超声引导下坐骨神经亚甲基蓝染色与蓝色组织标记物染色的比较。

IF 1.9 2区农林科学 Q2 VETERINARY SCIENCES

Veterinary anaesthesia and analgesia Pub Date : 2025-05-08 DOI:10.1016/j.vaa.2025.05.002

Douglas Castro, Latchmi H.K. Baba, Susan M. Elrod, Jacob A. Johnson, Stuart C. Clark-Price, Erik H. Hofmeister

{"title":"超声引导下坐骨神经亚甲基蓝染色与蓝色组织标记物染色的比较。","authors":"Douglas Castro, Latchmi H.K. Baba, Susan M. Elrod, Jacob A. Johnson, Stuart C. Clark-Price, Erik H. Hofmeister","doi":"10.1016/j.vaa.2025.05.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>To compare the use of methylene blue and blue tissue marker in achieving sciatic nerve staining in cadaveric rats after perineural or intramuscular injection.</div></div><div><h3>Study design</h3><div>Experimental, randomized, blinded, crossover cadaveric study.</div></div><div><h3>Animals</h3><div>A group of 16 fresh-frozen adult Wistar rat cadavers.</div></div><div><h3>Methods</h3><div>Phase I: ultrasound-guided sciatic nerve injections were performed using either methylene blue (Group Methb, <em>n</em> = 8) or a blue tissue marker (Group Tmarker, <em>n</em> = 8). Phase II: ultrasound-guided <em>biceps femoris</em> intramuscular injections were performed with the same dyes (Group Methb IM, <em>n</em> = 8; Group Tmarker IM, <em>n</em> = 8). Volume of each injection was 0.1 mL, followed by a 5 minute interval before anatomical dissection. Positive staining was measured along the sciatic nerve in millimeters. Data analysis included <em>t</em> tests for parametric data and Wilcoxon and Fisher’s exact tests for nonparametric data, with significance set at <em>p</em> < 0.05.</div></div><div><h3>Results</h3><div>Phase I: both solutions consistently stained the sciatic nerve in all pelvic limbs. However, the length of staining was significantly greater in Group Methb (18 ± 1.9 mm) than in Group Tmarker (4.7 ± 1.3 mm) (<em>p</em> < 0.001). Phase II: sciatic nerve staining was observed in the Group Methb IM (7/7, 100%), with a median spread of 12 mm (interquartile range 11–12 mm), whereas no staining was detected in the Group Tmarker IM (0/8) (<em>p</em> = 0.015).</div></div><div><h3>Conclusions</h3><div>and clinical relevance Methylene blue achieved greater staining along the sciatic nerve than blue tissue marker. Furthermore, methylene blue diffused through the <em>biceps femoris</em>, effectively staining the sciatic nerve and surrounding tissues. This difference highlights the potential for overestimation in studies that use methylene blue and underscores the importance of selecting appropriate dye solutions.</div></div>","PeriodicalId":23626,"journal":{"name":"Veterinary anaesthesia and analgesia","volume":"52 4","pages":"Pages 480-487"},"PeriodicalIF":1.9000,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of ultrasound-guided sciatic nerve staining with methylene blue or blue tissue marker\",\"authors\":\"Douglas Castro, Latchmi H.K. Baba, Susan M. Elrod, Jacob A. Johnson, Stuart C. Clark-Price, Erik H. Hofmeister\",\"doi\":\"10.1016/j.vaa.2025.05.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div>To compare the use of methylene blue and blue tissue marker in achieving sciatic nerve staining in cadaveric rats after perineural or intramuscular injection.</div></div><div><h3>Study design</h3><div>Experimental, randomized, blinded, crossover cadaveric study.</div></div><div><h3>Animals</h3><div>A group of 16 fresh-frozen adult Wistar rat cadavers.</div></div><div><h3>Methods</h3><div>Phase I: ultrasound-guided sciatic nerve injections were performed using either methylene blue (Group Methb, <em>n</em> = 8) or a blue tissue marker (Group Tmarker, <em>n</em> = 8). Phase II: ultrasound-guided <em>biceps femoris</em> intramuscular injections were performed with the same dyes (Group Methb IM, <em>n</em> = 8; Group Tmarker IM, <em>n</em> = 8). Volume of each injection was 0.1 mL, followed by a 5 minute interval before anatomical dissection. Positive staining was measured along the sciatic nerve in millimeters. Data analysis included <em>t</em> tests for parametric data and Wilcoxon and Fisher’s exact tests for nonparametric data, with significance set at <em>p</em> < 0.05.</div></div><div><h3>Results</h3><div>Phase I: both solutions consistently stained the sciatic nerve in all pelvic limbs. However, the length of staining was significantly greater in Group Methb (18 ± 1.9 mm) than in Group Tmarker (4.7 ± 1.3 mm) (<em>p</em> < 0.001). Phase II: sciatic nerve staining was observed in the Group Methb IM (7/7, 100%), with a median spread of 12 mm (interquartile range 11–12 mm), whereas no staining was detected in the Group Tmarker IM (0/8) (<em>p</em> = 0.015).</div></div><div><h3>Conclusions</h3><div>and clinical relevance Methylene blue achieved greater staining along the sciatic nerve than blue tissue marker. Furthermore, methylene blue diffused through the <em>biceps femoris</em>, effectively staining the sciatic nerve and surrounding tissues. This difference highlights the potential for overestimation in studies that use methylene blue and underscores the importance of selecting appropriate dye solutions.</div></div>\",\"PeriodicalId\":23626,\"journal\":{\"name\":\"Veterinary anaesthesia and analgesia\",\"volume\":\"52 4\",\"pages\":\"Pages 480-487\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2025-05-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary anaesthesia and analgesia\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1467298725001072\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary anaesthesia and analgesia","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1467298725001072","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

目的：比较亚甲基蓝和蓝色组织标记物在大鼠尸体神经周围和肌肉注射后坐骨神经染色中的应用。研究设计：实验、随机、盲法、交叉尸体研究。动物：一组16具新鲜冷冻的成年Wistar老鼠尸体。方法：第一阶段：超声引导坐骨神经注射，使用亚甲基蓝（Methb组，n = 8）或蓝色组织标记物（Tmarker组，n = 8）。第二阶段：超声引导下用相同的染料进行股二头肌肌内注射(IM组，n = 8；组标记为IM， n = 8)。每次注射量0.1 mL，间隔5分钟解剖解剖。沿坐骨神经呈阳性染色，以毫米为单位。数据分析包括参数数据的t检验和非参数数据的Wilcoxon和Fisher精确检验，显著性设置为p < 0.05。结果：第一阶段：两种溶液一致染色所有骨盆肢体的坐骨神经。然而，Methb组染色长度（18±1.9 mm）明显大于Tmarker组（4.7±1.3 mm）（p < 0.001）。ii期：IM组（7/7,100%）观察到坐骨神经染色，中位分布为12 mm（四分位数范围11-12 mm），而IM组（0/8）未检测到染色（p = 0.015）。结论：与临床相关性相比，亚甲基蓝在坐骨神经上的染色效果更好。此外，亚甲基蓝通过股二头肌扩散，有效地染色了坐骨神经和周围组织。这种差异突出了在使用亚甲基蓝的研究中可能高估的可能性，并强调了选择合适的染料溶液的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Comparison of ultrasound-guided sciatic nerve staining with methylene blue or blue tissue marker

Objective

To compare the use of methylene blue and blue tissue marker in achieving sciatic nerve staining in cadaveric rats after perineural or intramuscular injection.

Study design

Experimental, randomized, blinded, crossover cadaveric study.

Animals

A group of 16 fresh-frozen adult Wistar rat cadavers.

Methods

Phase I: ultrasound-guided sciatic nerve injections were performed using either methylene blue (Group Methb, n = 8) or a blue tissue marker (Group Tmarker, n = 8). Phase II: ultrasound-guided biceps femoris intramuscular injections were performed with the same dyes (Group Methb IM, n = 8; Group Tmarker IM, n = 8). Volume of each injection was 0.1 mL, followed by a 5 minute interval before anatomical dissection. Positive staining was measured along the sciatic nerve in millimeters. Data analysis included t tests for parametric data and Wilcoxon and Fisher’s exact tests for nonparametric data, with significance set at p < 0.05.

Results

Phase I: both solutions consistently stained the sciatic nerve in all pelvic limbs. However, the length of staining was significantly greater in Group Methb (18 ± 1.9 mm) than in Group Tmarker (4.7 ± 1.3 mm) (p < 0.001). Phase II: sciatic nerve staining was observed in the Group Methb IM (7/7, 100%), with a median spread of 12 mm (interquartile range 11–12 mm), whereas no staining was detected in the Group Tmarker IM (0/8) (p = 0.015).

Conclusions

and clinical relevance Methylene blue achieved greater staining along the sciatic nerve than blue tissue marker. Furthermore, methylene blue diffused through the biceps femoris, effectively staining the sciatic nerve and surrounding tissues. This difference highlights the potential for overestimation in studies that use methylene blue and underscores the importance of selecting appropriate dye solutions.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Veterinary anaesthesia and analgesia 农林科学-兽医学

CiteScore

3.10

自引率

17.60%

发文量

审稿时长

97 days

期刊介绍： Veterinary Anaesthesia and Analgesia is the official journal of the Association of Veterinary Anaesthetists, the American College of Veterinary Anesthesia and Analgesia and the European College of Veterinary Anaesthesia and Analgesia. Its purpose is the publication of original, peer reviewed articles covering all branches of anaesthesia and the relief of pain in animals. Articles concerned with the following subjects related to anaesthesia and analgesia are also welcome: the basic sciences; pathophysiology of disease as it relates to anaesthetic management equipment intensive care chemical restraint of animals including laboratory animals, wildlife and exotic animals welfare issues associated with pain and distress education in veterinary anaesthesia and analgesia. Review articles, special articles, and historical notes will also be published, along with editorials, case reports in the form of letters to the editor, and book reviews. There is also an active correspondence section.