几丁质和层粘连素引发植物对大黄豆锈病的防御反应

IF 3.1 2区 农林科学 Q2 PLANT SCIENCES
Shuxian Li, Nicholas Rhoades, Jodi Scheffler, Guixia Hao
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引用次数: 0

摘要

大豆(甘氨酸max (l))小麦是世界上最重要的经济作物之一。大豆锈病等多种病害严重影响大豆生产。激发子处理已被用于提高植物对多种疾病的抗性。为了研究激发子处理是否能诱导大豆抗性,本研究通过中试试验研究了激发子(几丁质、层状蛋白以及与两者共处理)对5种大豆基因型活性氧(ROS)爆发的影响。我们发现,所有刺激剂处理均诱导不同程度的ROS爆发。诱导子处理后,几种植物防御基因的表达上调。GmCERK1、GmRbohD、GmPR1、GmPR2、GmPAL和GmCHS在诱导子处理后3 h表达量最高。有趣的是,几丁质和海带动物联合处理显著增强了GmPAL和GmCHS的表达。采用诱导剂处理大豆锈病的方法,对接种粗根Phakopsora前的大豆锈病严重程度进行了评价。采用五分制,最高为5分。几丁质处理后,Williams 82和PI 200526的严重程度分别降至2.0和1.9。未启动子处理的对照组为4.2和3.8,显著高于启动子处理的基因型(p < 0.001)。据我们所知,这是第一次证明激发子几丁质和层粘连蛋白在诱导大豆抗厚根菌感染方面的作用。本研究结果对开发大豆锈病或其他作物病害的防治方法具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chitin and Laminarin Trigger Plant Defense Responses Against Soybean Rust Caused by Phakopsora pachyrhizi.

Soybean (Glycine max) is one of the most economically important crops in the world. Production of soybean can be severely impacted by many diseases, including soybean rust. Elicitor treatments have been utilized to enhance plant resistance against multiple diseases. To investigate whether elicitor treatment can induce soybean resistance, pilot experiments were conducted to test the effects of elicitors (chitin, laminarin, and co-treated with both) on the reactive oxygen species (ROS) burst in five soybean genotypes. We discovered that all elicitor treatments induced an ROS burst with different levels. The expression of several plant defense genes was upregulated in soybean Williams 82 following elicitor treatments. GmCERK1, GmRbohD, GmPR1, GmPR2, GmPAL, and GmCHS exhibited the highest expression at 3 h post-elicitor treatments. Interestingly, co-treatment with chitin and laminarian significantly enhanced the expression of GmPAL and GmCHS. Soybean rust severity was evaluated on plants with elicitor treatment prior to Phakopsora pachyrhizi inoculation. A 5-point scale, with 5 as the highest, was used. With chitin treatment, the severities were reduced to 2.0 and 1.9 in Williams 82 and PI 200526, respectively. Controls without elicitor treatments had severities of 4.2 and 3.8, which were significantly (P < 0.001) higher than the severities in the genotypes with elicitor treatments. To the best of our knowledge, this is the first demonstration of the effects of elicitors chitin and laminarin on inducing resistance in soybean against P. pachyrhizi infection. The information from this research will be useful for development of an alternative method to control soybean rust or other diseases in crops.

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来源期刊
Phytopathology
Phytopathology 生物-植物科学
CiteScore
5.90
自引率
9.40%
发文量
505
审稿时长
4-8 weeks
期刊介绍: Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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