Zeinab R Hassan, Eman M Mahmoud, Yasmeen M Shaaban, Shiamaa Zakaria Elshora, Doaa E A Salama, Ranya M Abdelgalil, Mona T Koullah, Nora Seliem, Marwa H Sedira, Shimaa A Mohamed, Mai Ali Mohammad Etewa, Sara Nagdy Mahmoud Mousa, Ahmed Shaban Abdelmonsef Abdelmaksoud, Shimaa Attia Atta, Marwa Zakaria, Shimaa R Emam, M Hassan, Galal Khamis
{"title":"蓝色LED照射和未照射葫芦巴籽提取物对小鼠旋毛虫病的改善作用。","authors":"Zeinab R Hassan, Eman M Mahmoud, Yasmeen M Shaaban, Shiamaa Zakaria Elshora, Doaa E A Salama, Ranya M Abdelgalil, Mona T Koullah, Nora Seliem, Marwa H Sedira, Shimaa A Mohamed, Mai Ali Mohammad Etewa, Sara Nagdy Mahmoud Mousa, Ahmed Shaban Abdelmonsef Abdelmaksoud, Shimaa Attia Atta, Marwa Zakaria, Shimaa R Emam, M Hassan, Galal Khamis","doi":"10.1007/s11686-025-01045-8","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Trichinosis is a severe parasitic disease with a wide distribution and potential to affect humans. Available chemotherapeutic agents exhibit limited efficacy and are associated with numerous side effects. This study evaluated the ameliorative effects of blue LED irradiated and non-irradiated fenugreek seed extracts on experimental trichinosis.</p><p><strong>Methods: </strong>Eighty-four mice were divided into seven groups; each further subdivided into intestinal and muscular phases (six mice per subgroup): non-infected non-treated controls, infected non-treated controls, infected albendazole-treated, infected non-irradiated fenugreek extract-treated, infected blue LED irradiated fenugreek extract-treated, infected non-irradiated fenugreek and albendazole-treated, and infected blue LED irradiated fenugreek and albendazole-treated. Mice were sacrificed on the 7th-day post-infection for the intestinal phase and the 40th day for the muscular phase. Small intestine, muscle tissues, and serum samples were collected to assess parasitic load, histopathological changes, TGF-β immunohistochemical expression, serum IFN-γ levels, oxidative stress markers (MDA, nitrate/SOD, and catalase), and BAX gene expression as an apoptotic marker. Metabolomic profiling of extract was pursued to spot differential expression of metabolites.</p><p><strong>Results: </strong>Study outcomes demonstrated that blue LED irradiated and non-irradiated fenugreek seed extracts combined with albendazole exhibited superior efficacy in reducing adult and larval burdens, improving pathological changes, decreasing IFN-γ levels, mitigating oxidative stress (reduced MDA and nitrate along with elevated SOD and catalase), and downregulating BAX expression. The observed metabolic differences were primarily driven by the upregulation of steroids, downregulation of most alkaloids, and dysregulation various flavonoids in the irradiated extract.</p><p><strong>Conclusion: </strong>Blue LED irradiated and non-irradiated fenugreek seed extracts can enhance albendazole's activity against trichinosis.</p>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":"119"},"PeriodicalIF":1.5000,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130083/pdf/","citationCount":"0","resultStr":"{\"title\":\"Ameliorative Effects of Blue LED Irradiated and Non-Irradiated Fenugreek Seed Extracts on Murine Trichinosis.\",\"authors\":\"Zeinab R Hassan, Eman M Mahmoud, Yasmeen M Shaaban, Shiamaa Zakaria Elshora, Doaa E A Salama, Ranya M Abdelgalil, Mona T Koullah, Nora Seliem, Marwa H Sedira, Shimaa A Mohamed, Mai Ali Mohammad Etewa, Sara Nagdy Mahmoud Mousa, Ahmed Shaban Abdelmonsef Abdelmaksoud, Shimaa Attia Atta, Marwa Zakaria, Shimaa R Emam, M Hassan, Galal Khamis\",\"doi\":\"10.1007/s11686-025-01045-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Trichinosis is a severe parasitic disease with a wide distribution and potential to affect humans. Available chemotherapeutic agents exhibit limited efficacy and are associated with numerous side effects. This study evaluated the ameliorative effects of blue LED irradiated and non-irradiated fenugreek seed extracts on experimental trichinosis.</p><p><strong>Methods: </strong>Eighty-four mice were divided into seven groups; each further subdivided into intestinal and muscular phases (six mice per subgroup): non-infected non-treated controls, infected non-treated controls, infected albendazole-treated, infected non-irradiated fenugreek extract-treated, infected blue LED irradiated fenugreek extract-treated, infected non-irradiated fenugreek and albendazole-treated, and infected blue LED irradiated fenugreek and albendazole-treated. Mice were sacrificed on the 7th-day post-infection for the intestinal phase and the 40th day for the muscular phase. Small intestine, muscle tissues, and serum samples were collected to assess parasitic load, histopathological changes, TGF-β immunohistochemical expression, serum IFN-γ levels, oxidative stress markers (MDA, nitrate/SOD, and catalase), and BAX gene expression as an apoptotic marker. Metabolomic profiling of extract was pursued to spot differential expression of metabolites.</p><p><strong>Results: </strong>Study outcomes demonstrated that blue LED irradiated and non-irradiated fenugreek seed extracts combined with albendazole exhibited superior efficacy in reducing adult and larval burdens, improving pathological changes, decreasing IFN-γ levels, mitigating oxidative stress (reduced MDA and nitrate along with elevated SOD and catalase), and downregulating BAX expression. The observed metabolic differences were primarily driven by the upregulation of steroids, downregulation of most alkaloids, and dysregulation various flavonoids in the irradiated extract.</p><p><strong>Conclusion: </strong>Blue LED irradiated and non-irradiated fenugreek seed extracts can enhance albendazole's activity against trichinosis.</p>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"70 3\",\"pages\":\"119\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-06-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130083/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s11686-025-01045-8\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s11686-025-01045-8","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Ameliorative Effects of Blue LED Irradiated and Non-Irradiated Fenugreek Seed Extracts on Murine Trichinosis.
Background: Trichinosis is a severe parasitic disease with a wide distribution and potential to affect humans. Available chemotherapeutic agents exhibit limited efficacy and are associated with numerous side effects. This study evaluated the ameliorative effects of blue LED irradiated and non-irradiated fenugreek seed extracts on experimental trichinosis.
Methods: Eighty-four mice were divided into seven groups; each further subdivided into intestinal and muscular phases (six mice per subgroup): non-infected non-treated controls, infected non-treated controls, infected albendazole-treated, infected non-irradiated fenugreek extract-treated, infected blue LED irradiated fenugreek extract-treated, infected non-irradiated fenugreek and albendazole-treated, and infected blue LED irradiated fenugreek and albendazole-treated. Mice were sacrificed on the 7th-day post-infection for the intestinal phase and the 40th day for the muscular phase. Small intestine, muscle tissues, and serum samples were collected to assess parasitic load, histopathological changes, TGF-β immunohistochemical expression, serum IFN-γ levels, oxidative stress markers (MDA, nitrate/SOD, and catalase), and BAX gene expression as an apoptotic marker. Metabolomic profiling of extract was pursued to spot differential expression of metabolites.
Results: Study outcomes demonstrated that blue LED irradiated and non-irradiated fenugreek seed extracts combined with albendazole exhibited superior efficacy in reducing adult and larval burdens, improving pathological changes, decreasing IFN-γ levels, mitigating oxidative stress (reduced MDA and nitrate along with elevated SOD and catalase), and downregulating BAX expression. The observed metabolic differences were primarily driven by the upregulation of steroids, downregulation of most alkaloids, and dysregulation various flavonoids in the irradiated extract.
Conclusion: Blue LED irradiated and non-irradiated fenugreek seed extracts can enhance albendazole's activity against trichinosis.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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