A J Silva, T J Dias, J H B Silva, A C Bezerra, V F O Sousa, A H P C Martins, F S Bezerra, T I Silva, A V Silva, R N M Costa
{"title":"受氮钾比和生物刺激素剂量影响的甜椒叶绿素荧光","authors":"A J Silva, T J Dias, J H B Silva, A C Bezerra, V F O Sousa, A H P C Martins, F S Bezerra, T I Silva, A V Silva, R N M Costa","doi":"10.1590/1519-6984.295013","DOIUrl":null,"url":null,"abstract":"<p><p>Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments.</p>","PeriodicalId":55326,"journal":{"name":"Brazilian Journal of Biology","volume":"85 ","pages":"e295013"},"PeriodicalIF":0.0000,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chlorophyll fluorescence in sweet pepper subjected to N:K ratios and biostimulant doses.\",\"authors\":\"A J Silva, T J Dias, J H B Silva, A C Bezerra, V F O Sousa, A H P C Martins, F S Bezerra, T I Silva, A V Silva, R N M Costa\",\"doi\":\"10.1590/1519-6984.295013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments.</p>\",\"PeriodicalId\":55326,\"journal\":{\"name\":\"Brazilian Journal of Biology\",\"volume\":\"85 \",\"pages\":\"e295013\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-06-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brazilian Journal of Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1590/1519-6984.295013\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/1519-6984.295013","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
摘要
甜椒在巴西是一种具有重要经济意义的蔬菜。这种蔬菜在全国广泛种植和消费,估计种植面积为1.9万公顷,产量超过42万吨。因此,叶片叶绿素含量分析是农业上常用的一种方法,具有监测植物发育的功能,为植物的生理状态、叶片含氮量和光合潜能提供重要信息。本研究对不同氮钾比和不同剂量生物刺激素处理下的甜椒植株进行了叶绿素荧光a、b和叶绿素总含量的测定。试验于2020年3月至7月在Areia - PB市农村地区Engenho Triunfo进行,采用随机区组设计,按5 × 5因子方案,在细分样地布置,主样地设置5个N:K比(1:1,1:2,2:1,1:3;3:1)和5个剂量的生物刺激素(0;1.12;2.25;3.37和4.5 mL L-1)。分析的变量为叶绿素荧光:初始荧光、最大荧光、可变荧光。分析了叶绿素指数:叶绿素a、叶绿素b和叶绿素总含量。方差分析显示,各因子、生物刺激素剂量和N:K比的交互作用对辣椒种植后30和60 d的光化学活性和光合色素无显著影响,两种因素单独作用对辣椒的光化学活性和光合色素无显著影响。从这个意义上讲,采用置信区间(波段)来证明每个N:K比率作为所研究变量的生物刺激素浓度增加的函数的平均效应。当N:K比为1:2和2:1时,不需要使用生物刺激剂来增强叶绿素荧光和光合色素的合成。
Chlorophyll fluorescence in sweet pepper subjected to N:K ratios and biostimulant doses.
Sweet pepper are a vegetable of great economic importance in Brazil. This vegetable is widely cultivated and consumed throughout the country, with an estimated planting area of 19 thousand hectares and production of over 420 thousand tons. Analysis of chlorophyll content in leaves is therefore a method commonly adopted in agriculture, with the function of monitoring plant development, providing important information on the physiological state, nitrogen content in leaves and the photosynthetic potential of plants. In this research, chlorophyll fluorescence was evaluated a, b and total chlorophyll was evaluated in bell pepper plants subjected to N:K ratio and doses of biostimulant. The experiment was carried out from March to July 2020, at Engenho Triunfo, rural area of the municipality of Areia - PB, using a randomized block design, arranged in subdivided plots, in a 5 × 5 factorial scheme, with five N:K ratios in the main plot (1:1, 1:2, 2:1, 1:3; 3:1) and five doses of biostimulant in the subplot (0; 1.12; 2.25; 3.37 and 4.5 mL L-1). The variables analyzed were chlorophyll fluorescence: initial, maximum, variable fluorescence. The chlorophyll indices were also analyzed: chlorophylls a, b and total. The analysis of variance showed no significant effect in the interaction of factors, biostimulant doses and N:K ratios, and no significant effect for both factors alone on the photochemical activity and photosynthetic pigments of pepper at 30 and 60 days after planting (DAP). In this sense, confidence intervals (bands) were performed to demonstrate the average effect of each N:K ratio as a function of the increase in the biostimulant concentration for the variables studied. The use of N:K ratios 1:2 and 2:1 did not require the use of the biostimulant for greater chlorophyll fluorescence and synthesis of photosynthetic pigments.
期刊介绍:
The BJB – Brazilian Journal of Biology® is a scientific journal devoted to publishing original articles in all fields of the Biological Sciences, i.e., General Biology, Cell Biology, Evolution, Biological Oceanography, Taxonomy, Geographic Distribution, Limnology, Aquatic Biology, Botany, Zoology, Genetics, and Ecology. Priority is given to papers presenting results of researches in the Neotropical region. Material published includes research papers, review papers (upon approval of the Editorial Board), notes, book reviews, and comments.