天气和叶龄分别对山艾树叶层真菌群落结构的时间变化有影响

IF 2.9 3区 环境科学与生态学 Q2 ECOLOGY
Ecosphere Pub Date : 2025-06-03 DOI:10.1002/ecs2.70295
Jacob A. Heil, Allison Simler-Williamson, Miranda L. Striluk, Danielle Trawick, Rachel Capezza, Chadwick DeFehr, Aubrey Osorio, Bruce Finney, Kathryn G. Turner, Leonora S. Bittleston
{"title":"天气和叶龄分别对山艾树叶层真菌群落结构的时间变化有影响","authors":"Jacob A. Heil,&nbsp;Allison Simler-Williamson,&nbsp;Miranda L. Striluk,&nbsp;Danielle Trawick,&nbsp;Rachel Capezza,&nbsp;Chadwick DeFehr,&nbsp;Aubrey Osorio,&nbsp;Bruce Finney,&nbsp;Kathryn G. Turner,&nbsp;Leonora S. Bittleston","doi":"10.1002/ecs2.70295","DOIUrl":null,"url":null,"abstract":"<p>Microbial communities living on plant leaves can positively or negatively influence plant health and, by extension, can impact whole ecosystems. Most research into the leaf microbiome consists of snapshots, and little is known about how microbial communities change over time. Weather and host physiological characteristics change over time and are often collinear with other time-varying factors, such as substrate availability, making it difficult to separate the factors driving microbial community change. We leveraged repeated measures over the course of an entire year to isolate the relative importance of environmental, host physiological, and substrate age-related factors on the structure of leaf-associated fungal communities. We applied both culturing and sequencing approaches to investigate these communities, focusing on a foundational, widely distributed plant of conservation concern: basin big sagebrush (<i>Artemisia tridentata</i> subsp. <i>tridentata</i>). We found that changes in alpha diversity were independently affected by the age of leaves and the air temperature. Total fungal abundance and species richness were not positively correlated and responded differently, sometimes oppositely, to weather. With regard to beta diversity, communities were more similar to each other across similar leaf ages, air temperatures, leaf types, and δ<sup>13</sup>C stable isotope ratios. Nine different genera were differentially abundant with air temperature, δ<sup>13</sup>C, leaf type, and leaf age, and a set of 20 genera were continuously present across the year. Our findings highlight the necessity for longer term, repeated sampling to parse drivers of temporal change in leaf microbial communities.</p>","PeriodicalId":48930,"journal":{"name":"Ecosphere","volume":"16 6","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ecs2.70295","citationCount":"0","resultStr":"{\"title\":\"Weather and leaf age separately contribute to temporal shifts in phyllosphere fungal community structure in sagebrush\",\"authors\":\"Jacob A. Heil,&nbsp;Allison Simler-Williamson,&nbsp;Miranda L. Striluk,&nbsp;Danielle Trawick,&nbsp;Rachel Capezza,&nbsp;Chadwick DeFehr,&nbsp;Aubrey Osorio,&nbsp;Bruce Finney,&nbsp;Kathryn G. Turner,&nbsp;Leonora S. Bittleston\",\"doi\":\"10.1002/ecs2.70295\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Microbial communities living on plant leaves can positively or negatively influence plant health and, by extension, can impact whole ecosystems. Most research into the leaf microbiome consists of snapshots, and little is known about how microbial communities change over time. Weather and host physiological characteristics change over time and are often collinear with other time-varying factors, such as substrate availability, making it difficult to separate the factors driving microbial community change. We leveraged repeated measures over the course of an entire year to isolate the relative importance of environmental, host physiological, and substrate age-related factors on the structure of leaf-associated fungal communities. We applied both culturing and sequencing approaches to investigate these communities, focusing on a foundational, widely distributed plant of conservation concern: basin big sagebrush (<i>Artemisia tridentata</i> subsp. <i>tridentata</i>). We found that changes in alpha diversity were independently affected by the age of leaves and the air temperature. Total fungal abundance and species richness were not positively correlated and responded differently, sometimes oppositely, to weather. With regard to beta diversity, communities were more similar to each other across similar leaf ages, air temperatures, leaf types, and δ<sup>13</sup>C stable isotope ratios. Nine different genera were differentially abundant with air temperature, δ<sup>13</sup>C, leaf type, and leaf age, and a set of 20 genera were continuously present across the year. Our findings highlight the necessity for longer term, repeated sampling to parse drivers of temporal change in leaf microbial communities.</p>\",\"PeriodicalId\":48930,\"journal\":{\"name\":\"Ecosphere\",\"volume\":\"16 6\",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-06-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ecs2.70295\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ecosphere\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/ecs2.70295\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ecosphere","FirstCategoryId":"93","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ecs2.70295","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

生活在植物叶片上的微生物群落可以对植物健康产生积极或消极的影响,进而影响整个生态系统。大多数对叶片微生物组的研究都是由快照组成的,对微生物群落如何随时间变化知之甚少。天气和宿主生理特征随时间而变化,并且通常与其他时变因素(如底物可利用性)共线性,因此很难分离驱动微生物群落变化的因素。我们利用一整年的重复测量来分离环境、寄主生理和底物年龄相关因素对叶片相关真菌群落结构的相对重要性。我们采用培养和测序两种方法对这些群落进行了调查,重点研究了一种基础的、广泛分布的保护植物:盆地大艾草(Artemisia tridentata subsp.)。tridentata)。我们发现,α多样性的变化独立受叶片年龄和气温的影响。真菌总丰度和物种丰富度对气候的响应不同,有时相反。在β多样性方面,相似叶龄、气温、叶型和δ13C稳定同位素比值的群落之间具有更大的相似性。9个属在气温、δ13C、叶型、叶龄等方面存在差异丰度,有20个属全年连续存在。我们的研究结果强调了长期重复采样的必要性,以分析叶片微生物群落时间变化的驱动因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Weather and leaf age separately contribute to temporal shifts in phyllosphere fungal community structure in sagebrush

Weather and leaf age separately contribute to temporal shifts in phyllosphere fungal community structure in sagebrush

Microbial communities living on plant leaves can positively or negatively influence plant health and, by extension, can impact whole ecosystems. Most research into the leaf microbiome consists of snapshots, and little is known about how microbial communities change over time. Weather and host physiological characteristics change over time and are often collinear with other time-varying factors, such as substrate availability, making it difficult to separate the factors driving microbial community change. We leveraged repeated measures over the course of an entire year to isolate the relative importance of environmental, host physiological, and substrate age-related factors on the structure of leaf-associated fungal communities. We applied both culturing and sequencing approaches to investigate these communities, focusing on a foundational, widely distributed plant of conservation concern: basin big sagebrush (Artemisia tridentata subsp. tridentata). We found that changes in alpha diversity were independently affected by the age of leaves and the air temperature. Total fungal abundance and species richness were not positively correlated and responded differently, sometimes oppositely, to weather. With regard to beta diversity, communities were more similar to each other across similar leaf ages, air temperatures, leaf types, and δ13C stable isotope ratios. Nine different genera were differentially abundant with air temperature, δ13C, leaf type, and leaf age, and a set of 20 genera were continuously present across the year. Our findings highlight the necessity for longer term, repeated sampling to parse drivers of temporal change in leaf microbial communities.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Ecosphere
Ecosphere ECOLOGY-
CiteScore
4.70
自引率
3.70%
发文量
378
审稿时长
15 weeks
期刊介绍: The scope of Ecosphere is as broad as the science of ecology itself. The journal welcomes submissions from all sub-disciplines of ecological science, as well as interdisciplinary studies relating to ecology. The journal''s goal is to provide a rapid-publication, online-only, open-access alternative to ESA''s other journals, while maintaining the rigorous standards of peer review for which ESA publications are renowned.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信