Elena Hakme , Sussie Pagh , Victor Manuel Cutillas , Sara Haydar , Mette Sif Hansen , Hanne Lyngholm Larsen , Peter Roslev , Mette Erecius Poulsen
{"title":"丹麦农业区欧洲野兔(Lepus europaeus)暴露于杀虫剂","authors":"Elena Hakme , Sussie Pagh , Victor Manuel Cutillas , Sara Haydar , Mette Sif Hansen , Hanne Lyngholm Larsen , Peter Roslev , Mette Erecius Poulsen","doi":"10.1016/j.envpol.2025.126597","DOIUrl":null,"url":null,"abstract":"<div><div>There has been a decline in the population of European hare (<em>Lepus europaeus</em>) throughout Europe. This is considered to be due to the intensified industrial agriculture and the loss of native biotopes. Exposure to pesticides has also been mentioned as a reason for reduced reproduction and population decline. The objective of this study was to assess the concentrations and types of pesticides found in European hares in Denmark. A total of 48 lung samples, 51 pelt samples, 54 urine samples, and 100 liver samples were analyzed using multi-residue and specific sample preparation methods, followed by LC-MS/MS and GC-MS/MS. In these samples, 32 pesticides/metabolites were detected: 20 in pelt, 12 in urine, 11 in liver, and five in lung. The most frequently detected pesticides in the pelt were tebuconazole, boscalid, and prosulfocarb, in urine it was the herbicide glyphosate, its metabolite AMPA, and boscalid, and in the liver the most frequently detected pesticide was metrafenone. The most frequently found pesticide in lung samples was spirotetramat-enol glucoside, a metabolite of spirotetramat, though it was present in only a few hares (6 %). Geometric means of detected pesticides were generally below 100 μg/kg: in pelt (4.59–67.4 μg/kg), in urine (4.79–195 μg/mL), in liver (5.58–85.8 μg/kg), and in lung (8.35–26.6 μg/kg). The high absolute number of pesticides in the pelt suggests exposure through contact with newly sprayed fields. Although concentrations of pesticides were generally low, it cannot be excluded that pesticides act additively in combination to other environmental challenges and thereby in the long run may have an effect on the physiology and life span of hares.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"381 ","pages":"Article 126597"},"PeriodicalIF":7.3000,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exposure of European hares (Lepus europaeus) to pesticides in Danish agricultural areas\",\"authors\":\"Elena Hakme , Sussie Pagh , Victor Manuel Cutillas , Sara Haydar , Mette Sif Hansen , Hanne Lyngholm Larsen , Peter Roslev , Mette Erecius Poulsen\",\"doi\":\"10.1016/j.envpol.2025.126597\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>There has been a decline in the population of European hare (<em>Lepus europaeus</em>) throughout Europe. This is considered to be due to the intensified industrial agriculture and the loss of native biotopes. Exposure to pesticides has also been mentioned as a reason for reduced reproduction and population decline. The objective of this study was to assess the concentrations and types of pesticides found in European hares in Denmark. A total of 48 lung samples, 51 pelt samples, 54 urine samples, and 100 liver samples were analyzed using multi-residue and specific sample preparation methods, followed by LC-MS/MS and GC-MS/MS. In these samples, 32 pesticides/metabolites were detected: 20 in pelt, 12 in urine, 11 in liver, and five in lung. The most frequently detected pesticides in the pelt were tebuconazole, boscalid, and prosulfocarb, in urine it was the herbicide glyphosate, its metabolite AMPA, and boscalid, and in the liver the most frequently detected pesticide was metrafenone. The most frequently found pesticide in lung samples was spirotetramat-enol glucoside, a metabolite of spirotetramat, though it was present in only a few hares (6 %). Geometric means of detected pesticides were generally below 100 μg/kg: in pelt (4.59–67.4 μg/kg), in urine (4.79–195 μg/mL), in liver (5.58–85.8 μg/kg), and in lung (8.35–26.6 μg/kg). The high absolute number of pesticides in the pelt suggests exposure through contact with newly sprayed fields. Although concentrations of pesticides were generally low, it cannot be excluded that pesticides act additively in combination to other environmental challenges and thereby in the long run may have an effect on the physiology and life span of hares.</div></div>\",\"PeriodicalId\":311,\"journal\":{\"name\":\"Environmental Pollution\",\"volume\":\"381 \",\"pages\":\"Article 126597\"},\"PeriodicalIF\":7.3000,\"publicationDate\":\"2025-06-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Pollution\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0269749125009704\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Pollution","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0269749125009704","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Exposure of European hares (Lepus europaeus) to pesticides in Danish agricultural areas
There has been a decline in the population of European hare (Lepus europaeus) throughout Europe. This is considered to be due to the intensified industrial agriculture and the loss of native biotopes. Exposure to pesticides has also been mentioned as a reason for reduced reproduction and population decline. The objective of this study was to assess the concentrations and types of pesticides found in European hares in Denmark. A total of 48 lung samples, 51 pelt samples, 54 urine samples, and 100 liver samples were analyzed using multi-residue and specific sample preparation methods, followed by LC-MS/MS and GC-MS/MS. In these samples, 32 pesticides/metabolites were detected: 20 in pelt, 12 in urine, 11 in liver, and five in lung. The most frequently detected pesticides in the pelt were tebuconazole, boscalid, and prosulfocarb, in urine it was the herbicide glyphosate, its metabolite AMPA, and boscalid, and in the liver the most frequently detected pesticide was metrafenone. The most frequently found pesticide in lung samples was spirotetramat-enol glucoside, a metabolite of spirotetramat, though it was present in only a few hares (6 %). Geometric means of detected pesticides were generally below 100 μg/kg: in pelt (4.59–67.4 μg/kg), in urine (4.79–195 μg/mL), in liver (5.58–85.8 μg/kg), and in lung (8.35–26.6 μg/kg). The high absolute number of pesticides in the pelt suggests exposure through contact with newly sprayed fields. Although concentrations of pesticides were generally low, it cannot be excluded that pesticides act additively in combination to other environmental challenges and thereby in the long run may have an effect on the physiology and life span of hares.
期刊介绍:
Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health.
Subject areas include, but are not limited to:
• Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies;
• Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change;
• Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects;
• Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects;
• Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest;
• New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.