Xiuwen Guo , Liquan Dong , Xin Wang , Rujun Dai , Jinyu Li , Shujun Liu , Lingqin Kong , Ming Liu , Yuejin Zhao , Mei Hui , Xuhong Chu
{"title":"PLAM系统介导的808 nm低功率激光密度对人脐带间充质干细胞生物活性增强的优化","authors":"Xiuwen Guo , Liquan Dong , Xin Wang , Rujun Dai , Jinyu Li , Shujun Liu , Lingqin Kong , Ming Liu , Yuejin Zhao , Mei Hui , Xuhong Chu","doi":"10.1016/j.optlaseng.2025.109129","DOIUrl":null,"url":null,"abstract":"<div><div>Low-level laser therapy (LLLT) has significant potential in regenerative medicine for enhancing mesenchymal stem cells (MSCs) function. However, the optimal irradiation parameters, particularly power density, remain unclear. In this study, we developed an automated Precision Laser Adjustment and Motion (PLAM) system to investigate the effects of an 808 nm power-adjustable laser on human umbilical cord mesenchymal stem cells (HUMSCs). The laser was applied at power densities ranging from 10 to 500 mW/cm², with a constant energy density of 4 J/cm². Under these irradiation conditions, the biological responses showed clear power-dependence, with optimal effects observed at 100 and 250 mW/cm², consistent with the Arndt-Schulz law. Specifically, the 250 mW/cm² irradiation significantly accelerated cell migration at 24 hours and achieved the highest cell viability at 48 hours (<em>P</em> < 0.01). Notably, the 100 mW/cm² protocol demonstrated superior biosafety, maintaining sustained ATP production (<em>p</em> < 0.001 at 24 hours; <em>p</em> < 0.01 at 48 hours) and moderate reactive oxygen species (ROS) levels (<em>p</em> < 0.0001), making it reliable for long-term stem cells expansion. Real-time temperature monitoring showed ΔT ≤1 °C, ruling out thermal damage. The results emphasize the importance of optimizing power density in LLLT and identify 100 mW/cm² as the optimal balance between therapeutic efficacy and safety. Furthermore, the PLAM system simplifies the irradiation procedure, reduces operational errors, and enhances experimental repeatability. These findings provide a scientific basis for standardizing LLLT parameters in regenerative medicine and introduce the PLAM automated experimental platform for future photobiomodulation research.</div></div>","PeriodicalId":49719,"journal":{"name":"Optics and Lasers in Engineering","volume":"194 ","pages":"Article 109129"},"PeriodicalIF":3.5000,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"PLAM system-mediated optimization of 808 nm low-level laser power density for enhanced bioactivity in human umbilical cord mesenchymal stem cells\",\"authors\":\"Xiuwen Guo , Liquan Dong , Xin Wang , Rujun Dai , Jinyu Li , Shujun Liu , Lingqin Kong , Ming Liu , Yuejin Zhao , Mei Hui , Xuhong Chu\",\"doi\":\"10.1016/j.optlaseng.2025.109129\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Low-level laser therapy (LLLT) has significant potential in regenerative medicine for enhancing mesenchymal stem cells (MSCs) function. However, the optimal irradiation parameters, particularly power density, remain unclear. In this study, we developed an automated Precision Laser Adjustment and Motion (PLAM) system to investigate the effects of an 808 nm power-adjustable laser on human umbilical cord mesenchymal stem cells (HUMSCs). The laser was applied at power densities ranging from 10 to 500 mW/cm², with a constant energy density of 4 J/cm². Under these irradiation conditions, the biological responses showed clear power-dependence, with optimal effects observed at 100 and 250 mW/cm², consistent with the Arndt-Schulz law. Specifically, the 250 mW/cm² irradiation significantly accelerated cell migration at 24 hours and achieved the highest cell viability at 48 hours (<em>P</em> < 0.01). Notably, the 100 mW/cm² protocol demonstrated superior biosafety, maintaining sustained ATP production (<em>p</em> < 0.001 at 24 hours; <em>p</em> < 0.01 at 48 hours) and moderate reactive oxygen species (ROS) levels (<em>p</em> < 0.0001), making it reliable for long-term stem cells expansion. Real-time temperature monitoring showed ΔT ≤1 °C, ruling out thermal damage. The results emphasize the importance of optimizing power density in LLLT and identify 100 mW/cm² as the optimal balance between therapeutic efficacy and safety. Furthermore, the PLAM system simplifies the irradiation procedure, reduces operational errors, and enhances experimental repeatability. These findings provide a scientific basis for standardizing LLLT parameters in regenerative medicine and introduce the PLAM automated experimental platform for future photobiomodulation research.</div></div>\",\"PeriodicalId\":49719,\"journal\":{\"name\":\"Optics and Lasers in Engineering\",\"volume\":\"194 \",\"pages\":\"Article 109129\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-06-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Optics and Lasers in Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0143816625003148\",\"RegionNum\":2,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"OPTICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optics and Lasers in Engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0143816625003148","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPTICS","Score":null,"Total":0}
PLAM system-mediated optimization of 808 nm low-level laser power density for enhanced bioactivity in human umbilical cord mesenchymal stem cells
Low-level laser therapy (LLLT) has significant potential in regenerative medicine for enhancing mesenchymal stem cells (MSCs) function. However, the optimal irradiation parameters, particularly power density, remain unclear. In this study, we developed an automated Precision Laser Adjustment and Motion (PLAM) system to investigate the effects of an 808 nm power-adjustable laser on human umbilical cord mesenchymal stem cells (HUMSCs). The laser was applied at power densities ranging from 10 to 500 mW/cm², with a constant energy density of 4 J/cm². Under these irradiation conditions, the biological responses showed clear power-dependence, with optimal effects observed at 100 and 250 mW/cm², consistent with the Arndt-Schulz law. Specifically, the 250 mW/cm² irradiation significantly accelerated cell migration at 24 hours and achieved the highest cell viability at 48 hours (P < 0.01). Notably, the 100 mW/cm² protocol demonstrated superior biosafety, maintaining sustained ATP production (p < 0.001 at 24 hours; p < 0.01 at 48 hours) and moderate reactive oxygen species (ROS) levels (p < 0.0001), making it reliable for long-term stem cells expansion. Real-time temperature monitoring showed ΔT ≤1 °C, ruling out thermal damage. The results emphasize the importance of optimizing power density in LLLT and identify 100 mW/cm² as the optimal balance between therapeutic efficacy and safety. Furthermore, the PLAM system simplifies the irradiation procedure, reduces operational errors, and enhances experimental repeatability. These findings provide a scientific basis for standardizing LLLT parameters in regenerative medicine and introduce the PLAM automated experimental platform for future photobiomodulation research.
期刊介绍:
Optics and Lasers in Engineering aims at providing an international forum for the interchange of information on the development of optical techniques and laser technology in engineering. Emphasis is placed on contributions targeted at the practical use of methods and devices, the development and enhancement of solutions and new theoretical concepts for experimental methods.
Optics and Lasers in Engineering reflects the main areas in which optical methods are being used and developed for an engineering environment. Manuscripts should offer clear evidence of novelty and significance. Papers focusing on parameter optimization or computational issues are not suitable. Similarly, papers focussed on an application rather than the optical method fall outside the journal''s scope. The scope of the journal is defined to include the following:
-Optical Metrology-
Optical Methods for 3D visualization and virtual engineering-
Optical Techniques for Microsystems-
Imaging, Microscopy and Adaptive Optics-
Computational Imaging-
Laser methods in manufacturing-
Integrated optical and photonic sensors-
Optics and Photonics in Life Science-
Hyperspectral and spectroscopic methods-
Infrared and Terahertz techniques