{"title":"组织病理学检查在全球孢子丝菌引起的皮肤孢子菌病诊断中的作用：回顾性分析和再评价。","authors":"Liuyu Li, Jiajia Li, Zehu Liu, Xiujiao Xia","doi":"10.1111/myc.70072","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Due to the paucity of fungal elements in the tissue, the sensitivity of histopathological tests for the diagnosis of cutaneous sporotrichosis remains low, particularly for low-virulent Sporothrix species.</p><p><strong>Objectives: </strong>We retrospectively analysed and re-evaluated the role of histopathological examination in the diagnosis of cutaneous sporotrichosis caused by Sporothrix globosa.</p><p><strong>Methods: </strong>Retrospective analysis and pathological reassessment were conducted for 69 sporotrichosis cases caused by S. globosa between 2013 and 2024. Statistical analysis using prevalence ratios was conducted to characterise the clinicopathological and epidemiological aspects.</p><p><strong>Results: </strong>Histopathological examination revealed mixed inflammatory cell infiltration and granulomatous changes in all cases. Periodic acid-Schiff (PAS) staining was performed in 29 tissue specimens, with positivity observed in 4 cases (13.8%, 4/29). Following supplemental staining and re-evaluation of the slides, PAS staining revealed positivity in 50.7% (35/69) of cases, demonstrating yeast forms (91.4%, 32/35) with asteroid bodies (n = 2) and rare hyphae (n = 1).</p><p><strong>Conclusions: </strong>Currently, the role of histopathological examination in the diagnosis of sporotrichosis is limited. Our study showed that the frequency of detecting S. globosa based on histopathological examination is considerably high. Nevertheless, achieving high positive rates necessitates the collaborative efforts of experienced mycologists. The predominance of suppurative granulomas or neutrophils is related to the presence of the fungus in tissue sections from human patients.</p>","PeriodicalId":18797,"journal":{"name":"Mycoses","volume":"68 6","pages":"e70072"},"PeriodicalIF":3.1000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Role of Histopathological Examination in the Diagnosis of Cutaneous Sporotrichosis Caused by Sporothrix globosa: A Retrospective Analysis and Reassessment.\",\"authors\":\"Liuyu Li, Jiajia Li, Zehu Liu, Xiujiao Xia\",\"doi\":\"10.1111/myc.70072\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Due to the paucity of fungal elements in the tissue, the sensitivity of histopathological tests for the diagnosis of cutaneous sporotrichosis remains low, particularly for low-virulent Sporothrix species.</p><p><strong>Objectives: </strong>We retrospectively analysed and re-evaluated the role of histopathological examination in the diagnosis of cutaneous sporotrichosis caused by Sporothrix globosa.</p><p><strong>Methods: </strong>Retrospective analysis and pathological reassessment were conducted for 69 sporotrichosis cases caused by S. globosa between 2013 and 2024. Statistical analysis using prevalence ratios was conducted to characterise the clinicopathological and epidemiological aspects.</p><p><strong>Results: </strong>Histopathological examination revealed mixed inflammatory cell infiltration and granulomatous changes in all cases. Periodic acid-Schiff (PAS) staining was performed in 29 tissue specimens, with positivity observed in 4 cases (13.8%, 4/29). Following supplemental staining and re-evaluation of the slides, PAS staining revealed positivity in 50.7% (35/69) of cases, demonstrating yeast forms (91.4%, 32/35) with asteroid bodies (n = 2) and rare hyphae (n = 1).</p><p><strong>Conclusions: </strong>Currently, the role of histopathological examination in the diagnosis of sporotrichosis is limited. Our study showed that the frequency of detecting S. globosa based on histopathological examination is considerably high. Nevertheless, achieving high positive rates necessitates the collaborative efforts of experienced mycologists. The predominance of suppurative granulomas or neutrophils is related to the presence of the fungus in tissue sections from human patients.</p>\",\"PeriodicalId\":18797,\"journal\":{\"name\":\"Mycoses\",\"volume\":\"68 6\",\"pages\":\"e70072\"},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2025-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycoses\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/myc.70072\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycoses","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/myc.70072","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DERMATOLOGY","Score":null,"Total":0}
The Role of Histopathological Examination in the Diagnosis of Cutaneous Sporotrichosis Caused by Sporothrix globosa: A Retrospective Analysis and Reassessment.
Background: Due to the paucity of fungal elements in the tissue, the sensitivity of histopathological tests for the diagnosis of cutaneous sporotrichosis remains low, particularly for low-virulent Sporothrix species.
Objectives: We retrospectively analysed and re-evaluated the role of histopathological examination in the diagnosis of cutaneous sporotrichosis caused by Sporothrix globosa.
Methods: Retrospective analysis and pathological reassessment were conducted for 69 sporotrichosis cases caused by S. globosa between 2013 and 2024. Statistical analysis using prevalence ratios was conducted to characterise the clinicopathological and epidemiological aspects.
Results: Histopathological examination revealed mixed inflammatory cell infiltration and granulomatous changes in all cases. Periodic acid-Schiff (PAS) staining was performed in 29 tissue specimens, with positivity observed in 4 cases (13.8%, 4/29). Following supplemental staining and re-evaluation of the slides, PAS staining revealed positivity in 50.7% (35/69) of cases, demonstrating yeast forms (91.4%, 32/35) with asteroid bodies (n = 2) and rare hyphae (n = 1).
Conclusions: Currently, the role of histopathological examination in the diagnosis of sporotrichosis is limited. Our study showed that the frequency of detecting S. globosa based on histopathological examination is considerably high. Nevertheless, achieving high positive rates necessitates the collaborative efforts of experienced mycologists. The predominance of suppurative granulomas or neutrophils is related to the presence of the fungus in tissue sections from human patients.
期刊介绍：
The journal Mycoses provides an international forum for original papers in English on the pathogenesis, diagnosis, therapy, prophylaxis, and epidemiology of fungal infectious diseases in humans as well as on the biology of pathogenic fungi.
Medical mycology as part of medical microbiology is advancing rapidly. Effective therapeutic strategies are already available in chemotherapy and are being further developed. Their application requires reliable laboratory diagnostic techniques, which, in turn, result from mycological basic research. Opportunistic mycoses vary greatly in their clinical and pathological symptoms, because the underlying disease of a patient at risk decisively determines their symptomatology and progress. The journal Mycoses is therefore of interest to scientists in fundamental mycological research, mycological laboratory diagnosticians and clinicians interested in fungal infections.
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