Ag₂-固定化枯草芽孢杆菌透明质酸的协同光催化和生物医学应用。

IF 4.9 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Sikander Ali, Hijab Zahra, Muhammad Usman Ahmad, Amany A Abdel-Rheem, Muhammad Afzaal, Rab Nawaz, Bakar Bin Khatab Abbasi, Ali Irfan, Yousef A Bin Jardan
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引用次数: 0

摘要

本研究报道了利用枯草芽孢杆菌PV154141.1合成透明质酸包被氧化银(Ag2O)纳米吸附剂的环境和生物医学双重应用。对硝酸银(AgNO3)浓度和反应时间等参数对HA-Ag2O纳米吸附剂的形成和稳定性进行了优化。采用x射线衍射(XRD)分析、扫描电镜(SEM)和能量色散x射线(EDX)光谱等表征技术对合成的HA-Ag2O纳米吸附剂的结构性能、形貌和元素组成进行了表征。XRD谱图证实了Ag2O纳米吸附剂的存在,峰的hkl值分别为110、111、200、220、311和222,分别位于2θ约为26.46°、32.55°、37.76°、54.48°、64.92°和68.19°的位置。SEM分析表明,纳米吸附剂的平均粒径为193.93±0.23 nm, EDX分析证实了其元素组成。优化后的纳米吸附剂进行了各种应用,包括抗菌活性、抗氧化活性和光催化染料降解效率。对HA-Ag2O纳米吸附剂对革兰氏阴性(大肠杆菌)和革兰氏阳性(金黄色葡萄球菌、乳杆菌)病原菌的抑菌活性进行了评价,显示出广谱抗菌效果。对金黄色葡萄球菌的抑菌活性最高(8±0.4 mm),提示其具有治疗耐药感染的潜力。此外,使用2,2-二苯基-1-吡啶肼(DPPH)测定法评估的抗氧化能力显示,自由基清除活性为84.36±0.44%。在紫外(λ = 365 nm)照射下,HA-Ag2O纳米吸附剂在120 min内对亚甲基蓝(MB)染料的降解率达到82.28%,表现出较强的光催化活性。与传统的Ag2O系统不同,这些生物杂化纳米吸附剂将Ag2O的光催化效率与微生物衍生的HA的生物相容性和生物膜抑制特性结合在一起,实现了单组分系统无法实现的双重功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synergistic photocatalytic and biomedical applications of Ag₂O-immobilized Bacillus subtilis-hyaluronic acid.

This study reports the synthesis of hyaluronic acid-coated silver oxide (Ag2O) nano-adsorbents using Bacillus subtilis PV154141.1 for dual environmental and biomedical applications. Multiple parameters were optimized for the formation and constancy of HA-Ag2O nano-adsorbents, including silver nitrate (AgNO3) concentration and reaction time. Several characterization techniques including X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy were employed to determine the structural properties, morphology, and elemental composition of the synthesized HA-Ag2O nano-adsorbents. XRD pattern confirmed the presence of Ag2O nano-adsorbents by showing peaks having hkl values of 110, 111, 200, 220, 311, and 222, located at 2θ values of roughly 26.46°, 32.55°, 37.76°, 54.48°, 64.92° and 68.19°, respectively. SEM analysis indicated a mean particle size of 193.93 ± 0.23 nm for the nano-adsorbents, while EDX confirmed their elemental composition. The optimized nano-adsorbents were subjected to various applications, including the antibacterial activity, antioxidant activity, and photocatalytic dye degradation efficiency. The antibacterial activity of HA-Ag2O nano-adsorbents was evaluated against gram-negative (E. coli) and gram-positive (S. aureus, Lactobacillus spp.) pathogens, demonstrating broad-spectrum efficacy. The highest activity was observed against S. aureus (8 ± 0.4 mm inhibition zone), underscoring their potential to treat drug-resistent infections. Additionally, the antioxidant capacity, evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, exhibited a radical scavenging activity of 84.36 ± 0.44%. Under UV irradiation (λ = 365 nm), the HA-Ag2O nano-adsorbents achieved 82.28% degradation of methylene blue (MB) dye within 120 min, demonstrating robust photocatalytic activity. Unlike conventional Ag2O systems, these biohybrid nano-adsorbents combine the photocatalytic efficiency of Ag2O with the biocompatibility and biofilm-inhibiting properties of microbially-derived HA, enabling dual functionality not achieved in single-component systems.

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来源期刊
Microbial Cell Factories
Microbial Cell Factories 工程技术-生物工程与应用微生物
CiteScore
9.30
自引率
4.70%
发文量
235
审稿时长
2.3 months
期刊介绍: Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology. The journal is divided into the following editorial sections: -Metabolic engineering -Synthetic biology -Whole-cell biocatalysis -Microbial regulations -Recombinant protein production/bioprocessing -Production of natural compounds -Systems biology of cell factories -Microbial production processes -Cell-free systems
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