虫媒线虫与噻虫嗪对小蠹蛾成熟幼虫的协同作用

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-06-02 DOI:10.1016/j.cropro.2025.107305

Haibin Wu , Ganyu Zhang , Yi Zhu , Yong Zhang , Tingting Liu , Mian Wang , Kang Qiao , Lili Jiang

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引用次数: 0

摘要

桃果蛾，Carposina sasakii，是梨果和核果的持续威胁。本研究以7种昆虫病原线虫（Heterorhabditis bacteriophora H06 （H06）、carpocapae All （All）、feltima JY-90 （JY-90）、S. carpocapae NC116 （NC116）、stinema longicaudum X-7 （X-7）、S. feltiae Sf - sn （Sf）和Heterorhabditis indica LN2 (LN2)）为研究对象，测定了它们对sasakii成年幼虫的生物毒性。在此基础上，进一步评价了温度和土壤湿度对三种最有效的EPNs与噻虫嗪联合施用后的sasakii成熟幼虫死亡率的影响。在室内和田间条件下，采用效果最好的H06与噻虫嗪进行协同防治。结果表明，H06、LN2和X-7对佐氏弓形虫成熟幼虫的死亡率较高，且在实验室条件下，3种EPNs剂量为20亿条/ha与1.5 kg/ha噻虫嗪之间存在加性效应。在20℃、基质含水量为10 ~ 18%的条件下，H06与噻虫嗪的致死率高于X-7和LN2。H06在10 ~ 15亿IJs/ha和噻虫嗪在0.5 ~ 0.75 kg/ha之间存在协同效应。在田间试验中，10亿IJs/公顷的H06 + 0.5 kg/公顷的噻虫嗪两次施用比30亿IJs/公顷的H06 + 1.5 kg/公顷的噻虫嗪单次施用的防治效果更好。与传统氯虫腈处理相比，H06 +噻虫嗪的防治效果最好，3个月的最低防治效果为93.08%。综上所述，这一新型技术将传统的化学防治方法由对成虫、虫卵和新孵化的幼虫的常规喷雾方法转变为生物剂和化学剂的协同组合，对土壤下的佐佐氏弧菌成熟幼虫进行防治。结果表明，土壤中成熟幼虫的防治技术是一种很有前途的方法，可纳入水果生产的综合管理方案。

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Synergistic interactions between entomopathogenic nematodes and thiamethoxam to manage mature larvae of Carposina sasakii (Lepidoptera: Carposinidae)

The peach fruit moth, Carposina sasakii, is a constant threat to pome and stone fruits. In this study, seven entomopathogenic nematode species (EPNs), including Heterorhabditis bacteriophora H06 (H06), Steinernema carpocapsae All (All), Steinernema feltiae JY-90 (JY-90), S. carpocapsae NC116 (NC116), Steinernema longicaudum X-7 (X-7), S. feltiae SF-SN (Sf) and Heterorhabditis indica LN2 (LN2) were tested for biotoxicity against C. sasakii mature larvae. Then the effects of temperature and soil moisture on the mortality of C. sasakii mature larvae caused by the three most efficient EPNs combined with thiamethoxam were further evaluated. Moreover, H06, the most effective species, was employed to determine the synergistic effects with thiamethoxam to control C. sasakii under laboratory and field conditions. Results showed that H06, LN2 and X-7 caused high mortality of C. sasakii mature larvae, and an additive effect was found between the three EPNs at 2.0 billion infective juveniles (IJs)/ha and thiamethoxam at 1.5 kg/ha under laboratory conditions. In combination with thiamethoxam, H06 caused higher mortality than X-7 and LN2 at 20 °C and substrate moisture of 10–18 %. Synergism was observed between H06 at 1.0 and 1.5 billion IJs/ha and thiamethoxam at 0.5 and 0.75 kg/ha. In the field trials, H06 at 1.0 billion IJs/ha + thiamethoxam at 0.5 kg/ha applied twice had greater control effects than that of a single treatment with H06 at 3.0 billion IJs/ha + thiamethoxam at 1.5 kg/ha. Compared to traditional chlorantraniliprole treatment, H06 + thiamethoxam displayed the greatest control effect, which reached a minimum control efficacy of 93.08 % for up to three months. Taken together, this new type technology transformed traditional chemical control using conventional spray method against adults, eggs and newly hatched larvae into the synergistic combination of a biological agent and a chemical agent against C. sasakii mature larvae under the soil. The results suggested that the technique to control C. sasakii mature larvae in soil was a promising method to be incorporated in the integrated management programs in fruit production.

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.