基于高效液相色谱的碳水化合物自动合成和纯化的强化实验装置

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2025-05-27 DOI:10.1016/j.carres.2025.109554

Gustavo A. Kashiwagi, Alexei V. Demchenko

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引用次数: 0

摘要

本文报道了一种改进的基于高效液相色谱的自动（HPLC-A）合成器，能够在温度控制下合成和纯化碳水化合物。开发的平台允许进行各种保护基团操作，糖基受体的合成，以及引入三氯乙酸酯离去基。展示了一种全自动合成和纯化不同单糖的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Enhanced experimental set-up for HPLC-based automated synthesis and purification of carbohydrate building blocks

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Enhanced experimental set-up for HPLC-based automated synthesis and purification of carbohydrate building blocks

Reported herein is an improved HPLC-based automated (HPLC-A) synthesizer capable of temperature-controlled synthesis and purification of carbohydrates. The developed platform allows to perform various protecting group manipulations, synthesis of glycosyl acceptors, as well as introduction of the trichloroacetimidate leaving group. A fully automated synthesis and purification was showcased in application to different monosaccharides.

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".