Mingzhuo Li, Xun Chen, JingJing Ye, Yudong Sun, Changhao Hou, Nailong Cao, Garth J. Thompson, Baojun Gu
{"title":"糖尿病性膀胱功能障碍大鼠脑活动的功能MRI分析","authors":"Mingzhuo Li, Xun Chen, JingJing Ye, Yudong Sun, Changhao Hou, Nailong Cao, Garth J. Thompson, Baojun Gu","doi":"10.1111/cns.70466","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aims</h3>\n \n <p>This study aimed to investigate brain activity using functional magnetic resonance imaging (fMRI) in rats with 12-week diabetic bladder dysfunction (DBD). Furthermore, as prior research has suggested that NLX-112, a 5-HT<sub>1A</sub> receptor agonist, may alleviate DBD, we sought to explore its effects on brain activity in DBD rats.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Male Sprague–Dawley rats were anesthetized with urethane and underwent cystometry alongside 9.4-Tesla fMRI evaluations. Resting-state fMRI was performed on empty bladders to compare baseline brain activity between groups. Gradient echo-planar imaging was utilized to assess brain activation during micturition relative to relaxation, and a group analysis was conducted.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>fMRI data from 12 diabetes mellitus (DM) rats and 12 normal control (NC) rats were analyzed. DM rats exhibited a significant reduction in the amplitude of low-frequency fluctuation (ALFF) in the basal forebrain and cerebral cortex, a measurement of activity in brain networks. During micturition, DM rats showed increased activation in the thalamus, primary motor cortex, periaqueductal gray (PAG) and other regions. NLX-112 administration did not significantly alter brain activity in DM rats.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>DBD rats exhibit heightened thalamic and PAG activity during micturition, potentially due to enlarged bladder capacity, with cortical activity serving as a compensatory mechanism. These findings highlight potential neural targets for DBD treatment.</p>\n </section>\n </div>","PeriodicalId":154,"journal":{"name":"CNS Neuroscience & Therapeutics","volume":"31 6","pages":""},"PeriodicalIF":5.0000,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cns.70466","citationCount":"0","resultStr":"{\"title\":\"Functional MRI Analysis of Brain Activity in Rats With Diabetic Bladder Dysfunction\",\"authors\":\"Mingzhuo Li, Xun Chen, JingJing Ye, Yudong Sun, Changhao Hou, Nailong Cao, Garth J. Thompson, Baojun Gu\",\"doi\":\"10.1111/cns.70466\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Aims</h3>\\n \\n <p>This study aimed to investigate brain activity using functional magnetic resonance imaging (fMRI) in rats with 12-week diabetic bladder dysfunction (DBD). Furthermore, as prior research has suggested that NLX-112, a 5-HT<sub>1A</sub> receptor agonist, may alleviate DBD, we sought to explore its effects on brain activity in DBD rats.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Male Sprague–Dawley rats were anesthetized with urethane and underwent cystometry alongside 9.4-Tesla fMRI evaluations. Resting-state fMRI was performed on empty bladders to compare baseline brain activity between groups. Gradient echo-planar imaging was utilized to assess brain activation during micturition relative to relaxation, and a group analysis was conducted.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>fMRI data from 12 diabetes mellitus (DM) rats and 12 normal control (NC) rats were analyzed. DM rats exhibited a significant reduction in the amplitude of low-frequency fluctuation (ALFF) in the basal forebrain and cerebral cortex, a measurement of activity in brain networks. 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Functional MRI Analysis of Brain Activity in Rats With Diabetic Bladder Dysfunction
Aims
This study aimed to investigate brain activity using functional magnetic resonance imaging (fMRI) in rats with 12-week diabetic bladder dysfunction (DBD). Furthermore, as prior research has suggested that NLX-112, a 5-HT1A receptor agonist, may alleviate DBD, we sought to explore its effects on brain activity in DBD rats.
Methods
Male Sprague–Dawley rats were anesthetized with urethane and underwent cystometry alongside 9.4-Tesla fMRI evaluations. Resting-state fMRI was performed on empty bladders to compare baseline brain activity between groups. Gradient echo-planar imaging was utilized to assess brain activation during micturition relative to relaxation, and a group analysis was conducted.
Results
fMRI data from 12 diabetes mellitus (DM) rats and 12 normal control (NC) rats were analyzed. DM rats exhibited a significant reduction in the amplitude of low-frequency fluctuation (ALFF) in the basal forebrain and cerebral cortex, a measurement of activity in brain networks. During micturition, DM rats showed increased activation in the thalamus, primary motor cortex, periaqueductal gray (PAG) and other regions. NLX-112 administration did not significantly alter brain activity in DM rats.
Conclusion
DBD rats exhibit heightened thalamic and PAG activity during micturition, potentially due to enlarged bladder capacity, with cortical activity serving as a compensatory mechanism. These findings highlight potential neural targets for DBD treatment.
期刊介绍：
CNS Neuroscience & Therapeutics provides a medium for rapid publication of original clinical, experimental, and translational research papers, timely reviews and reports of novel findings of therapeutic relevance to the central nervous system, as well as papers related to clinical pharmacology, drug development and novel methodologies for drug evaluation. The journal focuses on neurological and psychiatric diseases such as stroke, Parkinson’s disease, Alzheimer’s disease, depression, schizophrenia, epilepsy, and drug abuse.
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