新鲜和福尔马林固定脑的基本超声特性参数图像可视化。

IF 2.6 3区医学 Q2 ACOUSTICS

Ultrasound in Medicine and Biology Pub Date : 2025-06-03 DOI:10.1016/j.ultrasmedbio.2025.04.020

Brent K. Hoffmeister , Grant R. Jenson , Amalia M. Bay , Ann M. Viano , Phyu Sin M. Myat , Cecille Labuda , Grace I. Nehring , Emily E. Bingham , Blake C. Lawler , Kate E. Hazelwood

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引用次数: 0

摘要

目的：脑是一个复杂的器官，具有多种组织类型和复杂的叶、褶、脑室等结构形态。本研究的目的是建立四种超声参数：声速（SOS）、衰减频率斜率（FSA）、综合后向散射系数（IBC）和视综合后向散射（AIB）在福尔马林固定前后脑组织的详细参数图像。方法：从9只牛的矢状面和冠状面制备23块1 cm厚的脑组织切片。超声波测量使用浸入式扫描系统进行，该系统配备了以615 μm步长移动的5 MHz聚焦换能器。结果：所有新鲜组织标本的测量值，以平均值±标准差（代表标本平均值之间的差异）为平均值，SOS的测量值为（1535±2）m/s， FSA的测量值为（0.546±0.037）dB/cm/MHz， IBC的测量值为（0.402±0.165）× 10-3 cm-1 str-1， AIB的测量值为（-60.1±1.1）dB相对于平面玻璃反射器。脑白质SOS、FSA值较高，AIB、IBC值较低。福尔马林固定导致SOS增加0.6%，AIB增加2%，FSA增加20%，IBC平均增加55%。结论：在大多数参数化图像中，组织结构和白质清晰可见。福尔马林固定在所有四个超声参数产生显著变化。

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Fundamental Ultrasonic Properties of Fresh and Formalin Fixed Brain Visualized as Parametric Images

Objective

The brain is a complex organ with multiple tissue types and a complicated morphology of lobes, folds, ventricles and other structures. The goal of this study was to create detailed parametric images of brain tissue before and after formalin fixation for four ultrasonic parameters: speed of sound (SOS), frequency slope of attenuation (FSA), integrated backscatter coefficient (IBC) and apparent integrated backscatter (AIB).

Methods

Twenty-three, 1-cm thick slices of brain tissue were prepared from the sagittal and coronal planes of nine bovine brains. Ultrasonic measurements were performed using an immersion scanning system equipped with a 5 MHz focused transducer moved in 615 μm steps.

Results

Measured values, reported as mean ± standard deviation (representing variation between specimen means) averaged over all measurements on all specimens of fresh tissue, were (1535 ± 2) m/s for SOS, (0.546 ± 0.037) dB/cm/MHz for FSA, (0.402 ± 0.165) × 10⁻³ cm⁻¹ str⁻¹ for IBC and (-60.1 ± 1.1) dB for AIB measured relative to a planar glass reflector. Regions of white matter were characterized by higher values of SOS and FSA, and lower values of AIB and IBC. Formalin fixation caused up to a 0.6% increase in SOS, up to a 2% increase in AIB, up to a 20% increase in FSA and up to a 55% increase in IBC averaged over all measurements on all specimens.

Conclusion

Tissue structures and white matter were clearly distinguishable in most parametric images. Formalin fixation produced significant changes in all four ultrasonic parameters.

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来源期刊

Ultrasound in Medicine and Biology 医学-核医学

CiteScore

6.20

自引率

6.90%

发文量

325

审稿时长

70 days

期刊介绍： Ultrasound in Medicine and Biology is the official journal of the World Federation for Ultrasound in Medicine and Biology. The journal publishes original contributions that demonstrate a novel application of an existing ultrasound technology in clinical diagnostic, interventional and therapeutic applications, new and improved clinical techniques, the physics, engineering and technology of ultrasound in medicine and biology, and the interactions between ultrasound and biological systems, including bioeffects. Papers that simply utilize standard diagnostic ultrasound as a measuring tool will be considered out of scope. Extended critical reviews of subjects of contemporary interest in the field are also published, in addition to occasional editorial articles, clinical and technical notes, book reviews, letters to the editor and a calendar of forthcoming meetings. It is the aim of the journal fully to meet the information and publication requirements of the clinicians, scientists, engineers and other professionals who constitute the biomedical ultrasonic community.