{"title":"[基于化学生物学和生物物理学的单分子酶活性分析(SEAP)平台的建立]。","authors":"Toru Komatsu","doi":"10.1248/yakushi.24-00186-1","DOIUrl":null,"url":null,"abstract":"<p><p>In this study we have developed a method of profiling multiple \"single-molecules\" of enzymes in biological samples, by studying their activities as a form of single-molecule enzyme activity assay. The original method for single-molecule enzyme assay in microfabricated chamber devices was reported many years ago, but we for the first time report the application of this concept to identifying each enzyme in the chamber by simultaneously measuring activities against multiple substrates. Based on this idea, we developed the protein profiling technique to globally detect and \"count\" different sets of enzymes in biological samples containing various characterized and uncharacterized enzymes. We expect that the methodology will open up the application of single-molecule enzyme assay to discovering and using novel biomarker proteins.</p>","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 6","pages":"517-521"},"PeriodicalIF":0.2000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Establishment of Single-molecule Enzyme Activity Profiling (SEAP) Platform by Hybrid Technologies of Chemical Biology and Biophysics].\",\"authors\":\"Toru Komatsu\",\"doi\":\"10.1248/yakushi.24-00186-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In this study we have developed a method of profiling multiple \\\"single-molecules\\\" of enzymes in biological samples, by studying their activities as a form of single-molecule enzyme activity assay. The original method for single-molecule enzyme assay in microfabricated chamber devices was reported many years ago, but we for the first time report the application of this concept to identifying each enzyme in the chamber by simultaneously measuring activities against multiple substrates. Based on this idea, we developed the protein profiling technique to globally detect and \\\"count\\\" different sets of enzymes in biological samples containing various characterized and uncharacterized enzymes. We expect that the methodology will open up the application of single-molecule enzyme assay to discovering and using novel biomarker proteins.</p>\",\"PeriodicalId\":23810,\"journal\":{\"name\":\"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan\",\"volume\":\"145 6\",\"pages\":\"517-521\"},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1248/yakushi.24-00186-1\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1248/yakushi.24-00186-1","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
[Establishment of Single-molecule Enzyme Activity Profiling (SEAP) Platform by Hybrid Technologies of Chemical Biology and Biophysics].
In this study we have developed a method of profiling multiple "single-molecules" of enzymes in biological samples, by studying their activities as a form of single-molecule enzyme activity assay. The original method for single-molecule enzyme assay in microfabricated chamber devices was reported many years ago, but we for the first time report the application of this concept to identifying each enzyme in the chamber by simultaneously measuring activities against multiple substrates. Based on this idea, we developed the protein profiling technique to globally detect and "count" different sets of enzymes in biological samples containing various characterized and uncharacterized enzymes. We expect that the methodology will open up the application of single-molecule enzyme assay to discovering and using novel biomarker proteins.