评估不同除垢方法对埃及伊蚊翅膀几何形态的影响。

IF 2.1 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2025-06-03 DOI:10.1016/j.actatropica.2025.107686

Fernanda Stefhany Alves Santos , Paulo Henrique Costa Corgosinho , Filipe Vieira Santos de Abreu , Marcela Sofia Vaca-Sánchez , Pablo Cuevas-Reyes , Maurício Lopes de Faria , Henrique Maia Valério , Magno Augusto Zazá Borges

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引用次数: 0

摘要

本研究旨在测试去除埃及伊蚊翅鳞的不同处理方法，并通过几何形态计量学来评估这些处理是否可以改变翼脉形态计量学模式。处理方法是使用微型超声波在矿泉水和氢氧化钠盐水溶液中搅拌机翼，使用零号(0)尖刷手动去除机翼鳞片。我们提出了一种使用甘油在载玻片上安装机翼的替代方法。我们得出的结论是，甘油用于幻灯片安装提供了更好的光学和半透明，而NaOH盐水溶液和水处理会损害翅膀的形态测量分析。尺寸为0(0)的尖端刷处理有效地去除了机翼鳞片，改善了机翼结构的可视化，而不会对样品造成重大修改。

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Assessing the impact of different scale removal methods on the geometric morphometrics of Aedes aegypti wings

This work aimed to test different treatments for removing wing scales from Aedes aegypti, and evaluate through geometric morphometrics, if these treatments can modify the wing venation morphometric pattern. The treatments were wing agitation in mineral water and NaOH (sodium hydroxide) saline solution using a mini-ultrasound, and manual wing scales removal using a size zero (0) tip brush. We propose an alternative method for mounting wings on slides using glycerin. We conclude that glycerin use for slide mounting provides better optics and translucency, and that treatments with NaOH saline solution and water can impair morphometric analysis of wings. The size zero (0) tip brush treatment efficiently removed wing scales, improving wing structure visualization without causing significant modifications to the specimens.

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.