Jelena Jadzic, Christine Plumeyer, Danica Djukic, Vladimir Zivkovic, Slobodan Nikolic, Annegreet Vlug, Nada Tomanovic, Marija Djuric, Petar Milovanovic, Björn Busse, Danijela Djonic
{"title":"酒精相关性肝病患者骨基质矿化、显微硬度和矿化骨细胞腔隙密度的位点特异性改变:对椎体骨折倾向的影响","authors":"Jelena Jadzic, Christine Plumeyer, Danica Djukic, Vladimir Zivkovic, Slobodan Nikolic, Annegreet Vlug, Nada Tomanovic, Marija Djuric, Petar Milovanovic, Björn Busse, Danijela Djonic","doi":"10.1093/jbmr/zjaf065","DOIUrl":null,"url":null,"abstract":"<p><p>Low bone quality has been reported as a source of bone fragility in individuals with alcohol-associated liver disease (AALD). Alterations of the bone matrix, including the spatial BMD distribution and osteocyte lacunar network characteristics, have not been comprehensively assessed in common fracture sites of individuals with AALD. Considering the clinical relevance of vertebral and femoral fragility fractures among these individuals, this cross-sectional study aimed to analyze site-specific BMD distribution, bone mechanical properties, osteocyte lacunar network, and Haversian system characteristics in the first lumbar vertebra and superolateral femoral neck of adult male donors with AALD. Quantitative backscattered electron imaging and Vickers micro-indentation testing were conducted to analyze bone quality in specimens from vertebral and femoral bone harvested at autopsy from adult male donors (n = 24), divided into 2 groups: AALD group (12 individuals with histopathological confirmation of AALD, age: 60 ± 12 yr) and age-matched controls (12 individuals without histopathological features of liver disease, age: 59 ± 12 yr). The comparative assessment revealed significantly lower mean calcium content, reduced Vickers micro-hardness, higher density of osteocyte lacunae occluded with mineralized matter (ie, micropetrosis), thicker osteonal wall, and a higher percentage of osteon refilling in lumbar vertebrae and superolateral femoral neck obtained from the AALD group (p < .05). Although bone quality alterations were congruent in both skeletal sites, the most severe AALD-induced impairment was noted in the trabecular compartment of lumbar vertebrae (p < .05). Our findings revealed a site-specific reduction in trabecular bone mineralization and an increased proportion of mineralized osteocyte lacunae in male individuals with AALD, which may contribute to increased vertebral predilection in these individuals.</p>","PeriodicalId":185,"journal":{"name":"Journal of Bone and Mineral Research","volume":" ","pages":"987-998"},"PeriodicalIF":5.9000,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Site-specific alterations of bone matrix mineralization, micro-hardness, and density of mineralized osteocyte lacunae in men with alcohol-associated liver disease: implications for vertebral fracture predilection.\",\"authors\":\"Jelena Jadzic, Christine Plumeyer, Danica Djukic, Vladimir Zivkovic, Slobodan Nikolic, Annegreet Vlug, Nada Tomanovic, Marija Djuric, Petar Milovanovic, Björn Busse, Danijela Djonic\",\"doi\":\"10.1093/jbmr/zjaf065\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Low bone quality has been reported as a source of bone fragility in individuals with alcohol-associated liver disease (AALD). Alterations of the bone matrix, including the spatial BMD distribution and osteocyte lacunar network characteristics, have not been comprehensively assessed in common fracture sites of individuals with AALD. Considering the clinical relevance of vertebral and femoral fragility fractures among these individuals, this cross-sectional study aimed to analyze site-specific BMD distribution, bone mechanical properties, osteocyte lacunar network, and Haversian system characteristics in the first lumbar vertebra and superolateral femoral neck of adult male donors with AALD. Quantitative backscattered electron imaging and Vickers micro-indentation testing were conducted to analyze bone quality in specimens from vertebral and femoral bone harvested at autopsy from adult male donors (n = 24), divided into 2 groups: AALD group (12 individuals with histopathological confirmation of AALD, age: 60 ± 12 yr) and age-matched controls (12 individuals without histopathological features of liver disease, age: 59 ± 12 yr). The comparative assessment revealed significantly lower mean calcium content, reduced Vickers micro-hardness, higher density of osteocyte lacunae occluded with mineralized matter (ie, micropetrosis), thicker osteonal wall, and a higher percentage of osteon refilling in lumbar vertebrae and superolateral femoral neck obtained from the AALD group (p < .05). Although bone quality alterations were congruent in both skeletal sites, the most severe AALD-induced impairment was noted in the trabecular compartment of lumbar vertebrae (p < .05). Our findings revealed a site-specific reduction in trabecular bone mineralization and an increased proportion of mineralized osteocyte lacunae in male individuals with AALD, which may contribute to increased vertebral predilection in these individuals.</p>\",\"PeriodicalId\":185,\"journal\":{\"name\":\"Journal of Bone and Mineral Research\",\"volume\":\" \",\"pages\":\"987-998\"},\"PeriodicalIF\":5.9000,\"publicationDate\":\"2025-07-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Bone and Mineral Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1093/jbmr/zjaf065\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Bone and Mineral Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/jbmr/zjaf065","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
Site-specific alterations of bone matrix mineralization, micro-hardness, and density of mineralized osteocyte lacunae in men with alcohol-associated liver disease: implications for vertebral fracture predilection.
Low bone quality has been reported as a source of bone fragility in individuals with alcohol-associated liver disease (AALD). Alterations of the bone matrix, including the spatial BMD distribution and osteocyte lacunar network characteristics, have not been comprehensively assessed in common fracture sites of individuals with AALD. Considering the clinical relevance of vertebral and femoral fragility fractures among these individuals, this cross-sectional study aimed to analyze site-specific BMD distribution, bone mechanical properties, osteocyte lacunar network, and Haversian system characteristics in the first lumbar vertebra and superolateral femoral neck of adult male donors with AALD. Quantitative backscattered electron imaging and Vickers micro-indentation testing were conducted to analyze bone quality in specimens from vertebral and femoral bone harvested at autopsy from adult male donors (n = 24), divided into 2 groups: AALD group (12 individuals with histopathological confirmation of AALD, age: 60 ± 12 yr) and age-matched controls (12 individuals without histopathological features of liver disease, age: 59 ± 12 yr). The comparative assessment revealed significantly lower mean calcium content, reduced Vickers micro-hardness, higher density of osteocyte lacunae occluded with mineralized matter (ie, micropetrosis), thicker osteonal wall, and a higher percentage of osteon refilling in lumbar vertebrae and superolateral femoral neck obtained from the AALD group (p < .05). Although bone quality alterations were congruent in both skeletal sites, the most severe AALD-induced impairment was noted in the trabecular compartment of lumbar vertebrae (p < .05). Our findings revealed a site-specific reduction in trabecular bone mineralization and an increased proportion of mineralized osteocyte lacunae in male individuals with AALD, which may contribute to increased vertebral predilection in these individuals.
期刊介绍:
The Journal of Bone and Mineral Research (JBMR) publishes highly impactful original manuscripts, reviews, and special articles on basic, translational and clinical investigations relevant to the musculoskeletal system and mineral metabolism. Specifically, the journal is interested in original research on the biology and physiology of skeletal tissues, interdisciplinary research spanning the musculoskeletal and other systems, including but not limited to immunology, hematology, energy metabolism, cancer biology, and neurology, and systems biology topics using large scale “-omics” approaches. The journal welcomes clinical research on the pathophysiology, treatment and prevention of osteoporosis and fractures, as well as sarcopenia, disorders of bone and mineral metabolism, and rare or genetically determined bone diseases.