{"title":"基于血浆蛋白质组学的糖尿病足新的预测和诊断炎症生物标志物鉴定。","authors":"Yuchao Feng, Binyuan Jiang, Qianzhi Luo, Chenchen Liao, Mingjiang Liu, Liangdong Jiang","doi":"10.1021/acs.jproteome.5c00037","DOIUrl":null,"url":null,"abstract":"<p><p>Diabetic foot ulceration (DFU) is a severe complication of diabetes characterized by chronic inflammation, yet its underlying mechanisms remain unclear. This study aimed to identify plasma inflammatory biomarkers distinguishing diabetic foot (DF) patients from those with diabetes (DM) and healthy controls (NC). Using proximity extension assay (PEA) technology, we analyzed 92 inflammation-related proteins in fasting plasma samples from NC (<i>n</i> = 10), DM (<i>n</i> = 10), and DF (<i>n</i> = 10) groups. Compared to DM patients, DF patients exhibited significant upregulation of 21 cytokines. Among these, MCP-4, SLAMF1, CD5, CSF-1, and FGF-5 demonstrated high diagnostic potential with AUC values ranging from 0.84 to 0.92. ELISA validation confirmed the elevated expression of MCP-4, SLAMF1, and CSF-1 in DF patients. Functional enrichment analysis linked these proteins to inflammatory pathways (e.g., cytokine-cytokine receptor interaction) and macrophage activation. This study highlights MCP-4, SLAMF1, and CSF-1 as novel predictive biomarkers for DF, providing insights into its pathogenesis and diagnostic strategies.</p>","PeriodicalId":48,"journal":{"name":"Journal of Proteome Research","volume":"24 6","pages":"3017-3031"},"PeriodicalIF":3.6000,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Plasma Proteomics-Based Identification of Novel Predictive and Diagnostic Inflammatory Biomarkers for Diabetic Foot.\",\"authors\":\"Yuchao Feng, Binyuan Jiang, Qianzhi Luo, Chenchen Liao, Mingjiang Liu, Liangdong Jiang\",\"doi\":\"10.1021/acs.jproteome.5c00037\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Diabetic foot ulceration (DFU) is a severe complication of diabetes characterized by chronic inflammation, yet its underlying mechanisms remain unclear. This study aimed to identify plasma inflammatory biomarkers distinguishing diabetic foot (DF) patients from those with diabetes (DM) and healthy controls (NC). Using proximity extension assay (PEA) technology, we analyzed 92 inflammation-related proteins in fasting plasma samples from NC (<i>n</i> = 10), DM (<i>n</i> = 10), and DF (<i>n</i> = 10) groups. Compared to DM patients, DF patients exhibited significant upregulation of 21 cytokines. Among these, MCP-4, SLAMF1, CD5, CSF-1, and FGF-5 demonstrated high diagnostic potential with AUC values ranging from 0.84 to 0.92. ELISA validation confirmed the elevated expression of MCP-4, SLAMF1, and CSF-1 in DF patients. Functional enrichment analysis linked these proteins to inflammatory pathways (e.g., cytokine-cytokine receptor interaction) and macrophage activation. This study highlights MCP-4, SLAMF1, and CSF-1 as novel predictive biomarkers for DF, providing insights into its pathogenesis and diagnostic strategies.</p>\",\"PeriodicalId\":48,\"journal\":{\"name\":\"Journal of Proteome Research\",\"volume\":\"24 6\",\"pages\":\"3017-3031\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2025-06-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Proteome Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.jproteome.5c00037\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Proteome Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1021/acs.jproteome.5c00037","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/7 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Plasma Proteomics-Based Identification of Novel Predictive and Diagnostic Inflammatory Biomarkers for Diabetic Foot.
Diabetic foot ulceration (DFU) is a severe complication of diabetes characterized by chronic inflammation, yet its underlying mechanisms remain unclear. This study aimed to identify plasma inflammatory biomarkers distinguishing diabetic foot (DF) patients from those with diabetes (DM) and healthy controls (NC). Using proximity extension assay (PEA) technology, we analyzed 92 inflammation-related proteins in fasting plasma samples from NC (n = 10), DM (n = 10), and DF (n = 10) groups. Compared to DM patients, DF patients exhibited significant upregulation of 21 cytokines. Among these, MCP-4, SLAMF1, CD5, CSF-1, and FGF-5 demonstrated high diagnostic potential with AUC values ranging from 0.84 to 0.92. ELISA validation confirmed the elevated expression of MCP-4, SLAMF1, and CSF-1 in DF patients. Functional enrichment analysis linked these proteins to inflammatory pathways (e.g., cytokine-cytokine receptor interaction) and macrophage activation. This study highlights MCP-4, SLAMF1, and CSF-1 as novel predictive biomarkers for DF, providing insights into its pathogenesis and diagnostic strategies.
期刊介绍：
Journal of Proteome Research publishes content encompassing all aspects of global protein analysis and function, including the dynamic aspects of genomics, spatio-temporal proteomics, metabonomics and metabolomics, clinical and agricultural proteomics, as well as advances in methodology including bioinformatics. The theme and emphasis is on a multidisciplinary approach to the life sciences through the synergy between the different types of "omics".
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