二尖瓣主动脉瓣男婴诱导多能干细胞系（NCHi024-A）的鉴定

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-06-03 DOI:10.1016/j.scr.2025.103751

Jiyoon Lee , Meghan Hanley , Shiqiao Ye , Yang Yu , Aaron Argall , Ming-Tao Zhao

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引用次数: 0

摘要

二尖瓣主动脉瓣是最常见的先天性心脏缺陷之一，它只有两个小叶而不是三个。NCHi024-A是一种诱导多能干细胞（iPSC）系，来源于患有二尖瓣主动脉瓣的男婴外周血单个核细胞（PBMCs）。该细胞系显示正常的iPSC形态、正常的核型和供体匹配的身份。通过免疫荧光染色验证未分化iPSC和分化为外胚层、中胚层和内胚层的标记。NCHi024-A转基因和支原体污染检测均为阴性。该iPSC细胞系可分化为多种类型的细胞，用于主动脉瓣疾病的研究。

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Characterization of an induced pluripotent stem cell line (NCHi024-A) from a male infant with bicuspid aortic valve

Bicuspid aortic valve is one of the most common congenital heart defects which have only two leaflets instead of three. NCHi024-A is an induced pluripotent stem cell (iPSC) line derived from peripheral blood mononuclear cells (PBMCs) of a male infant with a bicuspid aortic valve. The line exhibits normal iPSC morphology, normal karyotype, and donor-matched identity. Markers of undifferentiated iPSC and differentiation into ectoderm, mesoderm, and endoderm were validated through immunofluorescence staining. NCHi024-A was tested negative for transgenes and mycoplasma contamination. This iPSC line can be differentiated into various types of cells and used for studying aortic valve disease.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.