Matthew C. Mannino, Shuang G. Zhao, Benjamin K. Gibbs, Jennifer L. Schehr, Isabella G. Fernandez, Diego A. Eyzaguirre, Alyssa M. Hintz, Stephanie J. Davis, Manushi N. Vatani, Jacob C. Caceres, Alexander Birbrair, Joshua M. Lang, Vincent T. Ma
{"title":"转移性黑色素瘤患者循环肿瘤细胞的微流控表征和分析","authors":"Matthew C. Mannino, Shuang G. Zhao, Benjamin K. Gibbs, Jennifer L. Schehr, Isabella G. Fernandez, Diego A. Eyzaguirre, Alyssa M. Hintz, Stephanie J. Davis, Manushi N. Vatani, Jacob C. Caceres, Alexander Birbrair, Joshua M. Lang, Vincent T. Ma","doi":"10.1111/pcmr.70030","DOIUrl":null,"url":null,"abstract":"<p>Circulating tumor cells (CTCs) can provide non-invasive insight into how a cancer patient responds to therapy. Their role in disease monitoring of advanced melanoma patients treated with immune checkpoint inhibitors (ICI) is unknown. CTC protein expression of human leukocyte antigen class-I (HLA I) and programmed death ligand-1 (PD-L1) may give insight into how a patient's disease evolves over the course of treatment. In our study, we utilize microfluidic Exclusion-based Sample Preparation (ESP) technology to isolate and characterize CTCs from patients with advanced-stage melanoma. CTC samples from melanoma patients are collected, captured, and stained. A range of 2 to 35 CTCs is observed in a cohort of 16 samples from 10 advanced-stage melanoma patients treated with ICI therapy. Single-cell protein expression data is generated from image cytometry analysis and used to calculate mean HLA I and PD-L1 expression. Using our ESP capture approach, we successfully detect phenotypic and numerical heterogeneity in CTCs from melanoma patients. Our assay shows sufficient capture sensitivity and promising prognostic and predictive information, as we illustrate in our case example. A greater clinical sample size will be necessary to confirm the diagnostic sensitivity and specificity of the assay in predicting clinical outcomes for patients with advanced-stage melanoma.</p>","PeriodicalId":219,"journal":{"name":"Pigment Cell & Melanoma Research","volume":"38 4","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/pcmr.70030","citationCount":"0","resultStr":"{\"title\":\"Microfluidic Characterization and Analysis of Circulating Tumor Cells From Patients With Metastatic Melanoma\",\"authors\":\"Matthew C. Mannino, Shuang G. Zhao, Benjamin K. Gibbs, Jennifer L. Schehr, Isabella G. Fernandez, Diego A. Eyzaguirre, Alyssa M. Hintz, Stephanie J. Davis, Manushi N. Vatani, Jacob C. Caceres, Alexander Birbrair, Joshua M. Lang, Vincent T. 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Microfluidic Characterization and Analysis of Circulating Tumor Cells From Patients With Metastatic Melanoma
Circulating tumor cells (CTCs) can provide non-invasive insight into how a cancer patient responds to therapy. Their role in disease monitoring of advanced melanoma patients treated with immune checkpoint inhibitors (ICI) is unknown. CTC protein expression of human leukocyte antigen class-I (HLA I) and programmed death ligand-1 (PD-L1) may give insight into how a patient's disease evolves over the course of treatment. In our study, we utilize microfluidic Exclusion-based Sample Preparation (ESP) technology to isolate and characterize CTCs from patients with advanced-stage melanoma. CTC samples from melanoma patients are collected, captured, and stained. A range of 2 to 35 CTCs is observed in a cohort of 16 samples from 10 advanced-stage melanoma patients treated with ICI therapy. Single-cell protein expression data is generated from image cytometry analysis and used to calculate mean HLA I and PD-L1 expression. Using our ESP capture approach, we successfully detect phenotypic and numerical heterogeneity in CTCs from melanoma patients. Our assay shows sufficient capture sensitivity and promising prognostic and predictive information, as we illustrate in our case example. A greater clinical sample size will be necessary to confirm the diagnostic sensitivity and specificity of the assay in predicting clinical outcomes for patients with advanced-stage melanoma.
期刊介绍:
Pigment Cell & Melanoma Researchpublishes manuscripts on all aspects of pigment cells including development, cell and molecular biology, genetics, diseases of pigment cells including melanoma. Papers that provide insights into the causes and progression of melanoma including the process of metastasis and invasion, proliferation, senescence, apoptosis or gene regulation are especially welcome, as are papers that use the melanocyte system to answer questions of general biological relevance. Papers that are purely descriptive or make only minor advances to our knowledge of pigment cells or melanoma in particular are not suitable for this journal. Keywords
Pigment Cell & Melanoma Research, cell biology, melatonin, biochemistry, chemistry, comparative biology, dermatology, developmental biology, genetics, hormones, intracellular signalling, melanoma, molecular biology, ocular and extracutaneous melanin, pharmacology, photobiology, physics, pigmentary disorders