衰老会改变人类真皮成纤维细胞中锌、镁和白细胞介素-8的水平

IF 3.6 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Trace Elements in Medicine and Biology Pub Date : 2025-06-01 DOI:10.1016/j.jtemb.2025.127673

Nishat Zakaria , Matthew J. Smith , David Messenger , Ranjit Bhogal , Richard Siow , Wolfgang Maret

{"title":"衰老会改变人类真皮成纤维细胞中锌、镁和白细胞介素-8的水平","authors":"Nishat Zakaria , Matthew J. Smith , David Messenger , Ranjit Bhogal , Richard Siow , Wolfgang Maret","doi":"10.1016/j.jtemb.2025.127673","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Ageing is linked to a decline in cellular functions, weakening the skin’s defence mechanisms. At advanced ages, deficiencies in micronutrients like zinc and magnesium are common. Zinc deficiency from inadequate intake or absorption can lead to skin issues such as delayed wound healing and eczema. Dysregulated zinc homeostasis can increase the risk of dermatitis and psoriasis.</div></div><div><h3>Objectives</h3><div>This study examined the relationship between zinc and magnesium levels and skin ageing and inflammation in human dermal fibroblasts from young and old donors.</div></div><div><h3>Methods</h3><div>Primary dermal fibroblasts from young (20–30 years) and older (62–75 years) donors were assessed. We evaluated oxidative stress, determined susceptibility to senescence, and analysed gene expression profiles while quantifying the secretion of the chemokine interleukin-8. Markers of zinc homeostasis were evaluated by assessing protein expression via western blotting, and both free zinc ions and total metal ion concentrations were quantified.</div></div><div><h3>Results</h3><div>Older fibroblasts showed increased oxidative stress and higher susceptibility to senescence. Interleukin-6 expression rose in aged cells, while interleukin-8 secretion declined significantly. Free zinc ion concentrations and zinc transporter 1 expression were elevated, along with total zinc and magnesium concentrations.</div></div><div><h3>Conclusions</h3><div>Reduced interleukin-8 secretion compromises immune regulation in ageing skin. Elevated free zinc ion levels and zinc transporter expression contribute to eventual cellular zinc loss, impairing inflammatory responses and weakening immunity. These findings highlight the critical role of zinc and magnesium micronutrient balance in maintaining skin health during ageing.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127673"},"PeriodicalIF":3.6000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ageing alters zinc, magnesium, and interleukin-8 levels in human dermal fibroblasts\",\"authors\":\"Nishat Zakaria , Matthew J. Smith , David Messenger , Ranjit Bhogal , Richard Siow , Wolfgang Maret\",\"doi\":\"10.1016/j.jtemb.2025.127673\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Ageing is linked to a decline in cellular functions, weakening the skin’s defence mechanisms. At advanced ages, deficiencies in micronutrients like zinc and magnesium are common. Zinc deficiency from inadequate intake or absorption can lead to skin issues such as delayed wound healing and eczema. Dysregulated zinc homeostasis can increase the risk of dermatitis and psoriasis.</div></div><div><h3>Objectives</h3><div>This study examined the relationship between zinc and magnesium levels and skin ageing and inflammation in human dermal fibroblasts from young and old donors.</div></div><div><h3>Methods</h3><div>Primary dermal fibroblasts from young (20–30 years) and older (62–75 years) donors were assessed. We evaluated oxidative stress, determined susceptibility to senescence, and analysed gene expression profiles while quantifying the secretion of the chemokine interleukin-8. Markers of zinc homeostasis were evaluated by assessing protein expression via western blotting, and both free zinc ions and total metal ion concentrations were quantified.</div></div><div><h3>Results</h3><div>Older fibroblasts showed increased oxidative stress and higher susceptibility to senescence. Interleukin-6 expression rose in aged cells, while interleukin-8 secretion declined significantly. Free zinc ion concentrations and zinc transporter 1 expression were elevated, along with total zinc and magnesium concentrations.</div></div><div><h3>Conclusions</h3><div>Reduced interleukin-8 secretion compromises immune regulation in ageing skin. Elevated free zinc ion levels and zinc transporter expression contribute to eventual cellular zinc loss, impairing inflammatory responses and weakening immunity. These findings highlight the critical role of zinc and magnesium micronutrient balance in maintaining skin health during ageing.</div></div>\",\"PeriodicalId\":49970,\"journal\":{\"name\":\"Journal of Trace Elements in Medicine and Biology\",\"volume\":\"89 \",\"pages\":\"Article 127673\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2025-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Trace Elements in Medicine and Biology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0946672X25000860\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Trace Elements in Medicine and Biology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0946672X25000860","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

衰老与细胞功能下降有关，削弱了皮肤的防御机制。在老年，缺乏锌和镁等微量营养素是很常见的。锌的摄入或吸收不足会导致皮肤问题，如伤口愈合延迟和湿疹。锌体内平衡失调会增加患皮炎和牛皮癣的风险。目的探讨锌和镁水平与皮肤老化和炎症之间的关系。方法对年轻（20 ~ 30岁）和老年（62 ~ 75岁）供者的真皮成纤维细胞进行评价。我们评估了氧化应激，确定了衰老的易感性，并分析了基因表达谱，同时量化了趋化因子白介素-8的分泌。western blotting检测蛋白表达，定量测定游离锌离子和总金属离子浓度。结果成纤维细胞氧化应激增强，衰老易感性增强。衰老细胞中白细胞介素-6表达升高，白细胞介素-8分泌明显下降。游离锌离子浓度和锌转运蛋白1的表达随着总锌和总镁浓度的升高而升高。结论皮肤老化导致白细胞介素-8分泌减少，影响免疫调节。游离锌离子水平升高和锌转运蛋白表达导致最终的细胞锌流失，损害炎症反应和削弱免疫力。这些发现强调了锌和镁微量营养素平衡在维持衰老期间皮肤健康方面的关键作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Ageing alters zinc, magnesium, and interleukin-8 levels in human dermal fibroblasts

Background

Ageing is linked to a decline in cellular functions, weakening the skin’s defence mechanisms. At advanced ages, deficiencies in micronutrients like zinc and magnesium are common. Zinc deficiency from inadequate intake or absorption can lead to skin issues such as delayed wound healing and eczema. Dysregulated zinc homeostasis can increase the risk of dermatitis and psoriasis.

Objectives

This study examined the relationship between zinc and magnesium levels and skin ageing and inflammation in human dermal fibroblasts from young and old donors.

Methods

Primary dermal fibroblasts from young (20–30 years) and older (62–75 years) donors were assessed. We evaluated oxidative stress, determined susceptibility to senescence, and analysed gene expression profiles while quantifying the secretion of the chemokine interleukin-8. Markers of zinc homeostasis were evaluated by assessing protein expression via western blotting, and both free zinc ions and total metal ion concentrations were quantified.

Results

Older fibroblasts showed increased oxidative stress and higher susceptibility to senescence. Interleukin-6 expression rose in aged cells, while interleukin-8 secretion declined significantly. Free zinc ion concentrations and zinc transporter 1 expression were elevated, along with total zinc and magnesium concentrations.

Conclusions

Reduced interleukin-8 secretion compromises immune regulation in ageing skin. Elevated free zinc ion levels and zinc transporter expression contribute to eventual cellular zinc loss, impairing inflammatory responses and weakening immunity. These findings highlight the critical role of zinc and magnesium micronutrient balance in maintaining skin health during ageing.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Trace Elements in Medicine and Biology 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.90%

发文量

202

审稿时长

85 days

期刊介绍： The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.