乳酸菌生产的无细胞上清液对禽肉安全和品质的影响。

IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
B Ozturk, I Y Sengun
{"title":"乳酸菌生产的无细胞上清液对禽肉安全和品质的影响。","authors":"B Ozturk, I Y Sengun","doi":"10.1080/00071668.2025.2500344","DOIUrl":null,"url":null,"abstract":"<p><p>1. This research characterised cell-free supernatants (CFS) obtained from lactic acid bacteria (<i>Leuconostoc pseudomesenteroides</i> T139, <i>Pediococcus acidilactici</i> T115 and <i>Streptococcus thermophilus</i> T127) and examined the effects of CFS (10%) on the safety and quality of poultry meat stored at 4°C for 7 d.2. In the first stage of the study, metabolite production (exopolysaccharide, hydrogen peroxide and diacetyl production) and their bioactive properties (pH values, total acidity, total phenolic contents and antioxidant activities) were detected. In the second stage, poultry meat was inoculated with <i>Escherichia coli</i> O157:H7, <i>Listeria monocytogenes</i> and <i>Salmonella</i> Typhimurium (≈5 log CFU/g) using the spot inoculation method and the counts of pathogens were examined. In the final stage of the study, the counts of background microbiota (total mesophilic aerobic bacteria, total psychrophilic aerobic bacteria, <i>Pseudomonas</i> spp. Enterobacteriaceae and lactic acid bacteria) were determined.3. The amounts of exopolysaccharide, hydrogen peroxide and diacetyl were in the range of 156.00-514.30 µg/ml, 0.01-0.07 µg/ml and 19.15-75.54 ng/ml, respectively. The pH values, total acidity, total phenolic contents and antioxidant activities (DPPH and ABTS<sup>+</sup> radical scavenging activities) of the CFS were in the range of 4.08-4.34, 1.78-1.87 g lactic acid/100 ml, 3549-3864 mg GAE/l, 30.42-37.76% and 21.78-31.41%, respectively. In the second stage of the study, the counts of <i>E. coli</i> O157:H7, <i>L. monocytogenes</i> and <i>S</i>. Typhimurium inoculated on poultry meats were reduced by CFS treatment in the range of 0.82-1.41, 0.77-2.11 and 0.71-1.78 log CFU/g, respectively. In the final stage of the study, the counts of background microbiota on CFS-treated poultry meat samples were efficiently decreased during storage period (4°C for 7 d).4. The current study revealed that CFS are highly effective for ensuring meat safety and improving quality.</p>","PeriodicalId":9322,"journal":{"name":"British Poultry Science","volume":" ","pages":"1-13"},"PeriodicalIF":1.7000,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of cell-free supernatants produced by lactic acid bacteria on the safety and quality of poultry meat.\",\"authors\":\"B Ozturk, I Y Sengun\",\"doi\":\"10.1080/00071668.2025.2500344\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>1. This research characterised cell-free supernatants (CFS) obtained from lactic acid bacteria (<i>Leuconostoc pseudomesenteroides</i> T139, <i>Pediococcus acidilactici</i> T115 and <i>Streptococcus thermophilus</i> T127) and examined the effects of CFS (10%) on the safety and quality of poultry meat stored at 4°C for 7 d.2. In the first stage of the study, metabolite production (exopolysaccharide, hydrogen peroxide and diacetyl production) and their bioactive properties (pH values, total acidity, total phenolic contents and antioxidant activities) were detected. In the second stage, poultry meat was inoculated with <i>Escherichia coli</i> O157:H7, <i>Listeria monocytogenes</i> and <i>Salmonella</i> Typhimurium (≈5 log CFU/g) using the spot inoculation method and the counts of pathogens were examined. In the final stage of the study, the counts of background microbiota (total mesophilic aerobic bacteria, total psychrophilic aerobic bacteria, <i>Pseudomonas</i> spp. Enterobacteriaceae and lactic acid bacteria) were determined.3. The amounts of exopolysaccharide, hydrogen peroxide and diacetyl were in the range of 156.00-514.30 µg/ml, 0.01-0.07 µg/ml and 19.15-75.54 ng/ml, respectively. The pH values, total acidity, total phenolic contents and antioxidant activities (DPPH and ABTS<sup>+</sup> radical scavenging activities) of the CFS were in the range of 4.08-4.34, 1.78-1.87 g lactic acid/100 ml, 3549-3864 mg GAE/l, 30.42-37.76% and 21.78-31.41%, respectively. In the second stage of the study, the counts of <i>E. coli</i> O157:H7, <i>L. monocytogenes</i> and <i>S</i>. Typhimurium inoculated on poultry meats were reduced by CFS treatment in the range of 0.82-1.41, 0.77-2.11 and 0.71-1.78 log CFU/g, respectively. In the final stage of the study, the counts of background microbiota on CFS-treated poultry meat samples were efficiently decreased during storage period (4°C for 7 d).4. The current study revealed that CFS are highly effective for ensuring meat safety and improving quality.</p>\",\"PeriodicalId\":9322,\"journal\":{\"name\":\"British Poultry Science\",\"volume\":\" \",\"pages\":\"1-13\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-06-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"British Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/00071668.2025.2500344\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"British Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/00071668.2025.2500344","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0

摘要

1. 本研究对乳酸菌(假肠杆菌Leuconostoc pseudomesenteroides T139、嗜酸Pediococcus acid actitici T115和嗜热链球菌T127)的无细胞上清液(CFS)进行了表征,并检测了CFS(10%)对4°C保存7 d禽肉安全和质量的影响2。在研究的第一阶段,检测了代谢产物(胞外多糖、过氧化氢和双乙酰)的产生及其生物活性(pH值、总酸度、总酚含量和抗氧化活性)。第二阶段采用斑点接种法分别接种大肠杆菌O157:H7、单核增生李斯特菌和鼠伤寒沙门菌(≈5 log CFU/g),检测致病菌计数。在研究的最后阶段,测定了背景菌群的数量(嗜中温需氧菌总数、嗜冷需氧菌总数、假单胞菌、肠杆菌科和乳酸菌总数)。胞外多糖、双氧水和双乙酰含量分别为156.00 ~ 514.30µg/ml、0.01 ~ 0.07µg/ml和19.15 ~ 75.54 ng/ml。CFS的pH值、总酸度、总酚含量和抗氧化能力(DPPH和ABTS+自由基清除能力)分别为4.08 ~ 4.34、1.78 ~ 1.87 g乳酸/100 ml、3549 ~ 3864 mg GAE/l、30.42 ~ 37.76%和21.78 ~ 31.41%。在第二阶段的研究中,经CFS处理后,接种在家禽肉上的大肠杆菌O157:H7、单核增生乳杆菌L.和鼠伤寒沙门氏菌计数分别降低了0.82-1.41、0.77-2.11和0.71-1.78 log CFU/g。在研究的最后阶段,经过cfs处理的禽肉样品在4°C保存7 d期间的本底菌群计数有效降低。目前的研究表明,CFS对确保肉类安全和提高质量非常有效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of cell-free supernatants produced by lactic acid bacteria on the safety and quality of poultry meat.

1. This research characterised cell-free supernatants (CFS) obtained from lactic acid bacteria (Leuconostoc pseudomesenteroides T139, Pediococcus acidilactici T115 and Streptococcus thermophilus T127) and examined the effects of CFS (10%) on the safety and quality of poultry meat stored at 4°C for 7 d.2. In the first stage of the study, metabolite production (exopolysaccharide, hydrogen peroxide and diacetyl production) and their bioactive properties (pH values, total acidity, total phenolic contents and antioxidant activities) were detected. In the second stage, poultry meat was inoculated with Escherichia coli O157:H7, Listeria monocytogenes and Salmonella Typhimurium (≈5 log CFU/g) using the spot inoculation method and the counts of pathogens were examined. In the final stage of the study, the counts of background microbiota (total mesophilic aerobic bacteria, total psychrophilic aerobic bacteria, Pseudomonas spp. Enterobacteriaceae and lactic acid bacteria) were determined.3. The amounts of exopolysaccharide, hydrogen peroxide and diacetyl were in the range of 156.00-514.30 µg/ml, 0.01-0.07 µg/ml and 19.15-75.54 ng/ml, respectively. The pH values, total acidity, total phenolic contents and antioxidant activities (DPPH and ABTS+ radical scavenging activities) of the CFS were in the range of 4.08-4.34, 1.78-1.87 g lactic acid/100 ml, 3549-3864 mg GAE/l, 30.42-37.76% and 21.78-31.41%, respectively. In the second stage of the study, the counts of E. coli O157:H7, L. monocytogenes and S. Typhimurium inoculated on poultry meats were reduced by CFS treatment in the range of 0.82-1.41, 0.77-2.11 and 0.71-1.78 log CFU/g, respectively. In the final stage of the study, the counts of background microbiota on CFS-treated poultry meat samples were efficiently decreased during storage period (4°C for 7 d).4. The current study revealed that CFS are highly effective for ensuring meat safety and improving quality.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
British Poultry Science
British Poultry Science 农林科学-奶制品与动物科学
CiteScore
3.90
自引率
5.00%
发文量
88
审稿时长
4.5 months
期刊介绍: From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信