大麻叶提取物对抗胆管癌活性的影响

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine Pub Date : 2025-05-15 DOI:10.1016/j.hermed.2025.101028

Pranee Sriraj , Thidarut Boonmars , Areeya Changthong , Praphat Manuelo Ruengthanoo , Panicha Pongnaratorn , Ratchadawan Aukkanimart

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引用次数: 0

摘要

本研究评估鼠尾大麻（CLE）抑制胆管癌细胞(CCA；KKU213B)。方法采用Folin-Ciocalteu试剂法测定总酚含量。本研究还试图比较鼠尾大麻与没食子酸的药效，用AlCl3比色法评价鼠尾大麻的总黄酮含量，并与鼠尾大麻与槲皮素的总黄酮含量进行比较。通过DPPH和ABTS测定抗氧化活性。利用高效液相色谱法对CLE中的关键化合物进行了鉴定。采用硫代磺胺B法评价其对胆管癌细胞生长的抑制作用，通过分析凋亡相关蛋白的表达来探讨其凋亡机制。结果研究发现，白芷总酚含量与抗氧化活性呈正相关。高效液相色谱分析表明，CLE中大麻酚含量为89.57 ± 10.34 µg/mg。对KKU-213B细胞的生长抑制实验表明，大麻酚能有效抑制细胞生长，IC50值分别为8.14 ± 0.10、7.30 ± 0.38和4.00 ± 0.96 µM（24、48和72 小时）。CLE抑制KKU-213B细胞生长的IC50值在24、48和72 小时分别为55.26 ± 1.64、42.25 ± 5.56和18.02 ± 9.43 µg/ml，与对照组有显著差异。关于细胞凋亡的机制，CLE可刺激p53、caspase-3和Bax的表达，同时降低Bcl-2的表达。结论Sakon Nakhon菌株的scle可能是一种潜在的抗癌药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Impact of Cannabis Leaf (Sakon Nakhon Cultivar) Extracts on Anticholangiocarcinoma Activity

Introduction

This study assesses the efficacy of Squirrel Tail cannabis (CLE) in inhibiting cholangiocarcinoma cells (CCA; KKU213B).

Methods

The total phenolic content was quantified using the Folin-Ciocalteu reagent method. The study also sought to compare the efficacy of Squirrel Tail cannabis with gallic acid and evaluate the total flavonoid content using the AlCl₃ colorimetric method, compared with the total flavonoid content of CLE with quercetin. Antioxidant activity was assessed through DPPH and ABTS assays. Key compounds in CLE were identified using High-performance liquid chromatography. Inhibition of cholangiocarcinoma cell growth was evaluated using the sulforhodamine B assay, and the mechanism of apoptosis was examined by analysing the expression of apoptosis-related proteins.

Results

The study found that the total phenolic content of CLE correlated with antioxidant activities. High-performance liquid chromatography analysis revealed that CLE contained 89.57 ± 10.34 µg/mg of cannabinol. Growth inhibition assays on KKU-213B cells indicated that cannabinol effectively inhibited cell growth, with IC₅₀ values of 8.14 ± 0.10, 7.30 ± 0.38, and 4.00 ± 0.96 µM (24, 48, and 72 hours). CLE demonstrated the ability to inhibit KKU-213B cell growth with IC₅₀ values of 55.26 ± 1.64, 42.25 ± 5.56, and 18.02 ± 9.43 µg/ml at 24, 48, and 72 hours, respectively, which were significantly different from those of the control group. Regarding the mechanism of apoptosis, CLE was found to stimulate the expression of p53, caspase-3, and Bax while reducing the expression of Bcl-2.

Conclusions

CLE from Sakon Nakhon strains may serve as a potential alternative for anticancer therapies.

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来源期刊

Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.