Viktoria Weinberger, Barbara Darnhofer, Himadri B. Thapa, Polona Mertelj, Régis Stentz, Emily Jones, Gerlinde Grabmann, Rokhsareh Mohammadzadeh, Tejus Shinde, Christina Karner, Jennifer Ober, Rokas Juodeikis, Dominique Pernitsch, Kerstin Hingerl, Tamara Zurabishvili, Christina Kumpitsch, Torben Kuehnast, Beate Rinner, Heimo Strohmaier, Dagmar Kolb, Kathryn Gotts, Thomas Weichhart, Thomas Köcher, Harald Köfeler, Simon R. Carding, Stefan Schild, Christine Moissl-Eichinger
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引用次数: 0
摘要
胃肠道细菌通过多种机制与宿主相互作用，包括产生细胞外囊泡（EVs）。然而，肠道古菌释放的ev的组成和潜在作用尚不清楚。在这里，我们研究了四株人类肠道来源的产甲烷古细菌产生的ev: Methanobrevibacter smithii ALI， M. smithii graz2， M. ninteini和Methanosphaera stadtmanae。这些电动汽车的尺寸（~130 nm）和形态与细菌电动汽车相当。蛋白质组学和代谢组学分析显示，古细菌EVs富含黏附素或黏附素样蛋白、游离谷氨酸和天冬氨酸，以及胆碱甘油磷酸。在体外，巨噬细胞摄取古细菌EVs，并在免疫和上皮细胞系中引起物种特异性反应，包括趋化因子如CXCL9、CXCL11和CX3CL1的产生。大肠杆菌产生的ev强烈诱导上皮细胞的促炎细胞因子IL-8。未来的工作应该研究古细菌ev是否在古细菌与其他肠道微生物和宿主的相互作用中发挥作用。
Proteomic and metabolomic profiling of extracellular vesicles produced by human gut archaea
Gastrointestinal bacteria interact with the host and each other through various mechanisms, including the production of extracellular vesicles (EVs). However, the composition and potential roles of EVs released by gut archaea are poorly understood. Here, we study EVs produced by four strains of human gut-derived methanogenic archaea: Methanobrevibacter smithii ALI, M. smithii GRAZ-2, M. intestini, and Methanosphaera stadtmanae. The size (~130 nm) and morphology of these EVs are comparable to those of bacterial EVs. Proteomic and metabolomic analyses reveal that the archaeal EVs are enriched in putative adhesins or adhesin-like proteins, free glutamic and aspartic acid, and choline glycerophosphate. The archaeal EVs are taken up by macrophages in vitro and elicit species-specific responses in immune and epithelial cell lines, including production of chemokines such as CXCL9, CXCL11, and CX3CL1. The EVs produced by M. intestini strongly induce pro-inflammatory cytokine IL-8 in epithelial cells. Future work should examine whether archaeal EVs play roles in the interactions of archaea with other gut microbes and with the host.
期刊介绍：
Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
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