{"title":"牛肠锥虫的首次分子检测及流行。","authors":"Omer Faruk Sahin, Ufuk Erol, Husnu Furkan Sakar, Kursat Altay","doi":"10.1007/s11686-025-01059-2","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Trypanosoma theileri is an opportunistic parasite that has worldwide distribution. This parasite has been detected in various hosts including cattle. Normally T. theileri can cause mild infection but it may lead to disease among animals in case of mixed infection or immunosuppression. In the present study, it was aimed to investigate the presence, prevalence, and to detect genotypes of T. theileri in cattle from different provinces in Türkiye using molecular techniques.</p><p><strong>Methods: </strong>In this study, 517 cattle blood collected from different provinces (Giresun, Samsun, Tokat, Sivas, Çorum, Trabzon, and Kastamonu) of Türkiye. Genomic DNA was extracted from blood samples using a commercial kit. The obtained gDNAs were screened for the presence of T. theileri using PCR. Partial parts of the CATL gene of randomly selected four positive samples were sequenced to determine the phylogenetic position of T. theileri Türkiye isolates.</p><p><strong>Results: </strong>PCR results showed that 18 out of 517 samples (3.48%) were positive in terms of T. theileri. The highest prevalence of T. theileri was observed in animals over 3 years of age (3.98%), followed by animals in the 1-3-year age group (3.16%). The prevalence of T. theileri was higher in female animals (3.62%) than in male animals (2.91%). The phylogenetic analyses of positive samples showed that T. theileri Türkiye isolates were clustered with the Tth IIB genotype of the T. theileri TthII lineage.</p><p><strong>Conclusion: </strong>In conclusion, the presence and prevalence of T. theileri in cattle were determined by molecular analyses for the first time in Türkiye. Moreover, Tth IIB genotype was also reported in Türkiye with this work. Although T. theileri is not thought to cause serious clinical symptoms in hosts, it should not be overlooked that it can cause significant economic losses in mixed infections with different pathogens.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":"122"},"PeriodicalIF":1.2000,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130095/pdf/","citationCount":"0","resultStr":"{\"title\":\"The First Molecular Detection of the Presence and Prevalence of Trypanosoma theileri in Cattle from Türkiye.\",\"authors\":\"Omer Faruk Sahin, Ufuk Erol, Husnu Furkan Sakar, Kursat Altay\",\"doi\":\"10.1007/s11686-025-01059-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Trypanosoma theileri is an opportunistic parasite that has worldwide distribution. This parasite has been detected in various hosts including cattle. Normally T. theileri can cause mild infection but it may lead to disease among animals in case of mixed infection or immunosuppression. In the present study, it was aimed to investigate the presence, prevalence, and to detect genotypes of T. theileri in cattle from different provinces in Türkiye using molecular techniques.</p><p><strong>Methods: </strong>In this study, 517 cattle blood collected from different provinces (Giresun, Samsun, Tokat, Sivas, Çorum, Trabzon, and Kastamonu) of Türkiye. Genomic DNA was extracted from blood samples using a commercial kit. The obtained gDNAs were screened for the presence of T. theileri using PCR. Partial parts of the CATL gene of randomly selected four positive samples were sequenced to determine the phylogenetic position of T. theileri Türkiye isolates.</p><p><strong>Results: </strong>PCR results showed that 18 out of 517 samples (3.48%) were positive in terms of T. theileri. The highest prevalence of T. theileri was observed in animals over 3 years of age (3.98%), followed by animals in the 1-3-year age group (3.16%). The prevalence of T. theileri was higher in female animals (3.62%) than in male animals (2.91%). The phylogenetic analyses of positive samples showed that T. theileri Türkiye isolates were clustered with the Tth IIB genotype of the T. theileri TthII lineage.</p><p><strong>Conclusion: </strong>In conclusion, the presence and prevalence of T. theileri in cattle were determined by molecular analyses for the first time in Türkiye. Moreover, Tth IIB genotype was also reported in Türkiye with this work. Although T. theileri is not thought to cause serious clinical symptoms in hosts, it should not be overlooked that it can cause significant economic losses in mixed infections with different pathogens.</p>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"70 3\",\"pages\":\"122\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2025-06-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130095/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s11686-025-01059-2\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s11686-025-01059-2","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
The First Molecular Detection of the Presence and Prevalence of Trypanosoma theileri in Cattle from Türkiye.
Purpose: Trypanosoma theileri is an opportunistic parasite that has worldwide distribution. This parasite has been detected in various hosts including cattle. Normally T. theileri can cause mild infection but it may lead to disease among animals in case of mixed infection or immunosuppression. In the present study, it was aimed to investigate the presence, prevalence, and to detect genotypes of T. theileri in cattle from different provinces in Türkiye using molecular techniques.
Methods: In this study, 517 cattle blood collected from different provinces (Giresun, Samsun, Tokat, Sivas, Çorum, Trabzon, and Kastamonu) of Türkiye. Genomic DNA was extracted from blood samples using a commercial kit. The obtained gDNAs were screened for the presence of T. theileri using PCR. Partial parts of the CATL gene of randomly selected four positive samples were sequenced to determine the phylogenetic position of T. theileri Türkiye isolates.
Results: PCR results showed that 18 out of 517 samples (3.48%) were positive in terms of T. theileri. The highest prevalence of T. theileri was observed in animals over 3 years of age (3.98%), followed by animals in the 1-3-year age group (3.16%). The prevalence of T. theileri was higher in female animals (3.62%) than in male animals (2.91%). The phylogenetic analyses of positive samples showed that T. theileri Türkiye isolates were clustered with the Tth IIB genotype of the T. theileri TthII lineage.
Conclusion: In conclusion, the presence and prevalence of T. theileri in cattle were determined by molecular analyses for the first time in Türkiye. Moreover, Tth IIB genotype was also reported in Türkiye with this work. Although T. theileri is not thought to cause serious clinical symptoms in hosts, it should not be overlooked that it can cause significant economic losses in mixed infections with different pathogens.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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