培养难度:相对于PCR,使用更多的标本来增加耐甲氧西林金黄色葡萄球菌的培养产量。

Access microbiology Pub Date : 2025-06-03 eCollection Date: 2025-01-01 DOI:10.1099/acmi.0.000918.v4
Arvette E Mitchell, Arpit P Patel, Jennifer DiCandilo, Zachary W Rebollido, Matthew A Pettengill
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引用次数: 0

摘要

耐甲氧西林金黄色葡萄球菌(MRSA)在社区获得性感染和医疗保健相关感染中引起相当大的发病率和死亡率,但在某些临床环境中,检测MRSA定植已被证明可以改善患者的预后。MRSA定植检测的方法多种多样,相对于培养,分子测定法在大多数研究中具有更高的灵敏度,但培养法在某些比较中由于利用低标本体积而处于劣势。我们将商业分子检测与小体积(10µl)和大体积(650µl)培养进行了比较,发现增加用于培养的体积比小体积培养多检测25%的病例。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Culture harder: use more specimens to increase methicillin-resistant Staphylococcus aureus culture yield relative to PCR.

Methicillin-resistant Staphylococcus aureus (MRSA) causes considerable morbidity and mortality in both community-acquired and healthcare-associated infections, but detecting colonization with MRSA has been shown to improve patient outcomes in certain clinical settings. MRSA colonization detection has been carried out in a variety of ways, with molecular assays having superior sensitivity in most studies relative to culture, but culture is disadvantaged in some comparisons by utilization of low specimen volumes. We compared a commercial molecular assay to both low-volume (10 µl) and high-volume (650 µl) cultures and found that increasing the volume utilized for culture led to the detection of 25% more cases than low-volume culture.

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