揭示翻译后修饰的复杂性:定量组蛋白蛋白质谱的进展。

IF 6.6 2区 化学 Q1 SPECTROSCOPY
Karl F Poncha, Alyssa T Paparella, Nicolas L Young
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引用次数: 0

摘要

组蛋白及其翻译后修饰是染色质结构和功能的核心。这些修饰通常发生在组合中,产生多种组蛋白蛋白形式,有助于染色质结构的动态调节。基于质谱的蛋白质组学的进步,特别是自上而下和中向下的方法,大大提高了我们表征这些蛋白质形态和阐明PTM串扰的能力。本文对组蛋白PTM的添加、识别和去除的表观遗传机制进行了分析,强调了组蛋白变异和组合PTM模式引入的复杂性。我们研究了传统的基于抗体的PTM分析方法的挑战和局限性,并强调了质谱技术在提供对组蛋白蛋白形态的全面和定量见解方面的优势。在实验设计、样品制备、色谱分离和数据分析方面的关键考虑因素概述了有效应用质谱法进行组蛋白蛋白形成研究。通过整合样品制备、仪器和数据处理方面的这些技术进步,通过PTM串扰实现了对染色质调控的更深入理解,为基于质谱的蛋白质组学率先发现具有蛋白质形态水平特异性的新型治疗策略铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unraveling Posttranslational Modification Complexity: Advances in Quantitative Histone Proteoform Mass Spectrometry.

Histone proteins and their posttranslational modifications are central to chromatin structure and function. These modifications often occur in combinations, generating a diverse array of histone proteoforms that contribute to the dynamic regulation of chromatin architecture. Advancements in mass spectrometry-based proteomics, particularly top-down and middle-down approaches, have significantly enhanced our ability to characterize these proteoforms and elucidate PTM crosstalk. This review provides an analysis of the epigenetic machinery involved in the addition, recognition, and removal of histone PTMs, emphasizing the complexity introduced by histone variants and combinatorial PTM patterns. We examine the challenges and limitations of traditional antibody-based methods for PTM analysis and highlight the advantages of mass spectrometry techniques in providing comprehensive and quantitative insights into histone proteoforms. Key considerations in experimental design, sample preparation, chromatographic separation, and data analysis are outlined for the effective application of mass spectrometry for histone proteoform studies. By integrating these technological advancements on the side of sample preparation, instrumentation, and data processing a deeper understanding of chromatin regulation through PTM crosstalk is achieved, paving the way for mass spectrometry-based proteomics to spearhead the discovery of novel therapeutic strategies with proteoform level specificity.

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来源期刊
Mass Spectrometry Reviews
Mass Spectrometry Reviews 物理-光谱学
CiteScore
16.30
自引率
3.00%
发文量
56
期刊介绍: The aim of the journal Mass Spectrometry Reviews is to publish well-written reviews in selected topics in the various sub-fields of mass spectrometry as a means to summarize the research that has been performed in that area, to focus attention of other researchers, to critically review the published material, and to stimulate further research in that area. The scope of the published reviews include, but are not limited to topics, such as theoretical treatments, instrumental design, ionization methods, analyzers, detectors, application to the qualitative and quantitative analysis of various compounds or elements, basic ion chemistry and structure studies, ion energetic studies, and studies on biomolecules, polymers, etc.
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