α-生育酚(维生素E)对全水合二酰磷脂酰胆碱膜相行为的影响。

IF 2.8 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Chi Long Chan , Divya Malia , Miguel Paez-Perez , Laurent Sagalowicz , Olivier Schafer , Robert V. Law , Nicholas J. Brooks , John M. Seddon
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引用次数: 0

摘要

用同步小角x射线衍射研究了α-生育酚(α-toc)与不饱和磷脂二油基磷脂酰胆碱(DOPC)的完全水合混合物的溶变相行为。α-toc含量的增加使DOPC流体层状Lα相的层间距逐渐膨胀,并诱导其向逆六方HII相转变。低分辨率电子密度谱显示,这种增加主要是由于双层厚度的增加,而水层厚度变化不大。在30 ~ 50mol% α-toc范围内,观察到额外的弱低角峰,其特征比与空间群Im3m / Pn3m存在膨胀的逆双连续立方相一致。这项研究在生物领域和工业产品开发中都有应用。我们发现α-toc在DOPC膜中的作用与胆固醇的作用有一些相似之处,即稳定了在细胞分裂、膜运输和内吞作用中起关键作用的逆曲率结构。在工业应用方面,倒六边形(HII)和膨胀双连续立方相的稳定为开发新的输送系统提供了机会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effect of α-tocopherol (vitamin E) on the phase behaviour of fully-hydrated dioleoyl phosphatidylcholine membranes
We report on the lyotropic phase behaviour of fully-hydrated mixtures of α-tocopherol (α-toc) with the unsaturated phospholipid dioleoyl phosphatidylcholine (DOPC), as studied by synchrotron small-angle x-ray diffraction. Increasing amounts of α-toc progressively swell the layer spacing of the fluid lamellar Lα phase of DOPC, and then induce a transition to an inverse hexagonal HII phase. Low-resolution electron density profiles show that this increase is largely due to an increased thickness of the bilayer, with little change in the water layer thickness. In the range 30 – 50 mol% α-toc, additional weak low-angle peaks were observed, whose characteristic ratios are in agreement with the presence of swollen inverse bicontinuous cubic phases of spacegroups Im3m / Pn3m. This research has applications both in the biological field and for industrial product development. We show that the effect of α-toc addition in DOPC membranes has some similarities to that of cholesterol by stabilizing inverse curvature structures, which play crucial roles in cell division, membrane trafficking and endocytosis. Concerning industrial applications, the stabilization of inverted hexagonal (HII) and swollen bicontinuous cubic phases offers the opportunity to develop new delivery systems.
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来源期刊
Chemistry and Physics of Lipids
Chemistry and Physics of Lipids 生物-生化与分子生物学
CiteScore
7.60
自引率
2.90%
发文量
50
审稿时长
40 days
期刊介绍: Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.
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