利用生物纳米孔测定DNA甲基化和碱基修饰的位置。

IF 10.7 2区材料科学 Q1 CHEMISTRY, PHYSICAL

Small Methods Pub Date : 2025-06-04 DOI:10.1002/smtd.202401760

Ping Liu, Masayuki Honda, Ryuji Kawano

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引用次数: 0

摘要

开发了一种利用生物纳米孔检测DNA甲基化和修饰的方法。通过利用α-溶血素纳米孔入口和颈部区域的碱基与酸性氨基酸之间的相互作用，我们确定了5-甲基胞嘧啶在寡核苷酸中的位置和频率。此外，通过检测电解质中不同的离子种类和浓度，优化了去甲基化中间体的检测。人们还努力采用商用纳米孔设备进行高通量检测。这种方法提供了使用生物纳米孔直接检测DNA甲基化和修饰的潜力。

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Determination of the position of DNA Methylation and Base Modifications Using a Biological Nanopore.

A method for detecting DNA methylation and modifications is developed using biological nanopores. By exploiting the interaction between bases and acidic amino acids within the entrance and neck region of the α-hemolysin nanopore, we determined the position and frequency of 5-methylcytosine in oligonucleotides. Furthermore, the detection of demethylation intermediates is optimized by examining various ion species and concentrations in the electrolyte. Efforts are also made to employ commercial nanopore devices for high-throughput detection. This approach offers the potential for direct detection of DNA methylation and modifications using biological nanopores.

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来源期刊

Small Methods Materials Science-General Materials Science

CiteScore

17.40

自引率

1.60%

发文量

347

期刊介绍： Small Methods is a multidisciplinary journal that publishes groundbreaking research on methods relevant to nano- and microscale research. It welcomes contributions from the fields of materials science, biomedical science, chemistry, and physics, showcasing the latest advancements in experimental techniques. With a notable 2022 Impact Factor of 12.4 (Journal Citation Reports, Clarivate Analytics, 2023), Small Methods is recognized for its significant impact on the scientific community. The online ISSN for Small Methods is 2366-9608.