无瓣荆芥叶提取物的体外抗菌、DPPH自由基清除活性及体外实验研究。

IF 1.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Tolessa Duguma, Yadessa Melaku, Akalu Terfa, Kebede Shenkute, Taye B Demissie, Nayang Kgakatsi, Mo Hunsen
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引用次数: 0

摘要

无瓣红桃。是埃塞俄比亚的一种药用植物,用于治疗不同的疾病。本研究旨在通过体外和计算机实验,从紫花苜蓿叶片中分离植物化学物质,并评价其抗菌和清除DPPH的活性。GC-MS共鉴定出16个化合物。利用色谱技术分离棕榈酸(9)、棕榈醇(17)、β-谷甾醇(18)、tiliroside(19)和黄芪苷(20),并用NMR数据对其进行了表征。100 mg/mL提取物和5 mg/mL分离化合物对所有细菌的抑制区(IZs)范围为9.33±0.47 ~ 15.07±0.74 mm。化合物18对金黄色葡萄球菌和化脓性链球菌有较好的抑制作用。化合物19对大肠杆菌和铜绿假单胞菌具有较好的抑制作用。化合物19对大肠杆菌DNA旋切酶B(-8.0 kcal/mol)和铜绿假单胞菌喹诺酮假单胞菌信号A (PqsA, -9.5 kcal/mol)的活性得到了分子对接研究的支持。当浓度为62.5 mg/mL时,化合物16-21和化合物19的DPPH清除率分别最低(54.5 %)和最高(89.8 %)。计算机研究表明,如果吞下这些分离出来的化合物,没有一种是致命的。因此,这些发现支持了用这种植物治疗各种细菌性疾病的传统做法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro antibacterial, DPPH radical scavenging activities, and in silico studies of isolated compounds from Rubus apetalus leaves extract.

Rubus apetalus Poir. is a medicinal plant used in Ethiopia to treat different diseases. This study aimed to isolate phytochemicals from the leaves and evaluate their antibacterial and DPPH scavenging activities using in vitro and in silico studies. The GC-MS study resulted in the identification of 16 compounds. Palmitic acid (9), palmityl alcohol (17), β-sitosterol (18), tiliroside (19), and astragalin (20) were isolated using chromatographic techniques and characterized using NMR data. The inhibition zones (IZs) of 100 mg/mL extracts and 5 mg/mL isolated compounds ranged from 9.33 ± 0.47 to 15.07 ± 0.74 mm against all bacteria. Compound 18 showed better activity against Staphylococcus aureus and Streptococcus pyogenes. Compound 19 exhibited better activity against Escherichia coli and Pseudomonas aeruginosa. The activity of compound 19 was supported by molecular docking studies against E. coli DNA gyrase B (-8.0 kcal/mol) and Pseudomonas quinolone signal A (PqsA, -9.5 kcal/mol) for E. coli and P. aeruginosa, respectively. At 62.5 mg/mL, combined fractions 16-21 and compound 19 showed the lowest (54.5 %) and the highest (89.8 %) DPPH scavenging activity, respectively. The in silico studies indicated that none of the isolated compounds is fatal if swallowed. Therefore, these findings support the traditional use of the plant to treat various bacterial diseases.

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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
55
期刊介绍: A Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC) is an international scientific journal and a community resource for the emerging field of natural and natural-like products. The journal publishes original research on the isolation (including structure elucidation), bio-chemical synthesis and bioactivities of natural products, their biochemistry, pharmacology, biotechnology, and their biological activity and innovative developed computational methods for predicting the structure and/or function of natural products. A Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC) welcomes research papers in fields on the chemistry-biology boundary which address scientific ideas and approaches to generate and understand natural compounds on a molecular level and/or use them to stimulate and manipulate biological processes.
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