发布求助

文献互助智能选刊最新文献

建立微球流式细胞术定量检测总免疫球蛋白E的方法

IF 3.2 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinica Chimica Acta Pub Date : 2025-06-04 DOI:10.1016/j.cca.2025.120415

Qi Jiang , Yuanmin Sun , Xiaoming Cui , Yixian Li , Xiaohui Yang , Xue Li , Huiqiang Li , Yang Yu

{"title":"建立微球流式细胞术定量检测总免疫球蛋白E的方法","authors":"Qi Jiang , Yuanmin Sun , Xiaoming Cui , Yixian Li , Xiaohui Yang , Xue Li , Huiqiang Li , Yang Yu","doi":"10.1016/j.cca.2025.120415","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>In the diagnosis of allergic diseases, in vitro serum IgE level tests were conventionally used. In recent years, microsphere-based flow cytometric assays for IgE have gained more attention. The present study aimed to establish a microsphere-based flow cytometric assay for quantitative detection of tIgE.</div></div><div><h3>Methods</h3><div>The assay was established after selection of microsphere, antibody pair, detection mode, and optimization of coating ratio. In order to quantitate tIgE, the calibration curve was established. The detection performance of this new tIgE flow cytometric assay was also confirmed. In addition, the correlations between this assay with the ImmunoCAP assay or the immunoturbidimetry assay were evaluated.</div></div><div><h3>Results</h3><div>This new tIgE flow cytometric assay has low CVs of repeatability (7.62 % and 6.98 %) and intermediate precision (7.50 % and 8.41 %). The detection range was 2 ∼ 6000 IU/mL. The range of linearity was from 2 ∼ 3363 IU/L (<em>r</em> = 0.9932). The correlation coefficient (r) for the correlation analysis between the results of the newly developed tIgE flow cytometric assay and ImmunoCAP assay or immunoturbidimetry assay was 0.9345 and 0.9348, respectively.</div></div><div><h3>Conclusion</h3><div>A tIgE flow cytometric assay was successfully established. It could be used in clinical laboratories and quantitate lots of allergen-sIgE detected by flow cytometric assay.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"576 ","pages":"Article 120415"},"PeriodicalIF":3.2000,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Establishment of a microsphere-based flow cytometric assay for quantitative detection of total immunoglobulin E\",\"authors\":\"Qi Jiang , Yuanmin Sun , Xiaoming Cui , Yixian Li , Xiaohui Yang , Xue Li , Huiqiang Li , Yang Yu\",\"doi\":\"10.1016/j.cca.2025.120415\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>In the diagnosis of allergic diseases, in vitro serum IgE level tests were conventionally used. In recent years, microsphere-based flow cytometric assays for IgE have gained more attention. The present study aimed to establish a microsphere-based flow cytometric assay for quantitative detection of tIgE.</div></div><div><h3>Methods</h3><div>The assay was established after selection of microsphere, antibody pair, detection mode, and optimization of coating ratio. In order to quantitate tIgE, the calibration curve was established. The detection performance of this new tIgE flow cytometric assay was also confirmed. In addition, the correlations between this assay with the ImmunoCAP assay or the immunoturbidimetry assay were evaluated.</div></div><div><h3>Results</h3><div>This new tIgE flow cytometric assay has low CVs of repeatability (7.62 % and 6.98 %) and intermediate precision (7.50 % and 8.41 %). The detection range was 2 ∼ 6000 IU/mL. The range of linearity was from 2 ∼ 3363 IU/L (<em>r</em> = 0.9932). The correlation coefficient (r) for the correlation analysis between the results of the newly developed tIgE flow cytometric assay and ImmunoCAP assay or immunoturbidimetry assay was 0.9345 and 0.9348, respectively.</div></div><div><h3>Conclusion</h3><div>A tIgE flow cytometric assay was successfully established. It could be used in clinical laboratories and quantitate lots of allergen-sIgE detected by flow cytometric assay.</div></div>\",\"PeriodicalId\":10205,\"journal\":{\"name\":\"Clinica Chimica Acta\",\"volume\":\"576 \",\"pages\":\"Article 120415\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-06-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinica Chimica Acta\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0009898125002943\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinica Chimica Acta","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0009898125002943","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

背景在变态反应性疾病的诊断中，常规采用体外血清IgE水平检测。近年来，基于微球的流式细胞术检测IgE得到了越来越多的关注。本研究旨在建立一种基于微球的流式细胞术定量检测tIgE的方法。方法选择微球、抗体对、检测方式、优化包被比，建立检测方法。为了定量测定tIgE，建立了tIgE的标定曲线。这种新的tIgE流式细胞分析方法的检测性能也得到了证实。此外，还评估了该检测与免疫cap检测或免疫比浊法检测之间的相关性。结果该方法重复性CVs值低（7.62%和6.98%），精密度中等（7.50%和8.41%）。检测范围为2 ~ 6000 IU/mL。线性范围为2 ~ 3363 IU/L （r = 0.9932）。新建立的tIgE流式细胞分析结果与ImmunoCAP、免疫比浊法的相关系数r分别为0.9345、0.9348。结论成功建立了tIgE流式细胞术检测方法。该方法可用于临床实验室，对流式细胞术检测到的大量过敏原sige进行定量分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Establishment of a microsphere-based flow cytometric assay for quantitative detection of total immunoglobulin E

Background

In the diagnosis of allergic diseases, in vitro serum IgE level tests were conventionally used. In recent years, microsphere-based flow cytometric assays for IgE have gained more attention. The present study aimed to establish a microsphere-based flow cytometric assay for quantitative detection of tIgE.

Methods

The assay was established after selection of microsphere, antibody pair, detection mode, and optimization of coating ratio. In order to quantitate tIgE, the calibration curve was established. The detection performance of this new tIgE flow cytometric assay was also confirmed. In addition, the correlations between this assay with the ImmunoCAP assay or the immunoturbidimetry assay were evaluated.

Results

This new tIgE flow cytometric assay has low CVs of repeatability (7.62 % and 6.98 %) and intermediate precision (7.50 % and 8.41 %). The detection range was 2 ∼ 6000 IU/mL. The range of linearity was from 2 ∼ 3363 IU/L (r = 0.9932). The correlation coefficient (r) for the correlation analysis between the results of the newly developed tIgE flow cytometric assay and ImmunoCAP assay or immunoturbidimetry assay was 0.9345 and 0.9348, respectively.

Conclusion

A tIgE flow cytometric assay was successfully established. It could be used in clinical laboratories and quantitate lots of allergen-sIgE detected by flow cytometric assay.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Clinica Chimica Acta

Clinica Chimica Acta 医学-医学实验技术

CiteScore

10.10

自引率

2.00%

发文量

1268

审稿时长

23 days

期刊介绍： The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells. The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.

联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1

京公网安备 11010802042870号

Book学术文献互助

Book学术文献互助群
群号：481959085

Book学术官方微信