转移性黑色素瘤患者循环肿瘤细胞的微流控表征和分析

IF 2.6 3区 医学 Q2 CELL BIOLOGY
Matthew C. Mannino, Shuang G. Zhao, Benjamin K. Gibbs, Jennifer L. Schehr, Isabella G. Fernandez, Diego A. Eyzaguirre, Alyssa M. Hintz, Stephanie J. Davis, Manushi N. Vatani, Jacob C. Caceres, Alexander Birbrair, Joshua M. Lang, Vincent T. Ma
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引用次数: 0

摘要

循环肿瘤细胞(CTCs)可以提供非侵入性的洞察癌症患者对治疗的反应。它们在免疫检查点抑制剂(ICI)治疗的晚期黑色素瘤患者的疾病监测中的作用尚不清楚。人类白细胞抗原I类(HLA I)和程序性死亡配体-1 (PD-L1)的CTC蛋白表达可能有助于了解患者疾病在治疗过程中的演变。在我们的研究中,我们利用基于微流控排斥的样品制备(ESP)技术从晚期黑色素瘤患者中分离和表征ctc。收集、捕获黑色素瘤患者的CTC样本并进行染色。在接受ICI治疗的10例晚期黑色素瘤患者的16个样本队列中观察到2至35个ctc。单细胞蛋白表达数据由图像细胞术分析生成,用于计算HLA I和PD-L1的平均表达。使用我们的ESP捕获方法,我们成功地检测了黑色素瘤患者ctc的表型和数值异质性。我们的分析显示出足够的捕获灵敏度和有希望的预后和预测信息,正如我们在案例中所说明的那样。需要更大的临床样本量来确认该方法在预测晚期黑色素瘤患者临床结果方面的诊断敏感性和特异性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Microfluidic Characterization and Analysis of Circulating Tumor Cells From Patients With Metastatic Melanoma

Microfluidic Characterization and Analysis of Circulating Tumor Cells From Patients With Metastatic Melanoma

Circulating tumor cells (CTCs) can provide non-invasive insight into how a cancer patient responds to therapy. Their role in disease monitoring of advanced melanoma patients treated with immune checkpoint inhibitors (ICI) is unknown. CTC protein expression of human leukocyte antigen class-I (HLA I) and programmed death ligand-1 (PD-L1) may give insight into how a patient's disease evolves over the course of treatment. In our study, we utilize microfluidic Exclusion-based Sample Preparation (ESP) technology to isolate and characterize CTCs from patients with advanced-stage melanoma. CTC samples from melanoma patients are collected, captured, and stained. A range of 2 to 35 CTCs is observed in a cohort of 16 samples from 10 advanced-stage melanoma patients treated with ICI therapy. Single-cell protein expression data is generated from image cytometry analysis and used to calculate mean HLA I and PD-L1 expression. Using our ESP capture approach, we successfully detect phenotypic and numerical heterogeneity in CTCs from melanoma patients. Our assay shows sufficient capture sensitivity and promising prognostic and predictive information, as we illustrate in our case example. A greater clinical sample size will be necessary to confirm the diagnostic sensitivity and specificity of the assay in predicting clinical outcomes for patients with advanced-stage melanoma.

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来源期刊
Pigment Cell & Melanoma Research
Pigment Cell & Melanoma Research 医学-皮肤病学
CiteScore
8.90
自引率
2.30%
发文量
54
审稿时长
6-12 weeks
期刊介绍: Pigment Cell & Melanoma Researchpublishes manuscripts on all aspects of pigment cells including development, cell and molecular biology, genetics, diseases of pigment cells including melanoma. Papers that provide insights into the causes and progression of melanoma including the process of metastasis and invasion, proliferation, senescence, apoptosis or gene regulation are especially welcome, as are papers that use the melanocyte system to answer questions of general biological relevance. Papers that are purely descriptive or make only minor advances to our knowledge of pigment cells or melanoma in particular are not suitable for this journal. Keywords Pigment Cell & Melanoma Research, cell biology, melatonin, biochemistry, chemistry, comparative biology, dermatology, developmental biology, genetics, hormones, intracellular signalling, melanoma, molecular biology, ocular and extracutaneous melanin, pharmacology, photobiology, physics, pigmentary disorders
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