Deluxeani Sureshkumar , Christy Jeyaseelan Emmanuel , Devika M. De Costa
{"title":"斯里兰卡葡萄藤炭疽病:病原体鉴定,流行病学见解,以及生物活性提取物的体外评价","authors":"Deluxeani Sureshkumar , Christy Jeyaseelan Emmanuel , Devika M. De Costa","doi":"10.1016/j.pmpp.2025.102771","DOIUrl":null,"url":null,"abstract":"<div><div>Anthracnose disease of grapevines, historically attributed to <em>Elsinoë ampelina</em>, has emerged as a significant concern in the Jaffna Peninsula, Sri Lanka. This study aimed to characterize the disease's etiology, epidemiology, and potential for biological control. Field assessments over two cultivation seasons (2022–2023) revealed a consistent pattern of disease progression, with peak incidence and severity occurring between 40 and 60 days post-pruning. Rainfall positively correlated with disease metrics, while wind speed showed a suppressive effect. Morphological and cultural characterization of 54 fungal isolates, coupled with multilocus phylogenetic analysis (ITS, actin, and histone H3), confirmed the presence of two distinct <em>Colletotrichum</em> morphotypes within the <em>C. gloeosporioides</em> species complex, potentially representing a novel lineage. Pathogenicity assays verified the virulence of representative isolates on grape leaves and fruits under both <em>in vitro</em> and in planta conditions. Furthermore, spore germination and lesion development were significantly inhibited by cell-free suspensions from <em>Pseudomonas fluorescens</em>, <em>Bacillus megaterium</em>, and ethanol extracts of <em>Azadirachta indica</em>, <em>Lantana camara</em>, <em>Vitex negundo</em>, <em>Stoechospermum marginatum</em>, and <em>Padina boryana</em>. These findings establish a members of new lineage of <em>C. gloeosporioides</em> complex as the primary causal agent of grape anthracnose in the region and highlight the promise of integrating biocontrol agents and botanical extracts into sustainable disease management programs.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"139 ","pages":"Article 102771"},"PeriodicalIF":2.8000,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anthracnose of grapevines in Sri Lanka: Pathogen identification, epidemiological insights, and in vitro evaluation of bioactive extracts\",\"authors\":\"Deluxeani Sureshkumar , Christy Jeyaseelan Emmanuel , Devika M. De Costa\",\"doi\":\"10.1016/j.pmpp.2025.102771\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Anthracnose disease of grapevines, historically attributed to <em>Elsinoë ampelina</em>, has emerged as a significant concern in the Jaffna Peninsula, Sri Lanka. This study aimed to characterize the disease's etiology, epidemiology, and potential for biological control. Field assessments over two cultivation seasons (2022–2023) revealed a consistent pattern of disease progression, with peak incidence and severity occurring between 40 and 60 days post-pruning. Rainfall positively correlated with disease metrics, while wind speed showed a suppressive effect. Morphological and cultural characterization of 54 fungal isolates, coupled with multilocus phylogenetic analysis (ITS, actin, and histone H3), confirmed the presence of two distinct <em>Colletotrichum</em> morphotypes within the <em>C. gloeosporioides</em> species complex, potentially representing a novel lineage. Pathogenicity assays verified the virulence of representative isolates on grape leaves and fruits under both <em>in vitro</em> and in planta conditions. Furthermore, spore germination and lesion development were significantly inhibited by cell-free suspensions from <em>Pseudomonas fluorescens</em>, <em>Bacillus megaterium</em>, and ethanol extracts of <em>Azadirachta indica</em>, <em>Lantana camara</em>, <em>Vitex negundo</em>, <em>Stoechospermum marginatum</em>, and <em>Padina boryana</em>. These findings establish a members of new lineage of <em>C. gloeosporioides</em> complex as the primary causal agent of grape anthracnose in the region and highlight the promise of integrating biocontrol agents and botanical extracts into sustainable disease management programs.</div></div>\",\"PeriodicalId\":20046,\"journal\":{\"name\":\"Physiological and Molecular Plant Pathology\",\"volume\":\"139 \",\"pages\":\"Article 102771\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-05-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physiological and Molecular Plant Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0885576525002103\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiological and Molecular Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0885576525002103","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Anthracnose of grapevines in Sri Lanka: Pathogen identification, epidemiological insights, and in vitro evaluation of bioactive extracts
Anthracnose disease of grapevines, historically attributed to Elsinoë ampelina, has emerged as a significant concern in the Jaffna Peninsula, Sri Lanka. This study aimed to characterize the disease's etiology, epidemiology, and potential for biological control. Field assessments over two cultivation seasons (2022–2023) revealed a consistent pattern of disease progression, with peak incidence and severity occurring between 40 and 60 days post-pruning. Rainfall positively correlated with disease metrics, while wind speed showed a suppressive effect. Morphological and cultural characterization of 54 fungal isolates, coupled with multilocus phylogenetic analysis (ITS, actin, and histone H3), confirmed the presence of two distinct Colletotrichum morphotypes within the C. gloeosporioides species complex, potentially representing a novel lineage. Pathogenicity assays verified the virulence of representative isolates on grape leaves and fruits under both in vitro and in planta conditions. Furthermore, spore germination and lesion development were significantly inhibited by cell-free suspensions from Pseudomonas fluorescens, Bacillus megaterium, and ethanol extracts of Azadirachta indica, Lantana camara, Vitex negundo, Stoechospermum marginatum, and Padina boryana. These findings establish a members of new lineage of C. gloeosporioides complex as the primary causal agent of grape anthracnose in the region and highlight the promise of integrating biocontrol agents and botanical extracts into sustainable disease management programs.
期刊介绍:
Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions.
Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.