比率近红外探针的从头设计，揭示铁下垂和自噬过程中线粒体内膜的相变

IF 4.1 3区工程技术 Q2 CHEMISTRY, APPLIED

Dyes and Pigments Pub Date : 2025-06-06 DOI:10.1016/j.dyepig.2025.112948

Chenyu Shi , Zhiyuan Wang , Qingqing Lu , Minggang Tian

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引用次数: 0

摘要

线粒体膜上的液体有序（Lo）相和液体无序（Ld）相微结构域在代谢、信号传导和细胞凋亡等重要生物活动中发挥着不可或缺但又不同的作用。因此，辨别线粒体膜上的Lo和Ld相是基础生物学和病理学的一项紧迫任务。然而，用于线粒体膜相比例可视化的荧光探针很少被报道。在此，我们通过调节电子供体和受体构建了一种对环境极性高度敏感的双发射氟化物，并重新设计了一种荧光探针（MT-phs），用于线粒体内膜中Lo和Ld微结构域的分离和比例可视化。模型膜和脂质处理实验证明，MT-phs能够以比例法区分Lo和Ld相。利用新设计的探针，揭示了铁下垂和饥饿刺激时线粒体内膜Ld结构域和流动性的增加。对线粒体的相变过程也进行了现场实时监测。

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De novo design of ratiometric near-infrared probe revealing phase transition of mitochondrial inner membrane during ferroptosis and autophagy

The liquid ordered (L_o) and liquid disordered (L_d) phase microdomains in mitochondrial inner membranes play indispensable yet different roles in vital bioactivities including metabolism, signaling, and apoptosis. Therefore, discriminatively visualizing L_o and L_d phases in mitochondrial membranes is an urgent task for fundamental biology and pathology. However, fluorescent probes for ratiometric visualization of the membrane phases in mitochondria are rarely reported. Herein, we have constructed a dual-emissive fluorogen highly sensitive to environmental polarity by regulating the electron donor and acceptor, and de novo designed a fluorescent probe (MT-phs) for the separate and ratiometric visualization of L_o and L_d microdomains in mitochondrial inner membranes. Model membrane and lipids treatment experiments proved that MT-phs enabled the discrimination of L_o and L_d phase in ratiometric manner. With the newly designed probe, the increased L_d domains and fluidity in mitochondrial inner membranes during ferroptosis and starvation stimuli has been revealed. The phase transition in mitochondria were also in-situ and real-timely monitored successfully.

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.