商业储蓄酶对消费级PLA的有效降解:优化条件和分子动力学见解

IF 7.3 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Marija Nenadović, Marijana Ponjavić, Brana Pantelic, Maciej Guzik, Tomasz M. Majka, Georgia Sourkouni, Aleksandra Maršavelski, Jasmina Nikodinovic-Runic
{"title":"商业储蓄酶对消费级PLA的有效降解:优化条件和分子动力学见解","authors":"Marija Nenadović, Marijana Ponjavić, Brana Pantelic, Maciej Guzik, Tomasz M. Majka, Georgia Sourkouni, Aleksandra Maršavelski, Jasmina Nikodinovic-Runic","doi":"10.1021/acssuschemeng.5c03378","DOIUrl":null,"url":null,"abstract":"Poly(lactic acid) (PLA) is the most widely produced biopolymer globally, with 920,000 tons reaching the market in 2024. Although it is a biobased polymer and biodegradable, it currently poses a threat by generating a considerable waste stream in the environment unless its end-of-life options are further developed. Biorecycling of PLA is a promising solution and is critical for PLA to be a truly sustainable alternative to conventional plastics, further enabling the (bio)plastics circular economy. This work investigates the repurposing of commercial Savinase 12T preparation, already produced on a large scale for the detergent industry, for the degradation of consumer-grade PLA. Savinase-degraded postconsumer PLA single-use cups at a rate of 166 mg·day<sup>–1</sup>·mg enzyme<sup>–1</sup> for the lid part (crystallinity (<i>X</i><sub>c</sub>) 22.9%) and 40 mg·day<sup>–1</sup>·mg enzyme<sup>–1</sup> for the body part (<i>X</i><sub>c</sub> 42.0%). The average degradation rate of PLA 3D printer filaments was determined to be 56 mg·day<sup>–1</sup>·mg enzyme<sup>–1</sup> under optimized conditions of pH 8.5 and 42 °C. Lactic acid was identified as the main PLA degradation product of Savinase depolymerizing activity. Molecular modeling revealed Asn155 and Ser125 as key residues involved in PLA tetramer binding.","PeriodicalId":25,"journal":{"name":"ACS Sustainable Chemistry & Engineering","volume":"35 1 1","pages":""},"PeriodicalIF":7.3000,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Efficient Degradation of Consumer-Grade PLA by Commercial Savinase: Optimized Conditions and Molecular Dynamics Insights\",\"authors\":\"Marija Nenadović, Marijana Ponjavić, Brana Pantelic, Maciej Guzik, Tomasz M. Majka, Georgia Sourkouni, Aleksandra Maršavelski, Jasmina Nikodinovic-Runic\",\"doi\":\"10.1021/acssuschemeng.5c03378\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Poly(lactic acid) (PLA) is the most widely produced biopolymer globally, with 920,000 tons reaching the market in 2024. Although it is a biobased polymer and biodegradable, it currently poses a threat by generating a considerable waste stream in the environment unless its end-of-life options are further developed. Biorecycling of PLA is a promising solution and is critical for PLA to be a truly sustainable alternative to conventional plastics, further enabling the (bio)plastics circular economy. This work investigates the repurposing of commercial Savinase 12T preparation, already produced on a large scale for the detergent industry, for the degradation of consumer-grade PLA. Savinase-degraded postconsumer PLA single-use cups at a rate of 166 mg·day<sup>–1</sup>·mg enzyme<sup>–1</sup> for the lid part (crystallinity (<i>X</i><sub>c</sub>) 22.9%) and 40 mg·day<sup>–1</sup>·mg enzyme<sup>–1</sup> for the body part (<i>X</i><sub>c</sub> 42.0%). The average degradation rate of PLA 3D printer filaments was determined to be 56 mg·day<sup>–1</sup>·mg enzyme<sup>–1</sup> under optimized conditions of pH 8.5 and 42 °C. Lactic acid was identified as the main PLA degradation product of Savinase depolymerizing activity. Molecular modeling revealed Asn155 and Ser125 as key residues involved in PLA tetramer binding.\",\"PeriodicalId\":25,\"journal\":{\"name\":\"ACS Sustainable Chemistry & Engineering\",\"volume\":\"35 1 1\",\"pages\":\"\"},\"PeriodicalIF\":7.3000,\"publicationDate\":\"2025-06-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Sustainable Chemistry & Engineering\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1021/acssuschemeng.5c03378\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Sustainable Chemistry & Engineering","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acssuschemeng.5c03378","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

聚乳酸(PLA)是全球生产最广泛的生物聚合物,2024年将有92万吨进入市场。虽然它是一种生物基聚合物,可生物降解,但目前它对环境构成了威胁,除非进一步开发其报废选择。聚乳酸的生物回收是一个很有前途的解决方案,对于聚乳酸成为传统塑料的真正可持续替代品至关重要,进一步实现(生物)塑料循环经济。这项工作调查了商业Savinase 12T制剂的再利用,已经为洗涤剂工业大规模生产,用于降解消费级PLA。savinase降解PLA一次性杯,杯盖部分酶解率为166 mg·day-1·mg酶- 1(结晶度(Xc) 22.9%),杯体部分酶解率为40 mg·day-1·mg酶- 1 (Xc) 42.0%)。在pH 8.5和42°C的优化条件下,PLA 3D打印长丝的平均降解率为56 mg·d - 1·mg酶- 1。乳酸被确定为具有Savinase解聚活性的主要PLA降解产物。分子模型显示Asn155和Ser125是参与PLA四聚体结合的关键残基。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Efficient Degradation of Consumer-Grade PLA by Commercial Savinase: Optimized Conditions and Molecular Dynamics Insights
Poly(lactic acid) (PLA) is the most widely produced biopolymer globally, with 920,000 tons reaching the market in 2024. Although it is a biobased polymer and biodegradable, it currently poses a threat by generating a considerable waste stream in the environment unless its end-of-life options are further developed. Biorecycling of PLA is a promising solution and is critical for PLA to be a truly sustainable alternative to conventional plastics, further enabling the (bio)plastics circular economy. This work investigates the repurposing of commercial Savinase 12T preparation, already produced on a large scale for the detergent industry, for the degradation of consumer-grade PLA. Savinase-degraded postconsumer PLA single-use cups at a rate of 166 mg·day–1·mg enzyme–1 for the lid part (crystallinity (Xc) 22.9%) and 40 mg·day–1·mg enzyme–1 for the body part (Xc 42.0%). The average degradation rate of PLA 3D printer filaments was determined to be 56 mg·day–1·mg enzyme–1 under optimized conditions of pH 8.5 and 42 °C. Lactic acid was identified as the main PLA degradation product of Savinase depolymerizing activity. Molecular modeling revealed Asn155 and Ser125 as key residues involved in PLA tetramer binding.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
ACS Sustainable Chemistry & Engineering
ACS Sustainable Chemistry & Engineering CHEMISTRY, MULTIDISCIPLINARY-ENGINEERING, CHEMICAL
CiteScore
13.80
自引率
4.80%
发文量
1470
审稿时长
1.7 months
期刊介绍: ACS Sustainable Chemistry & Engineering is a prestigious weekly peer-reviewed scientific journal published by the American Chemical Society. Dedicated to advancing the principles of green chemistry and green engineering, it covers a wide array of research topics including green chemistry, green engineering, biomass, alternative energy, and life cycle assessment. The journal welcomes submissions in various formats, including Letters, Articles, Features, and Perspectives (Reviews), that address the challenges of sustainability in the chemical enterprise and contribute to the advancement of sustainable practices. Join us in shaping the future of sustainable chemistry and engineering.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信