吸烟对口腔黏膜SARS-CoV-2感染相关分子表达的影响

IF 0.4 Q4 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral and Maxillofacial Surgery Medicine and Pathology Pub Date : 2025-02-04 DOI:10.1016/j.ajoms.2025.01.016

Toshio Yudo , Reiko Tokuyama-Toda , Shinji Ide , Yusuke Takebe , Mai Takeshita-Umehara , Kohei Ijichi , Misao Ishikawa , Kazuhito Satomura

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引用次数: 0

摘要

目的探讨包括ACE2在内的多种SARS-CoV-2感染相关分子是否在口腔组织中表达，并阐明吸烟对这些分子的影响。方法采用免疫组化方法检测正常口腔黏膜和唾液腺组织中SARS-CoV-2感染相关分子ACE2、NRP-1、TMPRSS和Furin的表达。本研究还研究了香烟烟雾提取物（CSE）体外作用于口腔粘膜上皮细胞时SARS-CoV-2感染相关分子的表达情况，并检测了这些分子在吸烟者和非吸烟者口腔组织中mRNA和蛋白水平的表达差异。结果ACE2、NRP-1、TMPRSS2、Furin在口腔黏膜组织和唾液腺组织中均有表达。当口腔粘膜上皮细胞暴露于CSE时，ACE2和Furin的mRNA表达呈剂量和时间依赖性增加，而NRP-1的表达增加到1 % CSE，但减少到5 % CSE。在蛋白质表达方面，只有NRP-1增加到1 % CSE，其mRNA也增加了。这些分子在吸烟者和非吸烟者口腔黏膜组织中的表达倾向于吸烟者较高；然而，差异并不显著。结论SARS-CoV-2感染相关分子ACE2、NRP-1、TMPRSS和Furin在口腔黏膜和唾液腺组织中表达。这些分子可能会受到吸烟的影响，表明口腔健康的重要性，并被认为是未来反吸烟运动的科学依据之一。数据可用性声明支持本研究结果的数据可根据通讯作者的合理要求提供。

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Effects of smoking on the expression of SARS-CoV-2 infection-related molecules in the oral mucosa

Objective

This study aimed to investigate whether various SARS-CoV-2 infection-related molecules, including ACE2, are expressed in oral tissues and clarify the effects of smoking on these.

Methods

The expressions of the SARS-CoV-2 infection-related molecules ACE2, NRP-1, TMPRSS, and Furin in normal oral mucosa and salivary gland tissues were immunohistochemically examined. This study also investigated the expression of SARS-CoV-2 infection-related molecules when cigarette smoke extract (CSE) was applied to oral mucosal epithelial cells in vitro and examined differences in the expression of these molecules at the mRNA and protein levels in the oral tissues of smokers and nonsmokers.

Results

The expression of ACE2, NRP-1, TMPRSS2, and Furin was observed in the oral mucosal tissues and salivary gland tissues. When oral mucosal epithelial cells were exposed to CSE, the mRNA expression of ACE2 and Furin increased in a dose- and time-dependent manner, whereas the expression of NRP-1 increased up to 1 % CSE but decreased to 5 % CSE. In terms of protein expression, only NRP-1 increased up to 1 % CSE, as did its mRNA. The expression of these molecules in the oral mucosal tissues of smokers and nonsmokers tended to be higher in smokers; however, the difference was not significant.

Conclusions

This study revealed that the SARS-CoV-2 infection-related molecules ACE2, NRP-1, TMPRSS, and Furin are expressed in the oral mucosa and salivary gland tissue. These molecules may be affected by smoking and indicate the importance of oral health and are thought to be one of the scientific grounds for future antismoking campaigns.