{"title":"用于液体活检的下一代碳纳米管电化学传感器","authors":"Samuel Rantataro, Eero Gustafsson, Ilkka Varjos","doi":"10.1016/j.elecom.2025.107967","DOIUrl":null,"url":null,"abstract":"<div><div>The detection and quantification of circulating tumor DNA (ctDNA) from blood samples offers a non-invasive approach for cancer diagnostics and treatment monitoring. Although high- sensitivity and specificity assays are available based on polymerase chain reaction and next- generation sequencing, cost of these assays remains very high and results are typically achieved after days from sampling. Contrasting that, we report the development of a highly-sensitive electrochemical sensor assay, which is based on pristine carbon nanotube (CNT) electrodes functionalized with casein blocking layer and sequence-specific probes for ctDNA. The device demonstrated a 0.9 pM limit of detection towards ctDNA-related oligonucleotide sequences in undiluted human plasma, without applying DNA amplification strategy. Additionally, the assay was specific towards the target DNA and did not generate any distinguishable signal when incubated with wildtype sequence at high-concentration (500 pM). Contrasting to many other electrochemical sensor materials and assays that have previously demonstrated high sensitivity, our CNT-based assay provides results in less than an hour with a simple two-step assay. Additionally, the SWCNT sensors are manufactured at mass manufacturing scale, making this platform suitable for point-of-care applications.</div></div>","PeriodicalId":304,"journal":{"name":"Electrochemistry Communications","volume":"177 ","pages":"Article 107967"},"PeriodicalIF":4.2000,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Next-generation carbon nanotube electrochemical sensors for liquid biopsy applications\",\"authors\":\"Samuel Rantataro, Eero Gustafsson, Ilkka Varjos\",\"doi\":\"10.1016/j.elecom.2025.107967\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The detection and quantification of circulating tumor DNA (ctDNA) from blood samples offers a non-invasive approach for cancer diagnostics and treatment monitoring. Although high- sensitivity and specificity assays are available based on polymerase chain reaction and next- generation sequencing, cost of these assays remains very high and results are typically achieved after days from sampling. Contrasting that, we report the development of a highly-sensitive electrochemical sensor assay, which is based on pristine carbon nanotube (CNT) electrodes functionalized with casein blocking layer and sequence-specific probes for ctDNA. The device demonstrated a 0.9 pM limit of detection towards ctDNA-related oligonucleotide sequences in undiluted human plasma, without applying DNA amplification strategy. Additionally, the assay was specific towards the target DNA and did not generate any distinguishable signal when incubated with wildtype sequence at high-concentration (500 pM). Contrasting to many other electrochemical sensor materials and assays that have previously demonstrated high sensitivity, our CNT-based assay provides results in less than an hour with a simple two-step assay. Additionally, the SWCNT sensors are manufactured at mass manufacturing scale, making this platform suitable for point-of-care applications.</div></div>\",\"PeriodicalId\":304,\"journal\":{\"name\":\"Electrochemistry Communications\",\"volume\":\"177 \",\"pages\":\"Article 107967\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-05-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Electrochemistry Communications\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1388248125001067\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ELECTROCHEMISTRY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electrochemistry Communications","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1388248125001067","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ELECTROCHEMISTRY","Score":null,"Total":0}
Next-generation carbon nanotube electrochemical sensors for liquid biopsy applications
The detection and quantification of circulating tumor DNA (ctDNA) from blood samples offers a non-invasive approach for cancer diagnostics and treatment monitoring. Although high- sensitivity and specificity assays are available based on polymerase chain reaction and next- generation sequencing, cost of these assays remains very high and results are typically achieved after days from sampling. Contrasting that, we report the development of a highly-sensitive electrochemical sensor assay, which is based on pristine carbon nanotube (CNT) electrodes functionalized with casein blocking layer and sequence-specific probes for ctDNA. The device demonstrated a 0.9 pM limit of detection towards ctDNA-related oligonucleotide sequences in undiluted human plasma, without applying DNA amplification strategy. Additionally, the assay was specific towards the target DNA and did not generate any distinguishable signal when incubated with wildtype sequence at high-concentration (500 pM). Contrasting to many other electrochemical sensor materials and assays that have previously demonstrated high sensitivity, our CNT-based assay provides results in less than an hour with a simple two-step assay. Additionally, the SWCNT sensors are manufactured at mass manufacturing scale, making this platform suitable for point-of-care applications.
期刊介绍:
Electrochemistry Communications is an open access journal providing fast dissemination of short communications, full communications and mini reviews covering the whole field of electrochemistry which merit urgent publication. Short communications are limited to a maximum of 20,000 characters (including spaces) while full communications and mini reviews are limited to 25,000 characters (including spaces). Supplementary information is permitted for full communications and mini reviews but not for short communications. We aim to be the fastest journal in electrochemistry for these types of papers.