spp. was quantified by qPCR in the stools of insulin resistant overweight/obese participants with a metabolic syndrome and plasma metabolites were analyzed using untargeted metabolomics. Correlations between Dysosmobacter spp. fecal abundance and the 1169 identified plasma metabolites were uncovered using Spearman correlations followed by a false discovery rate correction.

Results: Interestingly, among the detected metabolites, Dysosmobacter spp. was exclusively associated with lipid molecules. Fecal concentration of Dysosmobacter spp. was positively associated with plasmatic levels of five phosphatidylcholines, arachidonate, two monoacylglycerols, twelve diacylglycerols, three lysophosphatidylethanolamines, one phosphatidylinositol and three lysophosphatidylinositols, as well as glycerophosphoethanolamines, glycerophosphatidylcholine and PC(P-16:0). The correlation was particularly interesting with acylcholine and lysophosphatidylcholine metabolites as, respectively, 6/8 and 8/10 detected molecules were positively associated with Dysosmobacter spp.

Conclusion: These results suggest that Dysosmobacter spp. plays a specific role in host lipid metabolism. This finding aligns with previous in vivo studies highlighting lipid profile alterations in multiple tissues of mice treated with this bacterium. Further studies are needed to elucidate the underlying mechanisms and assess its potential therapeutic applications.

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来源期刊
Lipids in Health and Disease
Lipids in Health and Disease 生物-生化与分子生物学
CiteScore
7.70
自引率
2.20%
发文量
122
审稿时长
3-8 weeks
期刊介绍: Lipids in Health and Disease is an open access, peer-reviewed, journal that publishes articles on all aspects of lipids: their biochemistry, pharmacology, toxicology, role in health and disease, and the synthesis of new lipid compounds. Lipids in Health and Disease is aimed at all scientists, health professionals and physicians interested in the area of lipids. Lipids are defined here in their broadest sense, to include: cholesterol, essential fatty acids, saturated fatty acids, phospholipids, inositol lipids, second messenger lipids, enzymes and synthetic machinery that is involved in the metabolism of various lipids in the cells and tissues, and also various aspects of lipid transport, etc. In addition, the journal also publishes research that investigates and defines the role of lipids in various physiological processes, pathology and disease. In particular, the journal aims to bridge the gap between the bench and the clinic by publishing articles that are particularly relevant to human diseases and the role of lipids in the management of various diseases.
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