{"title":"绘制斑马鱼再生心脏图","authors":"Alexandra Le Bras","doi":"10.1038/s41684-025-01569-6","DOIUrl":null,"url":null,"abstract":"<p>While adult mammals have an extremely limited capacity to repair lost or damaged heart tissue, many adult fish, including zebrafish, can successfully regenerate their hearts after injury. Uncovering the mechanisms of zebrafish heart regeneration could open new avenues to regenerate human hearts and prevent heart failure. In a new study, researchers combined the use of spatial transcriptomics (Stereo-seq) and single-cell RNA-sequencing (scRNA-seq) to generate a spatially-resolved molecular and cellular atlas of the regenerating zebrafish heart across eight stages, including pre-injury, 6 hours post-amputation (hpa) of the ventricular apex, 12 hpa, 1 day post-amputation (dpa), 3 dpa, 7 dpa, 14 dpa, and 28 dpa. Using this comprehensive atlas, they characterized the dynamic changes in cell type composition throughout cardiac regeneration, including proliferating macrophages, pro-regenerative fibroblasts and regenerating cardiomyocytes. The study also identified <i>tpm4a</i> as a critical gene for cardiomyocyte re-differentiation during the regeneration process. Finally, the researchers created a ‘virtual regenerating heart’, encompassing both spatial information and time, which can be accessed here: https://db.cngb.org/stomics/zebrafish_VRH/</p><p><b>Original reference:</b> Li, L. et al. <i>Nat. Commun</i>. <b>16</b>, 3716 (2025)</p>","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"260 1","pages":""},"PeriodicalIF":5.9000,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mapping the zebrafish regenerative heart\",\"authors\":\"Alexandra Le Bras\",\"doi\":\"10.1038/s41684-025-01569-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>While adult mammals have an extremely limited capacity to repair lost or damaged heart tissue, many adult fish, including zebrafish, can successfully regenerate their hearts after injury. Uncovering the mechanisms of zebrafish heart regeneration could open new avenues to regenerate human hearts and prevent heart failure. In a new study, researchers combined the use of spatial transcriptomics (Stereo-seq) and single-cell RNA-sequencing (scRNA-seq) to generate a spatially-resolved molecular and cellular atlas of the regenerating zebrafish heart across eight stages, including pre-injury, 6 hours post-amputation (hpa) of the ventricular apex, 12 hpa, 1 day post-amputation (dpa), 3 dpa, 7 dpa, 14 dpa, and 28 dpa. Using this comprehensive atlas, they characterized the dynamic changes in cell type composition throughout cardiac regeneration, including proliferating macrophages, pro-regenerative fibroblasts and regenerating cardiomyocytes. The study also identified <i>tpm4a</i> as a critical gene for cardiomyocyte re-differentiation during the regeneration process. Finally, the researchers created a ‘virtual regenerating heart’, encompassing both spatial information and time, which can be accessed here: https://db.cngb.org/stomics/zebrafish_VRH/</p><p><b>Original reference:</b> Li, L. et al. <i>Nat. Commun</i>. <b>16</b>, 3716 (2025)</p>\",\"PeriodicalId\":17936,\"journal\":{\"name\":\"Lab Animal\",\"volume\":\"260 1\",\"pages\":\"\"},\"PeriodicalIF\":5.9000,\"publicationDate\":\"2025-06-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lab Animal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1038/s41684-025-01569-6\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lab Animal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1038/s41684-025-01569-6","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
While adult mammals have an extremely limited capacity to repair lost or damaged heart tissue, many adult fish, including zebrafish, can successfully regenerate their hearts after injury. Uncovering the mechanisms of zebrafish heart regeneration could open new avenues to regenerate human hearts and prevent heart failure. In a new study, researchers combined the use of spatial transcriptomics (Stereo-seq) and single-cell RNA-sequencing (scRNA-seq) to generate a spatially-resolved molecular and cellular atlas of the regenerating zebrafish heart across eight stages, including pre-injury, 6 hours post-amputation (hpa) of the ventricular apex, 12 hpa, 1 day post-amputation (dpa), 3 dpa, 7 dpa, 14 dpa, and 28 dpa. Using this comprehensive atlas, they characterized the dynamic changes in cell type composition throughout cardiac regeneration, including proliferating macrophages, pro-regenerative fibroblasts and regenerating cardiomyocytes. The study also identified tpm4a as a critical gene for cardiomyocyte re-differentiation during the regeneration process. Finally, the researchers created a ‘virtual regenerating heart’, encompassing both spatial information and time, which can be accessed here: https://db.cngb.org/stomics/zebrafish_VRH/
Original reference: Li, L. et al. Nat. Commun. 16, 3716 (2025)
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LabAnimal is a Nature Research journal dedicated to in vivo science and technology that improves our basic understanding and use of model organisms of human health and disease. In addition to basic research, methods and technologies, LabAnimal also covers important news, business and regulatory matters that impact the development and application of model organisms for preclinical research.
LabAnimal's focus is on innovative in vivo methods, research and technology covering a wide range of model organisms. Our broad scope ensures that the work we publish reaches the widest possible audience. LabAnimal provides a rigorous and fair peer review of manuscripts, high standards for copyediting and production, and efficient publication.
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