壳聚糖金纳米颗粒包埋诺比叶素的ph调控释放特性通过PI3K/AKT/mTOR信号通路诱导胃癌细胞凋亡。

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2025-05-31 DOI:10.1080/02652048.2025.2507638

Hongyi Qing, Ping Xie, Zhenjiang Wu, Wenhai Fan, Yuanxiao Liang, Xiulian Xu

{"title":"壳聚糖金纳米颗粒包埋诺比叶素的ph调控释放特性通过PI3K/AKT/mTOR信号通路诱导胃癌细胞凋亡。","authors":"Hongyi Qing, Ping Xie, Zhenjiang Wu, Wenhai Fan, Yuanxiao Liang, Xiulian Xu","doi":"10.1080/02652048.2025.2507638","DOIUrl":null,"url":null,"abstract":"Context: Gastric cancer (GC) remains a major health concern with limited effective therapies. Nanotechnology-based drug delivery systems offer targeted and efficient treatment strategies.Objective: This study aimed to develop chitosan-coated gold nanoparticles loaded with Nobiletin (Ch-AuNPs-NB) and evaluate their anticancer potential by targeting the PI3K/AKT/mTOR signaling pathway in GC.Materials and MethodsCh-AuNPs were synthesized by NaBH4 reduction and loaded with Nobiletin using nanoprecipitation. Characterization was done using UV-Vis, FTIR, XRD, DLS, and TEM. Drug loading, encapsulation efficiency, and pH-responsive release were assessed.Results: Ch-AuNPs-NB (∼120 nm, PDI 0.247, +51 mV) showed enhanced drug loading (15%) and encapsulation efficiency (90.4%) at higher NB concentrations. The formulation demonstrated pH-responsive release over 72 hours and stability for 60 days.Discussion and conclusion: Ch-AuNPs-NB inhibited the PI3K/AKT/mTOR pathway, induced apoptosis, and arrested the cell cycle in AGS cells, highlighting its potential as a targeted GC therapy.","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"1-15"},"PeriodicalIF":3.0000,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The pH-controlled release properties of chitosan-gold nanoparticles encapsulated nobiletin to induce apoptosis through PI3K/AKT/mTOR signalling pathway on gastric cancer cell lines.\",\"authors\":\"Hongyi Qing, Ping Xie, Zhenjiang Wu, Wenhai Fan, Yuanxiao Liang, Xiulian Xu\",\"doi\":\"10.1080/02652048.2025.2507638\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Context: Gastric cancer (GC) remains a major health concern with limited effective therapies. Nanotechnology-based drug delivery systems offer targeted and efficient treatment strategies.Objective: This study aimed to develop chitosan-coated gold nanoparticles loaded with Nobiletin (Ch-AuNPs-NB) and evaluate their anticancer potential by targeting the PI3K/AKT/mTOR signaling pathway in GC.Materials and MethodsCh-AuNPs were synthesized by NaBH4 reduction and loaded with Nobiletin using nanoprecipitation. Characterization was done using UV-Vis, FTIR, XRD, DLS, and TEM. Drug loading, encapsulation efficiency, and pH-responsive release were assessed.Results: Ch-AuNPs-NB (∼120 nm, PDI 0.247, +51 mV) showed enhanced drug loading (15%) and encapsulation efficiency (90.4%) at higher NB concentrations. The formulation demonstrated pH-responsive release over 72 hours and stability for 60 days.Discussion and conclusion: Ch-AuNPs-NB inhibited the PI3K/AKT/mTOR pathway, induced apoptosis, and arrested the cell cycle in AGS cells, highlighting its potential as a targeted GC therapy.\",\"PeriodicalId\":16391,\"journal\":{\"name\":\"Journal of microencapsulation\",\"volume\":\" \",\"pages\":\"1-15\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-05-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of microencapsulation\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/02652048.2025.2507638\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microencapsulation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02652048.2025.2507638","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

摘要

背景：胃癌（GC）仍然是一个主要的健康问题，有效的治疗方法有限。基于纳米技术的药物输送系统提供了有针对性和有效的治疗策略。目的：制备壳聚糖包被的Nobiletin负载金纳米颗粒（Ch-AuNPs-NB），并通过GC中PI3K/AKT/mTOR信号通路评价其抗癌潜力。材料与方法采用NaBH4还原法制备sch - aunps，并采用纳米沉淀法负载诺比列素。采用UV-Vis， FTIR， XRD， DLS和TEM进行表征。评估药物负荷、包封效率和ph响应释放。结果：Ch-AuNPs-NB （~ 120 nm, PDI 0.247, +51 mV）在较高的NB浓度下，载药量（15%）和包封率（90.4%）增强。该制剂具有72小时的ph响应释放和60天的稳定性。讨论与结论：Ch-AuNPs-NB在AGS细胞中抑制PI3K/AKT/mTOR通路，诱导细胞凋亡，阻滞细胞周期，具有靶向治疗胃癌的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

The pH-controlled release properties of chitosan-gold nanoparticles encapsulated nobiletin to induce apoptosis through PI3K/AKT/mTOR signalling pathway on gastric cancer cell lines.

Context: Gastric cancer (GC) remains a major health concern with limited effective therapies. Nanotechnology-based drug delivery systems offer targeted and efficient treatment strategies.

Objective: This study aimed to develop chitosan-coated gold nanoparticles loaded with Nobiletin (Ch-AuNPs-NB) and evaluate their anticancer potential by targeting the PI3K/AKT/mTOR signaling pathway in GC.Materials and MethodsCh-AuNPs were synthesized by NaBH₄ reduction and loaded with Nobiletin using nanoprecipitation. Characterization was done using UV-Vis, FTIR, XRD, DLS, and TEM. Drug loading, encapsulation efficiency, and pH-responsive release were assessed.

Results: Ch-AuNPs-NB (∼120 nm, PDI 0.247, +51 mV) showed enhanced drug loading (15%) and encapsulation efficiency (90.4%) at higher NB concentrations. The formulation demonstrated pH-responsive release over 72 hours and stability for 60 days.

Discussion and conclusion: Ch-AuNPs-NB inhibited the PI3K/AKT/mTOR pathway, induced apoptosis, and arrested the cell cycle in AGS cells, highlighting its potential as a targeted GC therapy.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.