探索非特异性过氧酶在非天然亚硝基化学中的作用。

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

ChemBioChem Pub Date : 2025-06-06 DOI:10.1002/cbic.202500332

Dominik Homann, Lewis Thomas-Hargreaves, Pascal Püllmann

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引用次数: 0

摘要

非特异性过氧酶（UPOs）具有较高的催化效率和反应稳定性。[1-5]在本报告中，我们将亚硝基化学添加到当前的UPO化学曲目中，利用廉价的商品化学羟胺作为共底物。1,2,3,4-四氢萘作为模型底物进行了研究，采用不同的UPOs面板，达到周转率高达745，对映体过剩值高达62%。总的来说，我们报告了关于非自然反应的UPO活动的第一个帐户。

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Probing Unspecific Peroxygenases for Non-Natural Nitrene Chemistry.

Unspecific peroxygenases (UPOs) perform challenging oxyfunctionalization chemistry with high catalytic efficiency and reaction stability. In this report, nitrene chemistry to the current repertoire of UPO chemistry is added, utilizing the inexpensive commodity chemical hydroxylamine as cosubstrate. 1,2,3,4-tetrahydronaphthalene is investigated as model substrate using a diverse panel of UPOs, reaching turnover numbers up to 745 and enantiomeric excess values up to 62%. Overall, the first account of UPO activity regarding non-natural nitrene activities is reported.

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来源期刊

ChemBioChem 生物-生化与分子生物学

CiteScore

6.10

自引率

3.10%

发文量

407

审稿时长

1 months

期刊介绍： ChemBioChem (Impact Factor 2018: 2.641) publishes important breakthroughs across all areas at the interface of chemistry and biology, including the fields of chemical biology, bioorganic chemistry, bioinorganic chemistry, synthetic biology, biocatalysis, bionanotechnology, and biomaterials. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies, and supported by the Asian Chemical Editorial Society (ACES).